BBa_K1613019
type: ComponentDefinition
persistent identity: BBa K1613019
display id: BBa_K1613019
version: 1
was generated by: iGEM to SBOL conversion
description: It can detect methyl mercury.
created: 2015-09-02T11:00:00Z
modified: 2015-11-12T09:16:56Z
mutable provenance: K1420002 k1420001
owned by: james
owned by: myers
mutable description: MerB can combine with methyl mercury and turns it into mercury ion. MerR can combine with mercury ion and further reaction can proceed successfully.
discontinued: true
dominant: false
experience: experience/None
group u list: _2030_
m user id: 26843
owner id: 26841
owning group id: 9
status: status/Deleted
bookmark: false
mutable notes: ttgacagctagctcagtcctaggtactgtgctagcTACTAGAGATGAAGCTCGCCCCATATATTTTAGAACGTCTCACTTCGGTCAATCGTACCAATGGTACTGCGGATCTCTTGGTCCCGCTACTGCGGGAACTCGCCAAGGGGCGTCCGGTTTCACGAACGACACTTGCCGGGATTCTCGACTGGCCCGCTGAGCGAGTGGCCGCCGTACTCGAACAGGCCACCAGTACCGAATATGACAAAGATGGGAACATCATCGGCTACGGCCTCACCTTGCGCGAGACTTCGTATGTCTTTGAAATTGACGACCGCCGTCTGTATGCCTGGTGCGCGCTGGACACCTTGATATTTCCGGCGCTGATCGGCCGTACAGCTCGCGTCTCATCGCATTGCGCTGCAACCGGAGCACCCGTTTCACTCACGGTTTCACCCAGCGAGATACAGGCTGTCGAACCTGCCGGCATGGCGGTGTCCTTGGTATTGCCGCAGGAAGCAGCCGACGTTCGTCAGTCCTTCTGTTGCCATGTACATTTCTTTGCATCTGTCCCGACGGCGGAAGACTGGGCCTCCAAGCATCAAGGATTGGAAGGATTGGCGATCGTCAGTGTCCACGAGGCTTTCGGCTTGGGCCAGGAGTTTAATCGACATCTGTTGCAGACCATGTCATCTAGGACACCGTGATACTAGAGAAAGAGGAGAAATACTAGAGTACTAGATGGAAAATAATTTGGAAAACCTGACCATTGGCGTTTTTGCCAAGGCGGCCGGGGTCAACGTGGAGACAATCCGCTTCTATCAGCGCAAGGGCCTGTTGCGGGAACCGGACAAGCCTTACGGCAGCATCCGCCGCTATGGGGAGGCGGACGTGGTTCGGGTGAAATTCGTGAAATCGGCACAGCGGCTGGGGTTCAGTCTGGACGAGATTGCCGAGCTGTTGCGGCTCGACGATGGCACCCACTGCGAGGAGGCCAGCAGCCTGGCCGAACACAAGCTCAAGGACGTGCGCGAGAAGATGGCCGACTTGGCGCGCATGGAAACCGTGCTGTCTGAACTCGTGTGCGCCTGCCATGCACGAAAGGGGAATGTTTCCTGCCCGTTGATCGCGTCACTACAGGGCGAAGCAGGCCTGGCAAGGTCAGCTATGCCTTAGTACTAGAGTTCCATATCGCTTGACTCCGTACATGAGTACGGAAGTAAGGTTACGCTATCCAATCCTACTAGAGTCACACAGGAAAGTACTAGAGATGGCTTCCTCCGAAGACGTTATCAAAGAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAAATCGAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTTACCAAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGTTCCAAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCGGAAGGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACCCAGGACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAACTTCCCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAACGTATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAAGACGGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAGCTGCCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTACACCATCGTTGAACAGTACGAACGTGCTGAAGGTCGTCACTCCACCGGTGCTTAATAACGCTGATAGTGCTAGTGTAGATCGCTACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGG…
star: false
creator: HSU, CHIA-YANG
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_J23102
component: BBa_K1420002
component: BBa_K346002
component: BBa_B0034
component: BBa_K1420004
component: BBa_E1010
component: BBa_B0032
component: BBa_B0015
sequence annotation: BBa_J23102
sequence annotation: BBa_B0034
sequence annotation: BBa_K1420002
sequence annotation: BBa_K1420004
sequence annotation: BBa_K346002
sequence annotation: BBa_B0032
sequence annotation: BBa_E1010
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1613019_sequence/1

