LOCUS BBa_K1615039 4242 bp DNA linear 22-Nov-2024 DEFINITION MaoA fused to CBDcex driven by LacI promoter ACCESSION BBa_K1615039 VERSION BBa_K1615039.1 FEATURES Location/Qualifiers misc_feature 18..324 /label=f1 origin misc_feature 1..455 /label=BBa_K314110 misc_feature 464..1555 /label=BBa_K314111 misc_feature 1566..1582 /label=lac O1 promoter 1583..1588 /label=-35 misc_feature 1589..1605 /label=lac O1 promoter 1606..1611 /label=-10 misc_feature 1564..1617 /label=BBa_R0011 promoter 1564..1617 /label=BBa_R0011 misc_feature 1631..1634 /label=conserved RBS 1627..1638 /label=BBa_B0034 misc_feature 1..1638 /label=BBa_K314103 misc_feature 1645..1647 /label=start misc_feature 1639..1653 /label=BBa_K1319106 CDS 1654..3909 /label=MaoA CDS 1654..3909 /label=BBa_K1615022 misc_feature 3910..3915 /label=Scar 25 misc_feature 3910..3915 /label=BBa_B0105 CDS 3916..4242 /label=BBa_K1321003 misc_feature 1654..4242 /label=BBa_K1615029 ORIGIN 1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 421 acgcgaattt taacaaaata ttaacgttta caatttacta gaggtggtga atgtgaaacc 481 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt 541 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc 601 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct 661 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat 721 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg 781 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat 841 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt 901 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca 961 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc 1021 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa 1081 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat 1141 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct 1201 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg 1261 cgttggtgcg gacatctcgg tagtgggata cgacgatacc gaagacagct catgttatat 1321 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg 1381 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact 1441 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 1501 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgatacta 1561 gagaattgtg agcggataac aattgacatt gtgagcggat aacaagatac tgagcacata 1621 ctagagaaag aggagaaata ctagatggcc ggcgcaaatg gtctgaaatt tagtccgcgt 1681 aaagatgcac tggcagttgc agttgtttgt gcatggcaga gtccggtttt tgcacatggt 1741 agcgaagcac atatggttcc gctggataaa accctgcaag aatttggtgc agatgttcag 1801 tgggatgatt atgcacagat gtttaccctg attaaagatg gtgcctacgt taaagttaaa 1861 ccgggtgcaa aagatgccat tgtgaatggt aaaagcctgg atctgccggt tccggttgtt 1921 atgaaagaag gtaaagcctg ggttagcgat acctttatta acgatgtttt tcagagcggt 1981 ctggatcaga cctttcaggt tgaaaaacgt ccgcatccgc tgaatagcct gagcgcagca 2041 gaaatttcaa aagcagttac cattgttaaa gcagcccctg aatttcagcc gaatacccgt 2101 tttaccgaaa ttagcctgca tgaaccggat aaagcagcag tttgggcatt tgcactgcaa 2161 ggcactccgg ttgatgcacc gcgtaccgca gatgttgtta tgctggatgg taaacatgtt 2221 attgaagccg ttgtggacct gcaaaacaaa aaaatcctga gctggacccc gattaaaggt 2281 gcacataaaa tggtgctgct ggatgatttt gttagcgtgc agaatattat caacaccagc 2341 agcgaatttg ccgaggttct gaaaaaacat ggtattaccg atccgggtct ggttgttacc 2401 acaccgctga ccgttggttt ttttgatggt aaagatggcc tgcaacagga tgcacgtctg 2461 ctgaaagttg ttagctatct ggatacaggt gatggcaatt attgggcaca tccgattgaa 2521 aatctggttg ccgttgttga tctggaagcg aaaaaaatca tcaaaatcga agaaggtccg 2581 gttattccgg tgccgatgga accgcgtccg tatgatggtc gtgatcgtaa tgcaccggca 2641 gtgaaaccgc tggaaattac cgaaccgacc ggcaaaaact ataccattac aggtgatacc 2701 attcactggc agaattggga ttttcatctg cgtctgaata gccgtgttgg tccgattctg 2761 agcaccgtta cctataatga taatggcacc aaacgtcagg tgatgtatga aggtagcctg 2821 ggtggtatga ttgttccgta tggtgatccg gatgttggtt ggtatttcaa agcatatctg 2881 gatagcggtg attatggtat gggcaccctg accagcccga ttgttcgtgg taaagatgca 2941 ccgagcaatg cagttctgct ggatgaaacc attgcagatt ataccggcaa accgaccacc 3001 attccgggtg cagttgcaat ttttgaacgt tatgcaggtc cggaatacaa acatctggaa 3061 atgggtaaac cgaatgttag caccgaacgt cgtgaactgg ttgttcgttg gattagcacc 3121 gttggtaact atgattatat cttcgattgg gtgtttcacg ataatgggac cattggtatt 3181 gatgccggtg caacaggtat tgaagcagtt aaaggtgttc tggcaaaaac catgcatgat 3241 ccgagcgcaa aagaagatac ccgttatggt acactgattg atcataatat tgttggcacc 3301 acccatcagc acatttataa ctttcgtctg gatctggatg ttgatggcga aaataacacc 3361 ctggttgcaa tggatgtgga agtgaaaccg aataccgcag gcggtccgcg taccagcacc 3421 atgcaggtta atcagtatac aattgatagc gaacagaaag cagcccagaa atttgatccg 3481 ggtacaattc gtctgctgag caataccagc aaagaaaatc gtatgggtaa tccggttagc 3541 tatcagatta tcccgtatgc cggtggcacc catccggcag caacaggtgc aaaatttgct 3601 ccggatgaat ggatttatca tcgtctgagc tttatggata aacagctgtg ggtgacccgt 3661 tatcatccga ccgaacgtta tccggaaggt aaatatccga atcgtagcgc acatgataca 3721 ggtctgggtc agtatgcaaa agatgatgaa agcctgacca atcatgatga tgttgtttgg 3781 attaccacag gtacaaccca tgttgcacgt gccgaagaat ggccgattat gccgaccgaa 3841 tgggcactgg cactgctgaa accgtggaac ttttttgatg aaaccccgac cctgggtgag 3901 aaaaaaaaaa ccggcggtcc ggccgggtgc caggtgctgt ggggcgtcaa ccagtggaac 3961 accggcttca ccgcgaacgt caccgtgaag aacacgtcct ccgctccggt agacggctgg 4021 acgctcacgt tcagcttccc gtccggccag caggtcaccc aggcgtggag ctcgacggtc 4081 acgcagtccg gctcggccgt gacggtccgc aacgccccgt ggaacggctc gatcccggcg 4141 ggcggcaccg cgcagttcgg cttcaacggc tcgcacacgg gcaccaacgc cgcgccgacg 4201 gcgttctcgc tcaacggcac gccctgcacg gtcggcggca gc //