BBa_K1615025 1 BBa_K1615025 MaoA fused to CBDcenA+Linker in RFC25 2015-08-27T11:00:00Z 2015-08-28T08:13:04Z Monoamine Oxidase A from Klebsiella pneumoniae. CBDcenA from Cellulomonas fimi. Monoamine Oxidase A fused N terminally to CBDcenA + C terminal linker false false _2032_ 25547 25547 9 false Fusion done using RFC25 false Brooke Rothschild-Mancinelli component2439410 1 BBa_B0105 component2439413 1 BBa_K1321339 component2439408 1 BBa_K1615022 annotation2439408 1 BBa_K1615022 range2439408 1 1 2256 annotation2439413 1 BBa_K1321339 range2439413 1 2263 2670 annotation2439410 1 BBa_B0105 range2439410 1 2257 2262 BBa_K314111 1 BBa_K314111 Lac I 2010-10-07T11:00:00Z 2015-05-08T01:11:55Z Pet 29 vector Lac I gene with promoter and RBS false false _496_ 0 6395 9 It's complicated false false IGEM 2010 Team Washington BBa_K1615022 1 MaoA Monoamine Oxidase A RFC25 2015-08-27T11:00:00Z 2015-08-28T03:08:31Z Codon optimised for E. coli. RFC 25 compatible Monoamine Oxidase A from Klebsiella pneumoniae. Copper dependent oxidase. Oxidises primary monoamines. false false _2032_ 25547 25547 9 false Codon optimised for E. coli. RFC 25 compatible. false Brooke Rothschild-Mancinelli annotation2439170 1 MaoA range2439170 1 1 2256 BBa_K1615035 1 BBa_K1615035 MaoA fused to CBDcenA+Linker in RFC25 driven by LacI promoter 2015-08-31T11:00:00Z 2015-09-01T04:40:01Z Monoamine Oxidase A from Klebsiella pneumoniae. CBDcenA from Cellulomonas fimi. Lac expression cassette from iGEM10_Washington. Monoamine oxidase A fused N terminally to CBDcenA + C terminal linker driven by the IPTG inducible PLlac 0-1 promoter. false false _2032_ 25547 25547 9 false Fusion was performed using RFC10 standard due to illegal NgoMIV site in f1 origin. false Brooke Rothschild-Mancinelli component2441444 1 BBa_K314111 component2441446 1 BBa_K1319106 component2441454 1 BBa_K1615025 annotation2441454 1 BBa_K1615025 range2441454 1 1108 3777 annotation2441444 1 BBa_K314111 range2441444 1 1 1092 annotation2441446 1 BBa_K1319106 range2441446 1 1093 1107 BBa_B0105 1 Scar 25 RFC 25 Scar Sequence 2009-10-14T11:00:00Z 2015-08-31T04:07:21Z BBF RFC 25 This is the scar produced by assembly using RFC 25. If you are assembling a composite part using RFC 25, you can insert this part and specify blunt assembly to get the desired sequence. false false _1_ 0 25 397 Not in stock false Simple DNA sequence false Randy Rettberg annotation2041621 1 Scar 25 range2041621 1 1 6 BBa_K1321339 1 BBa_K1321339 CBDcenA+Linker, RFC 25 standard 2014-10-07T11:00:00Z 2015-06-17T12:14:18Z asdf CBDcenA in Freiburg format (RFC 25). false false _1696_ 4206 20830 9 In stock true asdf false Chris N Micklem annotation2418065 1 CBDCenA range2418065 1 1 318 annotation2414614 1 Linker: Pro-Thr box range2414614 1 319 408 BBa_K1319106 1 Scar25 ATG RFC 25 Translation Start Scar Sequence 2014-10-02T11:00:00Z 2015-05-08T01:09:51Z This sequence is produced by an assembly method. This