BBa_J23102
type: Component
persistent identity: component2443021
display id: component2443021
version: 1
top level: BBa_K1613019
definition: BBa_J23102
access: public

BBa_K1420002
type: Component
persistent identity: component2443025
display id: component2443025
version: 1
top level: BBa_K1613019
definition: BBa_K1420002
access: public

BBa_K346002
type: Component
persistent identity: component2443029
display id: component2443029
version: 1
top level: BBa_K1613019
definition: BBa_K346002
access: public

BBa_B0034
type: Component
persistent identity: component2443023
display id: component2443023
version: 1
top level: BBa_K1613019
definition: BBa_B0034
access: public

BBa_K1420004
type: Component
persistent identity: component2443027
display id: component2443027
version: 1
top level: BBa_K1613019
definition: BBa_K1420004
access: public

BBa_E1010
type: Component
persistent identity: component2443035
display id: component2443035
version: 1
top level: BBa_K1613019
definition: mRFP1
access: public

BBa_B0032
type: Component
persistent identity: component2443031
display id: component2443031
version: 1
top level: BBa_K1613019
definition: BBa_B0032
access: public

BBa_B0015
type: Component
persistent identity: component2443042
display id: component2443042
version: 1
top level: BBa_K1613019
definition: BBa_B0015
access: public

BBa_J23102
type: SequenceAnnotation
persistent identity: annotation2443021
display id: annotation2443021
version: 1
top level: BBa_K1613019
location: https://synbiohub.org/public/igem/BBa_K1613019/annotation2443021/range2443021/1
component: BBa_J23102

https://synbiohub.org/public/igem/BBa_K1613019/annotation2443021/range2443021/1
type: Range
persistent identity: range2443021
display id: range2443021
version: 1
top level: BBa_K1613019
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2443023
display id: annotation2443023
version: 1
top level: BBa_K1613019
location: https://synbiohub.org/public/igem/BBa_K1613019/annotation2443023/range2443023/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1613019/annotation2443023/range2443023/1
type: Range
persistent identity: range2443023
display id: range2443023
version: 1
top level: BBa_K1613019
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_K1420002
type: SequenceAnnotation
persistent identity: annotation2443025
display id: annotation2443025
version: 1
top level: BBa_K1613019
location: https://synbiohub.org/public/igem/BBa_K1613019/annotation2443025/range2443025/1
component: BBa_K1420002

https://synbiohub.org/public/igem/BBa_K1613019/annotation2443025/range2443025/1
type: Range
persistent identity: range2443025
display id: range2443025
version: 1
top level: BBa_K1613019
direction: direction/reverse
start: 62
end: 700
orientation: inline

BBa_K1420004
type: SequenceAnnotation
persistent identity: annotation2443027
display id: annotation2443027
version: 1
top level: BBa_K1613019
location: https://synbiohub.org/public/igem/BBa_K1613019/annotation2443027/range2443027/1
component: BBa_K1420004

https://synbiohub.org/public/igem/BBa_K1613019/annotation2443027/range2443027/1
type: Range
persistent identity: range2443027
display id: range2443027
version: 1
top level: BBa_K1613019
direction: direction/reverse
start: 709
end: 1143
orientation: inline

BBa_K346002
type: SequenceAnnotation
persistent identity: annotation2443029
display id: annotation2443029
version: 1
top level: BBa_K1613019
location: https://synbiohub.org/public/igem/BBa_K1613019/annotation2443029/range2443029/1
component: BBa_K346002

https://synbiohub.org/public/igem/BBa_K1613019/annotation2443029/range2443029/1
type: Range
persistent identity: range2443029
display id: range2443029
version: 1
top level: BBa_K1613019
direction: direction/reverse
start: 1152
end: 1208
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2443031
display id: annotation2443031
version: 1
top level: BBa_K1613019
location: https://synbiohub.org/public/igem/BBa_K1613019/annotation2443031/range2443031/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1613019/annotation2443031/range2443031/1
type: Range
persistent identity: range2443031
display id: range2443031
version: 1
top level: BBa_K1613019
direction: direction/forward
start: 1217
end: 1229
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2443035
display id: annotation2443035
version: 1
top level: BBa_K1613019
location: https://synbiohub.org/public/igem/BBa_K1613019/annotation2443035/range2443035/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K1613019/annotation2443035/range2443035/1
type: Range
persistent identity: range2443035
display id: range2443035
version: 1
top level: BBa_K1613019
direction: direction/reverse
start: 1236
end: 1941
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2443042
display id: annotation2443042
version: 1
top level: BBa_K1613019
location: https://synbiohub.org/public/igem/BBa_K1613019/annotation2443042/range2443042/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1613019/annotation2443042/range2443042/1
type: Range
persistent identity: range2443042
display id: range2443042
version: 1
top level: BBa_K1613019
direction: direction/reverse
start: 1950
end: 2078
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_J23102
type: ComponentDefinition
persistent identity: BBa J23102
display id: BBa_J23102
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23102_sequence/1

