BBa_K1614016
1
BBa_K1614016
Hammerhead Ribozyme and Malchite Green Aptamer in BBF RFC 110 transcription cassette
2015-09-15T11:00:00Z
2015-09-16T05:09:22Z
Synthetic, Malchit Green aptamer (Grate, D., and Wilson, C. (1999). Laser-mediated, site-specific inactivation of RNA transcripts. Proceedings of the National Academy of Sciences of the United States of America 96, 6131-6136.) HHR (Prody, G.A., Bakos, J.T., Buzayan, J.M., Schneider, I.R., and Bruening, G. (1986). Autolytic processing of dimeric plant virus satellite RNA. Science 231, 1577-1580)
Hammerhead Ribozyme (HHR) and Malchite Green (MG) Aptamer in BBF RFC 110 transcription cassette for live tracking of <i>in vitro</i> transcription.
false
false
_2031_
24664
24664
9
false
Cloned without restriction enzymes to avoid issues occurring from conserved cut sites. For more details see BBF RFC 110.
false
Frieda Anna Sorgenfrei
annotation2459467
1
G required for transcription
range2459467
1
30
30
annotation2459506
1
HDV
range2459506
1
123
206
annotation2459490
1
Malachite Green Aptamer
range2459490
1
85
122
annotation2459466
1
T7 promotor
range2459466
1
13
29
annotation2459489
1
HHR
range2459489
1
31
84
BBa_K1614016_sequence
1
gatatcgcgcgctaatacgactcactatagtccagtcaccggatgtgctttccggtctgatgagtccgtgaggacgaaactggtggatcccgactggcgagagccaggtaacgaatggatccggccggcatggtcccagcctcctcgctggcgccggctgggcaacattccgaggggaccgtcccctcggtaatggcgaatgggacgatatc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z