LOCUS BBa_K1615035 3777 bp DNA linear 29-Nov-2024 DEFINITION MaoA fused to CBDcenA+Linker in RFC25 driven by LacI promoter ACCESSION BBa_K1615035 VERSION BBa_K1615035.1 FEATURES Location/Qualifiers misc_feature 1..1092 /label=BBa_K314111 misc_feature 1099..1101 /label=start misc_feature 1093..1107 /label=BBa_K1319106 CDS 1108..3363 /label=MaoA CDS 1108..3363 /label=BBa_K1615022 misc_feature 3364..3369 /label=Scar 25 misc_feature 3364..3369 /label=BBa_B0105 CDS 3688..3777 /label=Linker: Pro-Thr box CDS 3370..3687 /label=CBDCenA CDS 3370..3777 /label=BBa_K1321339 misc_feature 1108..3777 /label=BBa_K1615025 ORIGIN 1 gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat 61 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa 121 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg 181 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg 241 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg 301 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa 361 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa 421 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac 481 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg 541 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt 601 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa 661 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc 721 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt 781 accgagtccg ggctgcgcgt tggtgcggac atctcggtag tgggatacga cgataccgaa 841 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg 901 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag 961 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc 1021 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 1081 agcgggcagt gatactagat ggccggcgca aatggtctga aatttagtcc gcgtaaagat 1141 gcactggcag ttgcagttgt ttgtgcatgg cagagtccgg tttttgcaca tggtagcgaa 1201 gcacatatgg ttccgctgga taaaaccctg caagaatttg gtgcagatgt tcagtgggat 1261 gattatgcac agatgtttac cctgattaaa gatggtgcct acgttaaagt taaaccgggt 1321 gcaaaagatg ccattgtgaa tggtaaaagc ctggatctgc cggttccggt tgttatgaaa 1381 gaaggtaaag cctgggttag cgataccttt attaacgatg tttttcagag cggtctggat 1441 cagacctttc aggttgaaaa acgtccgcat ccgctgaata gcctgagcgc agcagaaatt 1501 tcaaaagcag ttaccattgt taaagcagcc cctgaatttc agccgaatac ccgttttacc 1561 gaaattagcc tgcatgaacc ggataaagca gcagtttggg catttgcact gcaaggcact 1621 ccggttgatg caccgcgtac cgcagatgtt gttatgctgg atggtaaaca tgttattgaa 1681 gccgttgtgg acctgcaaaa caaaaaaatc ctgagctgga ccccgattaa aggtgcacat 1741 aaaatggtgc tgctggatga ttttgttagc gtgcagaata ttatcaacac cagcagcgaa 1801 tttgccgagg ttctgaaaaa acatggtatt accgatccgg gtctggttgt taccacaccg 1861 ctgaccgttg gtttttttga tggtaaagat ggcctgcaac aggatgcacg tctgctgaaa 1921 gttgttagct atctggatac aggtgatggc aattattggg cacatccgat tgaaaatctg 1981 gttgccgttg ttgatctgga agcgaaaaaa atcatcaaaa tcgaagaagg tccggttatt 2041 ccggtgccga tggaaccgcg tccgtatgat ggtcgtgatc gtaatgcacc ggcagtgaaa 2101 ccgctggaaa ttaccgaacc gaccggcaaa aactatacca ttacaggtga taccattcac 2161 tggcagaatt gggattttca tctgcgtctg aatagccgtg ttggtccgat tctgagcacc 2221 gttacctata atgataatgg caccaaacgt caggtgatgt atgaaggtag cctgggtggt 2281 atgattgttc cgtatggtga tccggatgtt ggttggtatt tcaaagcata tctggatagc 2341 ggtgattatg gtatgggcac cctgaccagc ccgattgttc gtggtaaaga tgcaccgagc 2401 aatgcagttc tgctggatga aaccattgca gattataccg gcaaaccgac caccattccg 2461 ggtgcagttg caatttttga acgttatgca ggtccggaat acaaacatct ggaaatgggt 2521 aaaccgaatg ttagcaccga acgtcgtgaa ctggttgttc gttggattag caccgttggt 2581 aactatgatt atatcttcga ttgggtgttt cacgataatg ggaccattgg tattgatgcc 2641 ggtgcaacag gtattgaagc agttaaaggt gttctggcaa aaaccatgca tgatccgagc 2701 gcaaaagaag atacccgtta tggtacactg attgatcata atattgttgg caccacccat 2761 cagcacattt ataactttcg tctggatctg gatgttgatg gcgaaaataa caccctggtt 2821 gcaatggatg tggaagtgaa accgaatacc gcaggcggtc cgcgtaccag caccatgcag 2881 gttaatcagt atacaattga tagcgaacag aaagcagccc agaaatttga tccgggtaca 2941 attcgtctgc tgagcaatac cagcaaagaa aatcgtatgg gtaatccggt tagctatcag 3001 attatcccgt atgccggtgg cacccatccg gcagcaacag gtgcaaaatt tgctccggat 3061 gaatggattt atcatcgtct gagctttatg gataaacagc tgtgggtgac ccgttatcat 3121 ccgaccgaac gttatccgga aggtaaatat ccgaatcgta gcgcacatga tacaggtctg 3181 ggtcagtatg caaaagatga tgaaagcctg accaatcatg atgatgttgt ttggattacc 3241 acaggtacaa cccatgttgc acgtgccgaa gaatggccga ttatgccgac cgaatgggca 3301 ctggcactgc tgaaaccgtg gaactttttt gatgaaaccc cgaccctggg tgagaaaaaa 3361 aaaaccggcg cgccgggttg ccgtgttgac tacgcggtta ccaaccagtg gccgggtggt 3421 ttcggtgcga acgttaccat caccaacctg ggtgacccgg tttcttcttg gaaactggac 3481 tggacctaca ccgcgggtca gcgtatccag cagctgtgga acggtaccgc gtctaccaac 3541 ggtggtcagg tttctgttac ctctctgccg tggaacggtt ctatcccgac tggtggtacc 3601 gcgtctttcg gtttcaacgg ttcttgggcg ggttctaacc cgaccccggc gtctttctct 3661 ctgaacggta ccacctgcac gggtaccccg acgacctcgc caactcctac gccgacccca 3721 accacgccga ctcctacacc gacgcccacc ccaacgccta ctccgacggt caccccc //