sequence is produced by RFC10-assembly of a RFC10-RBS with a RFC25-prefixed part. false false _1694_ 0 19522 9 Not in stock false The resulting sequence is a combination of the RFC10 RBS-CDS scar (tactag) and 9 nucleotides from the RFC25 prefix (atg gcc ggc). false Michael Osthege annotation2393317 1 start range2393317 1 7 9 BBa_K1615035_sequence 1 gtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggacatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgatactagatggccggcgcaaatggtctgaaatttagtccgcgtaaagatgcactggcagttgcagttgtttgtgcatggcagagtccggtttttgcacatggtagcgaagcacatatggttccgctggataaaaccctgcaagaatttggtgcagatgttcagtgggatgattatgcacagatgtttaccctgattaaagatggtgcctacgttaaagttaaaccgggtgcaaaagatgccattgtgaatggtaaaagcctggatctgccggttccggttgttatgaaagaaggtaaagcctgggttagcgatacctttattaacgatgtttttcagagcggtctggatcagacctttcaggttgaaaaacgtccgcatccgctgaatagcctgagcgcagcagaaatttcaaaagcagttaccattgttaaagcagcccctgaatttcagccgaatacccgttttaccgaaattagcctgcatgaaccggataaagcagcagtttgggcatttgcactgcaaggcactccggttgatgcaccgcgtaccgcagatgttgttatgctggatggtaaacatgttattgaagccgttgtggacctgcaaaacaaaaaaatcctgagctggaccccgattaaaggtgcacataaaatggtgctgctggatgattttgttagcgtgcagaatattatcaacaccagcagcgaatttgccgaggttctgaaaaaacatggtattaccgatccgggtctggttgttaccacaccgctgaccgttggtttttttgatggtaaagatggcctgcaacaggatgcacgtctgctgaaagttgttagctatctggatacaggtgatggcaattattgggcacatccgattgaaaatctggttgccgttgttgatctggaagcgaaaaaaatcatcaaaatcgaagaaggtccggttattccggtgccgatggaaccgcgtccgtatgatggtcgtgatcgtaatgcaccggcagtgaaaccgctggaaattaccgaaccgaccggcaaaaactataccattacaggtgataccattcactggcagaattgggattttcatctgcgtctgaatagccgtgttggtccgattctgagcaccgttacctataatgataatggcaccaaacgtcaggtgatgtatgaaggtagcctgggtggtatgattgttccgtatggtgatccggatgttggttggtatttcaaagcatatctggatagcggtgattatggtatgggcaccctgaccagcccgattgttcgtggtaaagatgcaccgagcaatgcagttctgctggatgaaaccattgcagattataccggcaaaccgaccaccattccgggtgcagttgcaatttttgaacgttatgcaggtccggaatacaaacatctggaaatgggtaaaccgaatgttagcaccgaacgtcgtgaactggttgttcgttggattagcaccgttggtaactatgattatatcttcgattgggtgtttcacgataatgggaccattggtattgatgccggtgcaacaggtattgaagcagttaaaggtgttctggcaaaaaccatgcatgatccgagcgcaaaagaagatacccgttatggtacactgattgatcataatattgttggcaccacccatcagcacatttataactttcgtctggatctggatgttgatggcgaaaataacaccctggttgcaatggatgtggaagtgaaaccgaataccgcaggcggtccgcgtaccagcaccatgcaggttaatcagtatacaattgatagcgaacagaaagcagcccagaaatttgatccgggtacaattcgtctgctgagcaataccagcaaagaaaatcgtatgggtaatccggttagctatcagattatcccgtatgccggtggcacccatccggcagcaacaggtgcaaaatttgctccggatgaatggatttatcatcgtctgagctttatggataaacagctgtgggtgacccgttatcatccgaccgaacgttatccggaaggtaaatatccgaatcgtagcgcacatgatacaggtctgggtcagtatgcaaaagatgatgaaagcctgaccaatcatgatgatgttgtttggattaccacaggtacaacccatgttgcacgtgccgaagaatggccgattatgccgaccgaatgggcactggcactgctgaaaccgtggaacttttttgatgaaaccccgaccctgggtgagaaaaaaaaaaccggcgcgccgggttgccgtgttgactacgcggttaccaaccagtggccgggtggtttcggtgcgaacgttaccatcaccaacctgggtgacccggtttcttcttggaaactggactggacctacaccgcgggtcagcgtatccagcagctgtggaacggtaccgcgtctaccaacggtggtcaggtttctgttacctctctgccgtggaacggttctatcccgactggtggtaccgcgtctttcggtttcaacggttcttgggcgggttctaacccgaccccggcgtctttctctctgaacggtaccacctgcacgggtaccccgacgacctcgccaactcctacgccgaccccaaccacgccgactcctacaccgacgcccaccccaacgcctactccgacggtcaccccc