BBa_K1420002
type: ComponentDefinition
persistent identity: BBa K1420002
display id: BBa_K1420002
version: 1
was generated by: iGEM to SBOL conversion
description: merB, Organomercurial Lyase from Serratia marcescens
created: 2014-10-08T11:00:00Z
modified: 2015-07-22T02:52:43Z
mutable provenance: Amplified from pDU1358, kindly donated by Anne O. Summers.
owned by: james
owned by: myers
mutable description: Demethylates organomerury.
discontinued: false
dominant: false
experience: experience/None
group u list: _1798_
m user id: 4206
owner id: 17872
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: false
creator: Stephen C. Heinsch
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: merB
sequence: https://synbiohub.org/public/igem/BBa_K1420002_sequence/1

merB
type: SequenceAnnotation
persistent identity: annotation2407616
display id: annotation2407616
version: 1
top level: BBa_K1420002
location: https://synbiohub.org/public/igem/BBa_K1420002/annotation2407616/range2407616/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1420002/annotation2407616/range2407616/1
type: Range
persistent identity: range2407616
display id: range2407616
version: 1
top level: BBa_K1420002
direction: direction/forward
start: 1
end: 639
orientation: inline

BBa_K1420004
type: ComponentDefinition
persistent identity: BBa K1420004
display id: BBa_K1420004
version: 1
was generated by: iGEM to SBOL conversion
description: merR family transcriptional regulator, regulatory protein for mer operon
created: 2014-10-08T11:00:00Z
modified: 2015-07-22T03:01:33Z
mutable provenance: Source organism: Serratia marcescens. Amplified from plasmid pDU1358, kindly donated by Anne O. Summers.
owned by: james
owned by: myers
mutable description: merR, located upstream of the rest of the mer genes, serves to regulate the operon. The MerR protein contains a C-terminal effector binding region that recognizes ionic mercury. Upon binding it, the protein???s N-terminal helix-turn-helix DNA binding domain can bind the merR promoter region to induce transcription of the operon. The effector binding region of the protein can vary allowing for great diversity in merR-like promoters that can respond to a variety of heavy metals as well as antibiotics and oxidative stress.
discontinued: false
dominant: false
experience: experience/None
group u list: _1798_
m user id: 4206
owner id: 17872
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Stephen C. Heinsch
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: merR
sequence: https://synbiohub.org/public/igem/BBa_K1420004_sequence/1

merR
type: SequenceAnnotation
persistent identity: annotation2422563
display id: annotation2422563
version: 1
top level: BBa_K1420004
location: https://synbiohub.org/public/igem/BBa_K1420004/annotation2422563/range2422563/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1420004/annotation2422563/range2422563/1
type: Range
persistent identity: range2422563
display id: range2422563
version: 1
top level: BBa_K1420004
direction: direction/forward
start: 1
end: 435
orientation: inline

