BBa_K1614025 1 BBa_K1614025 Ketamin-dependent self cleaving F8 - readout DNAzyme 2015-09-17T11:00:00Z 2015-09-18T07:05:08Z Synthetic. Construct consisting of a ketamin-switched F8 DNAzyme linked to a stemmed HRP mimicking DNAzyme. If there no ketamine binds to aptamer the F8 is inactive and HRP mimicking DNAzyme is stemmed, so that no hemin could bind and there is no peroxidase activity. By binding of ketamin to the aptamer, F8 is activated and cleaves the stemming part, so the HRP mimicking DNAzyme is activated, peroxidase activity could be used for different readout techniques. Stems and Aptamer generated by MAWS and JAWS software. false false _2031_ 24635 24635 9 false Aptamer generated by MAWS software. Stems optimized by JAWS software. false Daniel Heid BBa_K1614025_sequence 1 ggttggtgtggttggaaaaatgccgatccatactgcggaacccgggtagggcgggttgggttccgtggatgcctattccgatgggtagaccgggtccgtatggatcggca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z