BBa_B0105_sequence 1 accggc BBa_K1321339_sequence 1 gcgccgggttgccgtgttgactacgcggttaccaaccagtggccgggtggtttcggtgcgaacgttaccatcaccaacctgggtgacccggtttcttcttggaaactggactggacctacaccgcgggtcagcgtatccagcagctgtggaacggtaccgcgtctaccaacggtggtcaggtttctgttacctctctgccgtggaacggttctatcccgactggtggtaccgcgtctttcggtttcaacggttcttgggcgggttctaacccgaccccggcgtctttctctctgaacggtaccacctgcacgggtaccccgacgacctcgccaactcctacgccgaccccaaccacgccgactcctacaccgacgcccaccccaacgcctactccgacggtcaccccc BBa_K1615022_sequence 1 gcaaatggtctgaaatttagtccgcgtaaagatgcactggcagttgcagttgtttgtgcatggcagagtccggtttttgcacatggtagcgaagcacatatggttccgctggataaaaccctgcaagaatttggtgcagatgttcagtgggatgattatgcacagatgtttaccctgattaaagatggtgcctacgttaaagttaaaccgggtgcaaaagatgccattgtgaatggtaaaagcctggatctgccggttccggttgttatgaaagaaggtaaagcctgggttagcgatacctttattaacgatgtttttcagagcggtctggatcagacctttcaggttgaaaaacgtccgcatccgctgaatagcctgagcgcagcagaaatttcaaaagcagttaccattgttaaagcagcccctgaatttcagccgaatacccgttttaccgaaattagcctgcatgaaccggataaagcagcagtttgggcatttgcactgcaaggcactccggttgatgcaccgcgtaccgcagatgttgttatgctggatggtaaacatgttattgaagccgttgtggacctgcaaaacaaaaaaatcctgagctggaccccgattaaaggtgcacataaaatggtgctgctggatgattttgttagcgtgcagaatattatcaacaccagcagcgaatttgccgaggttctgaaaaaacatggtattaccgatccgggtctggttgttaccacaccgctgaccgttggtttttttgatggtaaagatggcctgcaacaggatgcacgtctgctgaaagttgttagctatctggatacaggtgatggcaattattgggcacatccgattgaaaatctggttgccgttgttgatctggaagcgaaaaaaatcatcaaaatcgaagaaggtccggttattccggtgccgatggaaccgcgtccgtatgatggtcgtgatcgtaatgcaccggcagtgaaaccgctggaaattaccgaaccgaccggcaaaaactataccattacaggtgataccattcactggcagaattgggattttcatctgcgtctgaatagccgtgttggtccgattctgagcaccgttacctataatgataatggcaccaaacgtcaggtgatgtatgaaggtagcctgggtggtatgattgttccgtatggtgatccggatgttggttggtatttcaaagcatatctggatagcggtgattatggtatgggcaccctgaccagcccgattgttcgtggtaaagatgcaccgagcaatgcagttctgctggatgaaaccattgcagattataccggcaaaccgaccaccattccgggtgcagttgcaatttttgaacgttatgcaggtccggaatacaaacatctggaaatgggtaaaccgaatgttagcaccgaacgtcgtgaactggttgttcgttggattagcaccgttggtaactatgattatatcttcgattgggtgtttcacgataatgggaccattggtattgatgccggtgcaacaggtattgaagcagttaaaggtgttctggcaaaaaccatgcatgatccgagcgcaaaagaagatacccgttatggtacactgattgatcataatattgttggcaccacccatcagcacatttataactttcgtctggatctggatgttgatggcgaaaataacaccctggttgcaatggatgtggaagtgaaaccgaataccgcaggcggtccgcgtaccagcaccatgcaggttaatcagtatacaattgatagcgaacagaaagcagcccagaaatttgatccgggtacaattcgtctgctgagcaataccagcaaagaaaatcgtatgggtaatccggttagctatcagattatcccgtatgccggtggcacccatccggcagcaacaggtgcaaaatttgctccggatgaatggatttatcatcgtctgagctttatggataaacagctgtgggtgacccgttatcatccgaccgaacgttatccggaaggtaaatatccgaatcgtagcgcacatgatacaggtctgggtcagtatgcaaaagatgatgaaagcctgaccaatcatgatgatgttgtttggattaccacaggtacaacccatgttgcacgtgccgaagaatggccgattatgccgaccgaatgggcactggcactgctgaaaccgtggaacttttttgatgaaaccccgaccctgggtgagaaaaaaaaa