BBa_B0105 1 Scar 25 RFC 25 Scar Sequence 2009-10-14T11:00:00Z 2015-08-31T04:07:21Z BBF RFC 25 This is the scar produced by assembly using RFC 25. If you are assembling a composite part using RFC 25, you can insert this part and specify blunt assembly to get the desired sequence. false false _1_ 0 25 397 Not in stock false Simple DNA sequence false Randy Rettberg annotation2041621 1 Scar 25 range2041621 1 1 6 BBa_K1615000 1 MorA Morphine-6-dehydrogenase RFC25 2015-08-26T11:00:00Z 2015-08-28T03:12:05Z Genomic sequence from Pseudomonas putida. Morphine-6-dehydrogenase. NADP dependent oxidoreductase. Oxidises morphine to morphinone. false false _2032_ 25547 25547 9 false Codon optimised for E. coli. RFC 25 compatible. false Brooke Rothschild-Mancinelli annotation2438528 1 Morphine-6-dehydrogenase range2438528 1 1 882 BBa_K1321002 1 BBa_K1321002 Re-entry of BBa_K863111, Cellulose Binding Domain CBDclos in rfc25 2014-10-09T11:00:00Z 2015-06-17T12:35:41Z This is a direct copy of BBa_K863111 with slight modifications to the beginning and end of the sequence for the purposes of allowing composite parts to be added correctly using this part. This part is a copy of BBa_K863111 (Cellulose binding domain of C. cellulovorans cellulose binding protein gene (Freiburg-Standard)) and was created only as a registry entry, removing the atg-NgoMIV parts at the front and the AgeI-taa basepairs from the sequence so that the registry does not flag it as rfc25 incompatible, and it enables us to enter fusion proteins with this part as automatic composite parts in the method as described by: http://2009.igem.org/Team:Freiburg_bioware/general. false false _1696_ 4206 20780 9 Not in stock false This is a direct copy of BBa_K863111 with slight modifications to the beginning and end of the sequence for the purposes of allowing composite parts to be added correctly using this part. false Xenia Spencer-Milnes BBa_K1615008 1 BBa_K1615008 CBDclos fused to MorA in RFC25 2015-08-26T11:00:00Z 2015-08-27T04:01:57Z Morphine-6-dehydrogenase from Pseudomonas putida. CBDclos from Clostridium cellulovorans. Morphine-6-dehydrogenase fused C terminally to CBDclos. false false _2032_ 25547 25547 9 false Fusion done using RFC25 false Brooke Rothschild-Mancinelli component2438787 1 BBa_K1615000 component2438785 1 BBa_B0105 component2438783 1 BBa_K1321002 annotation2438783 1 BBa_K1321002 range2438783 1 1 300 annotation2438785 1 BBa_B0105 range2438785 1 301 306 annotation2438787 1 BBa_K1615000 range2438787 1 307 1188 BBa_B0105_sequence 1 accggc BBa_K1615008_sequence 1 tcatcaatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacgtctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagcaccggcgcaggtaaaagtccgctgattaatctgaataatggcgttaaaatgcctgcactgggtctgggtgtttttgcagcaagcgcagaagaaaccgcaagcgcaattgcaagcgccattagcagcggttatcgtctgattgataccgcacgtagctataacaatgaagcacaggttggtgaaggtattcgtaatagcgttgatcgtgcagaaatgtttgttaccaccaaactgttcaattgcgattatggttatgaacgtgcactgcgtgcatttgatgaaagcctgggtcgtctgggcctggattatgttgatctgtatctgctgcattggccgaccaaagattggaatgcaaccattcagagctggaaagcagcagaaaaaattctgggtgatggtcgtgcacgtgccattggtgtttgtaattttctggaagatcagctggatgaactgattgcagcatcagatgttgttccggcagttaatcagatcgaactgcatccgtattttgcacagaaacctctgctggcaaaaaatcgtgccctgggtattgttaccgaagcatggtcaccgattggttgtcatcagcgttggcaggatggtgataatcatggtggtcgtaaacatccgctgaccgatccggttattaccaccattgccgaagcacatggtcgtagcgcagcccaggttattctgcgttggcattttcagaatgatgttgttgcaattccgaaaagcgttaatccggaacgtattgccaaaaacattgatgtgtttgattttgccctgtccgatgccgaaatggcacagctggacgagctggatacaggtgttcgtattggtccggatccgcgtgatgttgataccagcagctttgcagaatttgtg BBa_K1321002_sequence 1 tcatcaatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacgtctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagc BBa_K1615000_sequence 1 gcaggtaaaagtccgctgattaatctgaataatggcgttaaaatgcctgcactgggtctgggtgtttttgcagcaagcgcagaagaaaccgcaagcgcaattgcaagcgccattagcagcggttatcgtctgattgataccgcacgtagctataacaatgaagcacaggttggtgaaggtattcgtaatagcgttgatcgtgcagaaatgtttgttaccaccaaactgttcaattgcgattatggttatgaacgtgcactgcgtgcatttgatgaaagcctgggtcgtctgggcctggattatgttgatctgtatctgctgcattggccgaccaaagattggaatgcaaccattcagagctggaaagcagcagaaaaaattctgggtgatggtcgtgcacgtgccattggtgtttgtaattttctggaagatcagctggatgaactgattgcagcatcagatgttgttccggcagttaatcagatcgaactgcatccgtattttgcacagaaacctctgctggcaaaaaatcgtgccctgggtattgttaccgaagcatggtcaccgattggttgtcatcagcgttggcaggatggtgataatcatggtggtcgtaaacatccgctgaccgatccggttattaccaccattgccgaagcacatggtcgtagcgcagcccaggttattctgcgttggcattttcagaatgatgttgttgcaattccgaaaagcgttaatccggaacgtattgccaaaaacattgatgtgtttgattttgccctgtccgatgccgaaatggcacagctggacgagctggatacaggtgttcgtattggtccggatccgcgtgatgttgataccagcagctttgcagaatttgtg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z