BBa_K1614014 1 BBa_K1614014 ATP Aptamer JAWS1 Spinach2 2015-09-15T11:00:00Z 2015-09-18T09:22:07Z Synthentic created with JAWS with Spinach II aptamer (Babendure, J.R., Adams, S.R., and Tsien, R.Y. (2003). Aptamers switch on fluorescence of triphenylmethane dyes. Journal of the American Chemical Society 125, 14716-14717.) and ATP aptamer Created from Part:BBa_K1614011 to sense ATP with a fluorescent read out. false false _2031_ 16422 24664 9 false Cloned without restriction enzymes to avoid issues occurring from conserved cut sites. For more details see RFC 110. false Frieda Anna Sorgenfrei annotation2459085 1 optimized stem part2 range2459085 1 131 138 annotation2459068 1 Spinach II part1 range2459068 1 31 65 annotation2459060 1 G required for transcription range2459060 1 30 30 annotation2459086 1 Spinach II part2 range2459086 1 139 176 annotation2459087 1 HDV range2459087 1 177 261 annotation2459069 1 optimized stem part1 range2459069 1 66 73 annotation2459067 1 T7 promotor range2459067 1 13 29 annotation2459070 1 ATP aptamer range2459070 1 74 130 BBa_K1614014_sequence 1 gatatcgcgcgctaatacgactcactataggatgtaactgaatgaaatggtgaaggacgggtccagggccagggggagatctacggatctcagggctcttacgggagctacatggaaggagtccatgtgtctttgcccttgttgagtagagtgtgagctccgtaactagttacatcggccggcatggtcccagcctcctcgctggcgccggctgggcaacattccgaggggaccgtcccctcggtaatggcgaatgggacgatatc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z