LOCUS BBa_K1615018 2841 bp DNA linear 22-Nov-2024 DEFINITION CBDclos fused to MorA driven by LacI promoter ACCESSION BBa_K1615018 VERSION BBa_K1615018.1 FEATURES Location/Qualifiers misc_feature 18..324 /label=f1 origin misc_feature 1..455 /label=BBa_K314110 misc_feature 464..1555 /label=BBa_K314111 misc_feature 1566..1582 /label=lac O1 promoter 1583..1588 /label=-35 misc_feature 1589..1605 /label=lac O1 promoter 1606..1611 /label=-10 misc_feature 1564..1617 /label=BBa_R0011 promoter 1564..1617 /label=BBa_R0011 misc_feature 1631..1634 /label=conserved RBS 1627..1638 /label=BBa_B0034 misc_feature 1..1638 /label=BBa_K314103 misc_feature 1645..1647 /label=start misc_feature 1639..1653 /label=BBa_K1319106 CDS 1654..1953 /label=BBa_K1321002 misc_feature 1954..1959 /label=Scar 25 misc_feature 1954..1959 /label=BBa_B0105 CDS 1960..2841 /label=Morphine-6-dehydrogenase CDS 1960..2841 /label=BBa_K1615000 misc_feature 1654..2841 /label=BBa_K1615008 ORIGIN 1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 421 acgcgaattt taacaaaata ttaacgttta caatttacta gaggtggtga atgtgaaacc 481 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt 541 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc 601 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct 661 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat 721 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg 781 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat 841 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt 901 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca 961 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc 1021 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa 1081 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat 1141 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct 1201 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg 1261 cgttggtgcg gacatctcgg tagtgggata cgacgatacc gaagacagct catgttatat 1321 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg 1381 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact 1441 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 1501 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgatacta 1561 gagaattgtg agcggataac aattgacatt gtgagcggat aacaagatac tgagcacata 1621 ctagagaaag aggagaaata ctagatggcc ggctcatcaa tgtcagttga attttacaac 1681 tctaacaaat cagcacaaac aaactcaatt acaccaataa tcaaaattac taacacgtct 1741 gacagtgatt taaatttaaa tgacgtaaaa gttagatatt attacacaag tgatggtaca 1801 caaggacaaa ctttctggtg tgaccatgct ggtgcattat taggaaatag ctatgttgat 1861 aacactagca aagtgacagc aaacttcgtt aaagaaacag caagcccaac atcaacctat 1921 gatacatatg ttgaatttgg atttgcaggc agcaccggcg caggtaaaag tccgctgatt 1981 aatctgaata atggcgttaa aatgcctgca ctgggtctgg gtgtttttgc agcaagcgca 2041 gaagaaaccg caagcgcaat tgcaagcgcc attagcagcg gttatcgtct gattgatacc 2101 gcacgtagct ataacaatga agcacaggtt ggtgaaggta ttcgtaatag cgttgatcgt 2161 gcagaaatgt ttgttaccac caaactgttc aattgcgatt atggttatga acgtgcactg 2221 cgtgcatttg atgaaagcct gggtcgtctg ggcctggatt atgttgatct gtatctgctg 2281 cattggccga ccaaagattg gaatgcaacc attcagagct ggaaagcagc agaaaaaatt 2341 ctgggtgatg gtcgtgcacg tgccattggt gtttgtaatt ttctggaaga tcagctggat 2401 gaactgattg cagcatcaga tgttgttccg gcagttaatc agatcgaact gcatccgtat 2461 tttgcacaga aacctctgct ggcaaaaaat cgtgccctgg gtattgttac cgaagcatgg 2521 tcaccgattg gttgtcatca gcgttggcag gatggtgata atcatggtgg tcgtaaacat 2581 ccgctgaccg atccggttat taccaccatt gccgaagcac atggtcgtag cgcagcccag 2641 gttattctgc gttggcattt tcagaatgat gttgttgcaa ttccgaaaag cgttaatccg 2701 gaacgtattg ccaaaaacat tgatgtgttt gattttgccc tgtccgatgc cgaaatggca 2761 cagctggacg agctggatac aggtgttcgt attggtccgg atccgcgtga tgttgatacc 2821 agcagctttg cagaatttgt g //