BBa_R0011 1 lacI+pL Promoter (lacI regulated, lambda pL hybrid) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z represillator of Elowitz and Leibler (2000) Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference). false true _1_ 0 24 7 In stock false <P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs. true Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton annotation2001 1 lac O1 range2001 1 26 42 annotation1999 1 lac O1 range1999 1 3 19 annotation2002 1 -10 range2002 1 43 48 annotation7064 1 BBa_R0011 range7064 1 1 54 annotation2000 1 -35 range2000 1 20 25 BBa_K1319106 1 Scar25 ATG RFC 25 Translation Start Scar Sequence 2014-10-02T11:00:00Z 2015-05-08T01:09:51Z This sequence is produced by an assembly method. This sequence is produced by RFC10-assembly of a RFC10-RBS with a RFC25-prefixed part. false false _1694_ 0 19522 9 Not in stock false The resulting sequence is a combination of the RFC10 RBS-CDS scar (tactag) and 9 nucleotides from the RFC25 prefix (atg gcc ggc). false Michael Osthege annotation2393317 1 start range2393317 1 7 9 BBa_K314111 1 BBa_K314111 Lac I 2010-10-07T11:00:00Z 2015-05-08T01:11:55Z Pet 29 vector Lac I gene with promoter and RBS false false _496_ 0 6395 9 It's complicated false false IGEM 2010 Team Washington BBa_K1615022 1 MaoA Monoamine Oxidase A RFC25 2015-08-27T11:00:00Z 2015-08-28T03:08:31Z Codon optimised for E. coli. RFC 25 compatible Monoamine Oxidase A from Klebsiella pneumoniae. Copper dependent oxidase. Oxidises primary monoamines. false false _2032_ 25547 25547 9 false Codon optimised for E. coli. RFC 25 compatible. false Brooke Rothschild-Mancinelli annotation2439170 1 MaoA range2439170 1 1 2256 BBa_K314110 1 f1 ori f1 origin 2010-10-07T11:00:00Z 2015-05-08T01:11:55Z Pet 29 vector Released HQ 2013 Phage origin recognized by M13. Used to make SS DNA when infected with M13k07 helper phage false false _496_ 0 6395 9 In stock true false IGEM 2010 Team Washington annotation2115768 1 f1 origin range2115768 1 18 324 BBa_K314103 1 BBa_K314103 Lac induced expression cassette 2010-10-07T11:00:00Z 2015-05-08T01:11:55Z construct Released HQ 2013 Lac induced protein expression insert includes f1 origin, Lac I gene and RBS false false _496_ 0 6395 9 In stock true false Tomas Huber, Justin Siegel component2091081 1 BBa_K314110 component2091082 1 BBa_K314111 component2091089 1 BBa_B0034 component2091083 1 BBa_R0011 annotation2091081 1 BBa_K314110 range2091081 1 1 455 annotation2091083 1 BBa_R0011 range2091083 1 1564 1617 annotation2091082 1 BBa_K314111 range2091082 1 464 1555 annotation2091089 1 BBa_B0034 range2091089 1 1627 1638 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1615023 1 BBa_K1615023 Monoamine Oxidase A driven by LacI promoter 2015-08-27T11:00:00Z 2015-08-28T08:04:57Z Monoamine Oxidase A from Klebsiella pneumoniae. Lac expression cassette from iGEM10_Washington. Monoamine