BBa_K346002
type: ComponentDefinition
persistent identity: BBa K346002
display id: BBa_K346002
version: 1
was generated by: iGEM to SBOL conversion
description: PmerT promoter (mercury-responsive)
created: 2010-10-11T11:00:00Z
modified: 2015-07-14T12:33:51Z
mutable provenance: TTCCATATCGCTTGACTCCGTACATGAGTACGGAAGTAAGGTTACGCTATCCAATCC
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part was isolated from Tn21. When regulated by its cognate transcription factor MerR, it will represent a dose-response manner in response to the concentration of mercury.
discontinued: false
dominant: false
experience: experience/Works
group u list: _457_
m user id: 4206
owner id: 4411
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: It was divergently regulated by MerR
star: false
creator: Qianzhu Wu & Mei Chen
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_K346002_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2085551
display id: annotation2085551
version: 1
top level: BBa_K346002
location: https://synbiohub.org/public/igem/BBa_K346002/annotation2085551/range2085551/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K346002/annotation2085551/range2085551/1
type: Range
persistent identity: range2085551
display id: range2085551
version: 1
top level: BBa_K346002
direction: direction/forward
start: 1
end: 57
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1613019_sequence/1
type: Sequence
persistent identity: BBa K1613019 sequence
display id: BBa_K1613019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtactgtgctagctactagagaaagaggagaaatactagatgaagctcgccccatatattttagaacgtctcacttcggtcaatcgtaccaatggtactgcggatctcttggtcccgctactgcgggaactcgccaaggggcgtccggtttcacgaacgacacttgccgggattctcgactggcccgctgagcgagtggccgccgtactcgaacaggccaccagtaccgaatatgacaaagatgggaacatcatcggctacggcctcaccttgcgcgagacttcgtatgtctttgaaattgacgaccgccgtctgtatgcctggtgcgcgctggacaccttgatatttccggcgctgatcggccgtacagctcgcgtctcatcgcattgcgctgcaaccggagcacccgtttcactcacggtttcacccagcgagatacaggctgtcgaacctgccggcatggcggtgtccttggtattgccgcaggaagcagccgacgttcgtcagtccttctgttgccatgtacatttctttgcatctgtcccgacggcggaagactgggcctccaagcatcaaggattggaaggattggcgatcgtcagtgtccacgaggctttcggcttgggccaggagtttaatcgacatctgttgcagaccatgtcatctaggacaccgtgatactagagctacaccgcgtcggcaccacgcggctctttctccccttgcagcgaagcaatcagcgggcaagaaacgttcccttgccgcgcatggcaggcgaacacaagttcggatagcacggtttccatgcgcgccaggtcggtcattttttcgcgcacgtcctgaagcttgtgctcggccaggctgctggcttcctcgcagtgggtgccgtcatccagcctcagcagctctgcgatctcgtcgaggctgaatccgagccgctgggctgatttcacgaagcgcacccgcgtcacatccgcctcgccatagcggcggatgctgccatagggcttgtccggctccggcaacaagcccttgcgctgatagaaccggattgtttccacgttgaccccggccgccttggcgaaaacgccaatagtcagattctccaaatttttttccattactagagttccatatcgcttgactccgtacatgagtacggaagtaaggttacgctatccaatcctactagagtcacacaggaaagtactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23102_sequence/1
type: Sequence
persistent identity: BBa J23102 sequence
display id: BBa_J23102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtactgtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1420002_sequence/1
type: Sequence
persistent identity: BBa K1420002 sequence
display id: BBa_K1420002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaagctcgccccatatattttagaacgtctcacttcggtcaatcgtaccaatggtactgcggatctcttggtcccgctactgcgggaactcgccaaggggcgtccggtttcacgaacgacacttgccgggattctcgactggcccgctgagcgagtggccgccgtactcgaacaggccaccagtaccgaatatgacaaagatgggaacatcatcggctacggcctcaccttgcgcgagacttcgtatgtctttgaaattgacgaccgccgtctgtatgcctggtgcgcgctggacaccttgatatttccggcgctgatcggccgtacagctcgcgtctcatcgcattgcgctgcaaccggagcacccgtttcactcacggtttcacccagcgagatacaggctgtcgaacctgccggcatggcggtgtccttggtattgccgcaggaagcagccgacgttcgtcagtccttctgttgccatgtacatttctttgcatctgtcccgacggcggaagactgggcctccaagcatcaaggattggaaggattggcgatcgtcagtgtccacgaggctttcggcttgggccaggagtttaatcgacatctgttgcagaccatgtcatctaggacaccgtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1420004_sequence/1
type: Sequence
persistent identity: BBa K1420004 sequence
display id: BBa_K1420004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctacaccgcgtcggcaccacgcggctctttctccccttgcagcgaagcaatcagcgggcaagaaacgttcccttgccgcgcatggcaggcgaacacaagttcggatagcacggtttccatgcgcgccaggtcggtcattttttcgcgcacgtcctgaagcttgtgctcggccaggctgctggcttcctcgcagtgggtgccgtcatccagcctcagcagctctgcgatctcgtcgaggctgaatccgagccgctgggctgatttcacgaagcgcacccgcgtcacatccgcctcgccatagcggcggatgctgccatagggcttgtccggctccggcaacaagcccttgcgctgatagaaccggattgtttccacgttgaccccggccgccttggcgaaaacgccaatagtcagattctccaaatttttttccat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K346002_sequence/1
type: Sequence
persistent identity: BBa K346002 sequence
display id: BBa_K346002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttccatatcgcttgactccgtacatgagtacggaagtaaggttacgctatccaatcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z