BBa_K1615025_sequence 1 gcaaatggtctgaaatttagtccgcgtaaagatgcactggcagttgcagttgtttgtgcatggcagagtccggtttttgcacatggtagcgaagcacatatggttccgctggataaaaccctgcaagaatttggtgcagatgttcagtgggatgattatgcacagatgtttaccctgattaaagatggtgcctacgttaaagttaaaccgggtgcaaaagatgccattgtgaatggtaaaagcctggatctgccggttccggttgttatgaaagaaggtaaagcctgggttagcgatacctttattaacgatgtttttcagagcggtctggatcagacctttcaggttgaaaaacgtccgcatccgctgaatagcctgagcgcagcagaaatttcaaaagcagttaccattgttaaagcagcccctgaatttcagccgaatacccgttttaccgaaattagcctgcatgaaccggataaagcagcagtttgggcatttgcactgcaaggcactccggttgatgcaccgcgtaccgcagatgttgttatgctggatggtaaacatgttattgaagccgttgtggacctgcaaaacaaaaaaatcctgagctggaccccgattaaaggtgcacataaaatggtgctgctggatgattttgttagcgtgcagaatattatcaacaccagcagcgaatttgccgaggttctgaaaaaacatggtattaccgatccgggtctggttgttaccacaccgctgaccgttggtttttttgatggtaaagatggcctgcaacaggatgcacgtctgctgaaagttgttagctatctggatacaggtgatggcaattattgggcacatccgattgaaaatctggttgccgttgttgatctggaagcgaaaaaaatcatcaaaatcgaagaaggtccggttattccggtgccgatggaaccgcgtccgtatgatggtcgtgatcgtaatgcaccggcagtgaaaccgctggaaattaccgaaccgaccggcaaaaactataccattacaggtgataccattcactggcagaattgggattttcatctgcgtctgaatagccgtgttggtccgattctgagcaccgttacctataatgataatggcaccaaacgtcaggtgatgtatgaaggtagcctgggtggtatgattgttccgtatggtgatccggatgttggttggtatttcaaagcatatctggatagcggtgattatggtatgggcaccctgaccagcccgattgttcgtggtaaagatgcaccgagcaatgcagttctgctggatgaaaccattgcagattataccggcaaaccgaccaccattccgggtgcagttgcaatttttgaacgttatgcaggtccggaatacaaacatctggaaatgggtaaaccgaatgttagcaccgaacgtcgtgaactggttgttcgttggattagcaccgttggtaactatgattatatcttcgattgggtgtttcacgataatgggaccattggtattgatgccggtgcaacaggtattgaagcagttaaaggtgttctggcaaaaaccatgcatgatccgagcgcaaaagaagatacccgttatggtacactgattgatcataatattgttggcaccacccatcagcacatttataactttcgtctggatctggatgttgatggcgaaaataacaccctggttgcaatggatgtggaagtgaaaccgaataccgcaggcggtccgcgtaccagcaccatgcaggttaatcagtatacaattgatagcgaacagaaagcagcccagaaatttgatccgggtacaattcgtctgctgagcaataccagcaaagaaaatcgtatgggtaatccggttagctatcagattatcccgtatgccggtggcacccatccggcagcaacaggtgcaaaatttgctccggatgaatggatttatcatcgtctgagctttatggataaacagctgtgggtgacccgttatcatccgaccgaacgttatccggaaggtaaatatccgaatcgtagcgcacatgatacaggtctgggtcagtatgcaaaagatgatgaaagcctgaccaatcatgatgatgttgtttggattaccacaggtacaacccatgttgcacgtgccgaagaatggccgattatgccgaccgaatgggcactggcactgctgaaaccgtggaacttttttgatgaaaccccgaccctgggtgagaaaaaaaaaaccggcgcgccgggttgccgtgttgactacgcggttaccaaccagtggccgggtggtttcggtgcgaacgttaccatcaccaacctgggtgacccggtttcttcttggaaactggactggacctacaccgcgggtcagcgtatccagcagctgtggaacggtaccgcgtctaccaacggtggtcaggtttctgttacctctctgccgtggaacggttctatcccgactggtggtaccgcgtctttcggtttcaacggttcttgggcgggttctaacccgaccccggcgtctttctctctgaacggtaccacctgcacgggtaccccgacgacctcgccaactcctacgccgaccccaaccacgccgactcctacaccgacgcccaccccaacgcctactccgacggtcaccccc BBa_K1319106_sequence 1 tactagatggccggc BBa_K314111_sequence 1 gtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggacatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z