Oxidase A driven by the IPTG inducible PLlac 0-1 promoter. false false _2032_ 25547 25547 9 false Fusion was performed using RFC10 standard due to illegal NgoMIV site in f1 origin. false Brooke Rothschild-Mancinelli component2439381 1 BBa_K1615022 component2439377 1 BBa_K314103 component2439379 1 BBa_K1319106 annotation2439381 1 BBa_K1615022 range2439381 1 1654 3909 annotation2439377 1 BBa_K314103 range2439377 1 1 1638 annotation2439379 1 BBa_K1319106 range2439379 1 1639 1653 BBa_K314110_sequence 1 acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatt BBa_K1615023_sequence 1 acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagaggtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggacatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatggccggcgcaaatggtctgaaatttagtccgcgtaaagatgcactggcagttgcagttgtttgtgcatggcagagtccggtttttgcacatggtagcgaagcacatatggttccgctggataaaaccctgcaagaatttggtgcagatgttcagtgggatgattatgcacagatgtttaccctgattaaagatggtgcctacgttaaagttaaaccgggtgcaaaagatgccattgtgaatggtaaaagcctggatctgccggttccggttgttatgaaagaaggtaaagcctgggttagcgatacctttattaacgatgtttttcagagcggtctggatcagacctttcaggttgaaaaacgtccgcatccgctgaatagcctgagcgcagcagaaatttcaaaagcagttaccattgttaaagcagcccctgaatttcagccgaatacccgttttaccgaaattagcctgcatgaaccggataaagcagcagtttgggcatttgcactgcaaggcactccggttgatgcaccgcgtaccgcagatgttgttatgctggatggtaaacatgttattgaagccgttgtggacctgcaaaacaaaaaaatcctgagctggaccccgattaaaggtgcacataaaatggtgctgctggatgattttgttagcgtgcagaatattatcaacaccagcagcgaatttgccgaggttctgaaaaaacatggtattaccgatccgggtctggttgttaccacaccgctgaccgttggtttttttgatggtaaagatggcctgcaacaggatgcacgtctgctgaaagttgttagctatctggatacaggtgatggcaattattgggcacatccgattgaaaatctggttgccgttgttgatctggaagcgaaaaaaatcatcaaaatcgaagaaggtccggttattccggtgccgatggaaccgcgtccgtatgatggtcgtgatcgtaatgcaccggcagtgaaaccgctggaaattaccgaaccgaccggcaaaaactataccattacaggtgataccattcactggcagaattgggattttcatctgcgtctgaatagccgtgttggtccgattctgagcaccgttacctataatgataatggcaccaaacgtcaggtgatgtatgaaggtagcctgggtggtatgattgttccgtatggtgatccggatgttggttggtatttcaaagcatatctggatagcggtgattatggtatgggcaccctgaccagcccgattgttcgtggtaaagatgcaccgagcaatgcagttctgctggatgaaaccattgcagattataccggcaaaccgaccaccattccgggtgcagttgcaatttttgaacgttatgcaggtccggaatacaaacatctggaaatgggtaaaccgaatgttagcaccgaacgtcgtgaactggttgttcgttggattagcaccgttggtaactatgattatatcttcgattgggtgtttcacgataatgggaccattggtattgatgccggtgcaacaggtattgaagcagttaaaggtgttctggcaaaaaccatgcatgatccgagcgcaaaagaagatacccgttatggtacactgattgatcataatattgttggcaccacccatcagcacatttataactttcgtctggatctggatgttgatggcgaaaataacaccctggttgcaatggatgtggaagtgaaaccgaataccgcaggcggtccgcgtaccagcaccatgcaggttaatcagtatacaattgatagcgaacagaaagcagcccagaaatttgatccgggtacaattcgtctgctgagcaataccagcaaagaaaatcgtatgggtaatccggttagctatcagattatcccgtatgccggtggcacccatccggcagcaacaggtgcaaaatttgctccggatgaatggatttatcatcgtctgagctttatggataaacagctgtgggtgacccgttatcatccgaccgaacgttatccggaaggtaaatatccgaatcgtagcgcacatgatacaggtctgggtcagtatgcaaaagatgatgaaagcctgaccaatcatgatgatgttgtttggattaccacaggtacaacccatgttgcacgtgccgaagaatggccgattatgccgaccgaatgggcactggcactgctgaaaccgtggaacttttttgatgaaaccccgaccctgggtgagaaaaaaaaa