LOCUS BBa_K1615036 4281 bp DNA linear 22-Nov-2024 DEFINITION Linker+dCBD fused to MaoA driven by LacI promoter ACCESSION BBa_K1615036 VERSION BBa_K1615036.1 FEATURES Location/Qualifiers misc_feature 18..324 /label=f1 origin misc_feature 1..455 /label=BBa_K314110 misc_feature 464..1555 /label=BBa_K314111 misc_feature 1566..1582 /label=lac O1 promoter 1583..1588 /label=-35 misc_feature 1589..1605 /label=lac O1 promoter 1606..1611 /label=-10 misc_feature 1564..1617 /label=BBa_R0011 promoter 1564..1617 /label=BBa_R0011 misc_feature 1631..1634 /label=conserved RBS 1627..1638 /label=BBa_B0034 misc_feature 1..1638 /label=BBa_K314103 misc_feature 1645..1647 /label=start misc_feature 1639..1653 /label=BBa_K1319106 CDS 1654..2019 /label=dCBD CDS 1654..1725 /label=Linker misc_feature 1726..1839 /label=cbh2 CBD CDS 1840..1911 /label=Linker misc_feature 1912..2019 /label=cbh1 CBD CDS 1654..2019 /label=BBa_K1321340 misc_feature 2020..2025 /label=Scar 25 misc_feature 2020..2025 /label=BBa_B0105 CDS 2026..4281 /label=MaoA CDS 2026..4281 /label=BBa_K1615022 misc_feature 1654..4281 /label=BBa_K1615026 ORIGIN 1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 421 acgcgaattt taacaaaata ttaacgttta caatttacta gaggtggtga atgtgaaacc 481 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt 541 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc 601 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct 661 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat 721 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg 781 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat 841 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt 901 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca 961 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc 1021 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa 1081 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat 1141 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct 1201 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg 1261 cgttggtgcg gacatctcgg tagtgggata cgacgatacc gaagacagct catgttatat 1321 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg 1381 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact 1441 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 1501 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgatacta 1561 gagaattgtg agcggataac aattgacatt gtgagcggat aacaagatac tgagcacata 1621 ctagagaaag aggagaaata ctagatggcc ggccccggcg ccaatccgcc cggtactacg 1681 accacttcgc gtccagcaac cacgaccggg tcgagccccg gcccacaggc ctgttcttcc 1741 gtctggggtc agtgcggcgg acagaactgg tctggcccta cctgctgtgc ttctggcagc 1801 acatgcgtct actctaacga ctactatagc cagtgcctgc cgggtgctaa cccgccaggc 1861 actaccacta cctctcgccc cgctaccact acaggctcta gcccgggacc tacccaaagc 1921 cactacggcc agtgcggagg cattggctac agcggcccta ccgtctgcgc ctccggaacc 1981 acctgccaag tcctgaaccc gtactactcc cagtgtctta ccggcgcaaa tggtctgaaa 2041 tttagtccgc gtaaagatgc actggcagtt gcagttgttt gtgcatggca gagtccggtt 2101 tttgcacatg gtagcgaagc acatatggtt ccgctggata aaaccctgca agaatttggt 2161 gcagatgttc agtgggatga ttatgcacag atgtttaccc tgattaaaga tggtgcctac 2221 gttaaagtta aaccgggtgc aaaagatgcc attgtgaatg gtaaaagcct ggatctgccg 2281 gttccggttg ttatgaaaga aggtaaagcc tgggttagcg atacctttat taacgatgtt 2341 tttcagagcg gtctggatca gacctttcag gttgaaaaac gtccgcatcc gctgaatagc 2401 ctgagcgcag cagaaatttc aaaagcagtt accattgtta aagcagcccc tgaatttcag 2461 ccgaataccc gttttaccga aattagcctg catgaaccgg ataaagcagc agtttgggca 2521 tttgcactgc aaggcactcc ggttgatgca ccgcgtaccg cagatgttgt tatgctggat 2581 ggtaaacatg ttattgaagc cgttgtggac ctgcaaaaca aaaaaatcct gagctggacc 2641 ccgattaaag gtgcacataa aatggtgctg ctggatgatt ttgttagcgt gcagaatatt 2701 atcaacacca gcagcgaatt tgccgaggtt ctgaaaaaac atggtattac cgatccgggt 2761 ctggttgtta ccacaccgct gaccgttggt ttttttgatg gtaaagatgg cctgcaacag 2821 gatgcacgtc tgctgaaagt tgttagctat ctggatacag gtgatggcaa ttattgggca 2881 catccgattg aaaatctggt tgccgttgtt gatctggaag cgaaaaaaat catcaaaatc 2941 gaagaaggtc cggttattcc ggtgccgatg gaaccgcgtc cgtatgatgg tcgtgatcgt 3001 aatgcaccgg cagtgaaacc gctggaaatt accgaaccga ccggcaaaaa ctataccatt 3061 acaggtgata ccattcactg gcagaattgg gattttcatc tgcgtctgaa tagccgtgtt 3121 ggtccgattc tgagcaccgt tacctataat gataatggca ccaaacgtca ggtgatgtat 3181 gaaggtagcc tgggtggtat gattgttccg tatggtgatc cggatgttgg ttggtatttc 3241 aaagcatatc tggatagcgg tgattatggt atgggcaccc tgaccagccc gattgttcgt 3301 ggtaaagatg caccgagcaa tgcagttctg ctggatgaaa ccattgcaga ttataccggc 3361 aaaccgacca ccattccggg tgcagttgca atttttgaac gttatgcagg tccggaatac 3421 aaacatctgg aaatgggtaa accgaatgtt agcaccgaac gtcgtgaact ggttgttcgt 3481 tggattagca ccgttggtaa ctatgattat atcttcgatt gggtgtttca cgataatggg 3541 accattggta ttgatgccgg tgcaacaggt attgaagcag ttaaaggtgt tctggcaaaa 3601 accatgcatg atccgagcgc aaaagaagat acccgttatg gtacactgat tgatcataat 3661 attgttggca ccacccatca gcacatttat aactttcgtc tggatctgga tgttgatggc 3721 gaaaataaca ccctggttgc aatggatgtg gaagtgaaac cgaataccgc aggcggtccg 3781 cgtaccagca ccatgcaggt taatcagtat acaattgata gcgaacagaa agcagcccag 3841 aaatttgatc cgggtacaat tcgtctgctg agcaatacca gcaaagaaaa tcgtatgggt 3901 aatccggtta gctatcagat tatcccgtat gccggtggca cccatccggc agcaacaggt 3961 gcaaaatttg ctccggatga atggatttat catcgtctga gctttatgga taaacagctg 4021 tgggtgaccc gttatcatcc gaccgaacgt tatccggaag gtaaatatcc gaatcgtagc 4081 gcacatgata caggtctggg tcagtatgca aaagatgatg aaagcctgac caatcatgat 4141 gatgttgttt ggattaccac aggtacaacc catgttgcac gtgccgaaga atggccgatt 4201 atgccgaccg aatgggcact ggcactgctg aaaccgtgga acttttttga tgaaaccccg 4261 accctgggtg agaaaaaaaa a //