LOCUS       BBa_K1610302            1278 bp    DNA     linear       10-May-2024
DEFINITION  J23100 + RBS + yebF + GFP + Term
ACCESSION   BBa_K1610302
VERSION     BBa_K1610302.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..415
                     /label=BBa_K1610300
     CDS             422..1141
                     /label=GFP protein
     CDS             422..1141
                     /label=BBa_E0040
     stem_loop       1161..1204
                     /label=stem_loop
     misc_feature    1150..1229
                     /label=BBa_B0010
     terminator      1150..1229
                     /label=BBa_B0010
     stem_loop       1245..1264
                     /label=T7 TE
     misc_feature    1271..1271
                     /label=stop
     polyA_site      1265..1278
                     /label=polya
     misc_feature    1238..1278
                     /label=BBa_B0012
     terminator      1238..1278
                     /label=BBa_B0012
     terminator      1150..1278
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgaaaaaa agaggggcgt ttttagggct gttgttggtt tctgcctgcg catcagtttt
      121 cgctgccaat aatgaaacca gcaagtcggt cactttccca aagtgtgaag atctggatgc
      181 tgccggaatt gccgcgagcg taaaacgtga ttatcaacaa aatcgcgtgg cgcgttgggc
      241 agatgatcaa aaaattgtcg gtcaggccga tcccgtggct tgggtcagtt tgcaggacat
      301 tcagggtaaa gatgataaat ggtcagtacc gctaaccgtg cgtggtaaaa gtgccgatat
      361 tcattaccag gtcagcgtgg actgcaaagc gggaatggcg gaatatcagc ggcgttacta
      421 gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
      481 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      541 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      601 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca
      661 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
      721 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
      781 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
      841 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
      901 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
      961 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
     1021 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
     1081 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata
     1141 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     1201 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     1261 ggcctttctg cgtttata
//