BBa_B0034_sequence 1 aaagaggagaaa BBa_K1615022_sequence 1 gcaaatggtctgaaatttagtccgcgtaaagatgcactggcagttgcagttgtttgtgcatggcagagtccggtttttgcacatggtagcgaagcacatatggttccgctggataaaaccctgcaagaatttggtgcagatgttcagtgggatgattatgcacagatgtttaccctgattaaagatggtgcctacgttaaagttaaaccgggtgcaaaagatgccattgtgaatggtaaaagcctggatctgccggttccggttgttatgaaagaaggtaaagcctgggttagcgatacctttattaacgatgtttttcagagcggtctggatcagacctttcaggttgaaaaacgtccgcatccgctgaatagcctgagcgcagcagaaatttcaaaagcagttaccattgttaaagcagcccctgaatttcagccgaatacccgttttaccgaaattagcctgcatgaaccggataaagcagcagtttgggcatttgcactgcaaggcactccggttgatgcaccgcgtaccgcagatgttgttatgctggatggtaaacatgttattgaagccgttgtggacctgcaaaacaaaaaaatcctgagctggaccccgattaaaggtgcacataaaatggtgctgctggatgattttgttagcgtgcagaatattatcaacaccagcagcgaatttgccgaggttctgaaaaaacatggtattaccgatccgggtctggttgttaccacaccgctgaccgttggtttttttgatggtaaagatggcctgcaacaggatgcacgtctgctgaaagttgttagctatctggatacaggtgatggcaattattgggcacatccgattgaaaatctggttgccgttgttgatctggaagcgaaaaaaatcatcaaaatcgaagaaggtccggttattccggtgccgatggaaccgcgtccgtatgatggtcgtgatcgtaatgcaccggcagtgaaaccgctggaaattaccgaaccgaccggcaaaaactataccattacaggtgataccattcactggcagaattgggattttcatctgcgtctgaatagccgtgttggtccgattctgagcaccgttacctataatgataatggcaccaaacgtcaggtgatgtatgaaggtagcctgggtggtatgattgttccgtatggtgatccggatgttggttggtatttcaaagcatatctggatagcggtgattatggtatgggcaccctgaccagcccgattgttcgtggtaaagatgcaccgagcaatgcagttctgctggatgaaaccattgcagattataccggcaaaccgaccaccattccgggtgcagttgcaatttttgaacgttatgcaggtccggaatacaaacatctggaaatgggtaaaccgaatgttagcaccgaacgtcgtgaactggttgttcgttggattagcaccgttggtaactatgattatatcttcgattgggtgtttcacgataatgggaccattggtattgatgccggtgcaacaggtattgaagcagttaaaggtgttctggcaaaaaccatgcatgatccgagcgcaaaagaagatacccgttatggtacactgattgatcataatattgttggcaccacccatcagcacatttataactttcgtctggatctggatgttgatggcgaaaataacaccctggttgcaatggatgtggaagtgaaaccgaataccgcaggcggtccgcgtaccagcaccatgcaggttaatcagtatacaattgatagcgaacagaaagcagcccagaaatttgatccgggtacaattcgtctgctgagcaataccagcaaagaaaatcgtatgggtaatccggttagctatcagattatcccgtatgccggtggcacccatccggcagcaacaggtgcaaaatttgctccggatgaatggatttatcatcgtctgagctttatggataaacagctgtgggtgacccgttatcatccgaccgaacgttatccggaaggtaaatatccgaatcgtagcgcacatgatacaggtctgggtcagtatgcaaaagatgatgaaagcctgaccaatcatgatgatgttgtttggattaccacaggtacaacccatgttgcacgtgccgaagaatggccgattatgccgaccgaatgggcactggcactgctgaaaccgtggaacttttttgatgaaaccccgaccctgggtgagaaaaaaaaa BBa_K1319106_sequence 1 tactagatggccggc BBa_K314111_sequence 1 gtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggacatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtga BBa_R0011_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca BBa_K314103_sequence 1 acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagaggtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggacatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z