LOCUS       BBa_K1613017            1569 bp    DNA     linear       26-Nov-2024
DEFINITION  K1613017 is a new part which can detect lead (Pb) ions in the 
            liquid.
ACCESSION   BBa_K1613017
VERSION     BBa_K1613017.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23102
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     CDS             63..461
                     /label=BBa_I721002
     stem_loop       481..524
                     /label=stem_loop
     misc_feature    470..549
                     /label=BBa_B0010
     terminator      470..549
                     /label=BBa_B0010
     stem_loop       565..584
                     /label=T7 TE
     misc_feature    591..591
                     /label=stop
     polyA_site      585..598
                     /label=polya
     misc_feature    558..598
                     /label=BBa_B0012
     terminator      558..598
                     /label=BBa_B0012
     terminator      470..598
                     /label=BBa_B0015
     promoter        607..700
                     /label=BBa_I721001
     misc_feature    713..716
                     /label=conserved
     RBS             709..720
                     /label=BBa_B0034
     CDS             727..1401
                     /label=mrfp1
     misc_feature    1408..1432
                     /label=Help:Barcodes
     CDS             727..1432
                     /label=BBa_E1010
     stem_loop       1452..1495
                     /label=stem_loop
     misc_feature    1441..1520
                     /label=BBa_B0010
     terminator      1441..1520
                     /label=BBa_B0010
     stem_loop       1536..1555
                     /label=T7 TE
     misc_feature    1562..1562
                     /label=stop
     polyA_site      1556..1569
                     /label=polya
     misc_feature    1529..1569
                     /label=BBa_B0012
     terminator      1529..1569
                     /label=BBa_B0012
     terminator      1441..1569
                     /label=BBa_B0015
ORIGIN
        1 ttgacagcta gctcagtcct aggtactgtg ctagctacta gagtcacaca ggaaagtact
       61 agatgatgcg gatcggtgaa ctgggcaaga aggcagattg cttggtgcag accgtgcgct
      121 tttacgagtc agaaggcttg ctgcccgagc ctgcacgtag cgagggcaac ttcaggctct
      181 atgacgaagt ccatttgcag cgcttgctgt tcatccgccg ctgccgggcg aaggacatga
      241 cgctggatga gatccgtcaa ctgctgaact tacgggatcg gccagagttg ggctgcggcg
      301 aggtgaacgc gctggtcgac gctcatatcg cgcaagtgcg gaccaagatg aaggaattgc
      361 gcgccttgga gcgcgagtta atggatctgc gacgctcctg cgatagcgcc cgaacctcgc
      421 gcgagtgcgg cattctcaac agcttggccg agcccgcctg atactagagc caggcatcaa
      481 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
      541 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata
      601 ctagagggtt gcttcctata aaaaacttga ctctatatct actagaggtt ttctaatgat
      661 ggcatccggg gaaaaccttg tcaatgaaga gcgatctatg tactagagaa agaggagaaa
      721 tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa agttcgtatg
      781 gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac
      841 gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg
      901 gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca cccggctgac
      961 atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg tgttatgaac
     1021 ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga cggtgagttc
     1081 atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt tatgcagaaa
     1141 aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa
     1201 ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc tgaagttaaa
     1261 accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac cgacatcaaa
     1321 ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga acgtgctgaa
     1381 ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc gctactagag
     1441 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1501 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1561 gcgtttata
//