LOCUS BBa_K1615042 4626 bp DNA linear 26-Nov-2024 DEFINITION Linker-CBDcipA-linker fused to MaoA driven by LacI promoter ACCESSION BBa_K1615042 VERSION BBa_K1615042.1 FEATURES Location/Qualifiers misc_feature 18..324 /label=f1 origin misc_feature 1..455 /label=BBa_K314110 misc_feature 464..1555 /label=BBa_K314111 misc_feature 1566..1582 /label=lac O1 promoter 1583..1588 /label=-35 misc_feature 1589..1605 /label=lac O1 promoter 1606..1611 /label=-10 misc_feature 1564..1617 /label=BBa_R0011 promoter 1564..1617 /label=BBa_R0011 misc_feature 1631..1634 /label=conserved RBS 1627..1638 /label=BBa_B0034 misc_feature 1..1638 /label=BBa_K314103 misc_feature 1645..1647 /label=start misc_feature 1639..1653 /label=BBa_K1319106 CDS 1654..2364 /label=BBa_K1615111 misc_feature 2365..2370 /label=Scar 25 misc_feature 2365..2370 /label=BBa_B0105 CDS 2371..4626 /label=MaoA CDS 2371..4626 /label=BBa_K1615022 misc_feature 1654..4626 /label=BBa_K1615032 ORIGIN 1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 421 acgcgaattt taacaaaata ttaacgttta caatttacta gaggtggtga atgtgaaacc 481 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt 541 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc 601 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct 661 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat 721 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg 781 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat 841 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt 901 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca 961 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc 1021 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa 1081 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat 1141 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct 1201 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg 1261 cgttggtgcg gacatctcgg tagtgggata cgacgatacc gaagacagct catgttatat 1321 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg 1381 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact 1441 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 1501 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgatacta 1561 gagaattgtg agcggataac aattgacatt gtgagcggat aacaagatac tgagcacata 1621 ctagagaaag aggagaaata ctagatggcc ggcaatgcaa ccccgaccaa aggtgccacc 1681 ccgacaaata ccgccacacc gaccaaaagc gcaaccgcga ccccgacccg tccgagcgtt 1741 ccgaccaata cccctaccaa tacaccggca aatactccgg ttagcggtaa tctgaaagtg 1801 gaattttaca atagcaatcc gagcgatacc accaatagca ttaatccgca gtttaaagtt 1861 accaataccg gcagcagcgc aattgatctg agcaaactga ccctgcgtta ttactatacc 1921 gttgatggtc agaaagatca gacattttgg tgtgatcatg cagccattat tggtagcaat 1981 ggtagctata atggcattac cagcaatgtg aaaggcacct tcgttaaaat gagcagcagc 2041 acaaataatg ccgataccta tctggaaatt agctttacag gtggcaccct ggaaccgggt 2101 gcacatgttc agattcaggg tcgttttgcc aaaaatgatt ggagcaatta tacccagagc 2161 aacgactata gctttaaaag cgccagccag tttgttgaat gggatcaggt taccgcatat 2221 ctgaatggtg ttctggtttg gggtaaagaa cctggtggta gcgttgttcc gagcacccag 2281 ccggttacca cccctccggc aaccaccaaa ccgcctgcca ccacaaaacc accggcaaca 2341 accattccgc ctagtgatga tccgaccggc gcaaatggtc tgaaatttag tccgcgtaaa 2401 gatgcactgg cagttgcagt tgtttgtgca tggcagagtc cggtttttgc acatggtagc 2461 gaagcacata tggttccgct ggataaaacc ctgcaagaat ttggtgcaga tgttcagtgg 2521 gatgattatg cacagatgtt taccctgatt aaagatggtg cctacgttaa agttaaaccg 2581 ggtgcaaaag atgccattgt gaatggtaaa agcctggatc tgccggttcc ggttgttatg 2641 aaagaaggta aagcctgggt tagcgatacc tttattaacg atgtttttca gagcggtctg 2701 gatcagacct ttcaggttga aaaacgtccg catccgctga atagcctgag cgcagcagaa 2761 atttcaaaag cagttaccat tgttaaagca gcccctgaat ttcagccgaa tacccgtttt 2821 accgaaatta gcctgcatga accggataaa gcagcagttt gggcatttgc actgcaaggc 2881 actccggttg atgcaccgcg taccgcagat gttgttatgc tggatggtaa acatgttatt 2941 gaagccgttg tggacctgca aaacaaaaaa atcctgagct ggaccccgat taaaggtgca 3001 cataaaatgg tgctgctgga tgattttgtt agcgtgcaga atattatcaa caccagcagc 3061 gaatttgccg aggttctgaa aaaacatggt attaccgatc cgggtctggt tgttaccaca 3121 ccgctgaccg ttggtttttt tgatggtaaa gatggcctgc aacaggatgc acgtctgctg 3181 aaagttgtta gctatctgga tacaggtgat ggcaattatt gggcacatcc gattgaaaat 3241 ctggttgccg ttgttgatct ggaagcgaaa aaaatcatca aaatcgaaga aggtccggtt 3301 attccggtgc cgatggaacc gcgtccgtat gatggtcgtg atcgtaatgc accggcagtg 3361 aaaccgctgg aaattaccga accgaccggc aaaaactata ccattacagg tgataccatt 3421 cactggcaga attgggattt tcatctgcgt ctgaatagcc gtgttggtcc gattctgagc 3481 accgttacct ataatgataa tggcaccaaa cgtcaggtga tgtatgaagg tagcctgggt 3541 ggtatgattg ttccgtatgg tgatccggat gttggttggt atttcaaagc atatctggat 3601 agcggtgatt atggtatggg caccctgacc agcccgattg ttcgtggtaa agatgcaccg 3661 agcaatgcag ttctgctgga tgaaaccatt gcagattata ccggcaaacc gaccaccatt 3721 ccgggtgcag ttgcaatttt tgaacgttat gcaggtccgg aatacaaaca tctggaaatg 3781 ggtaaaccga atgttagcac cgaacgtcgt gaactggttg ttcgttggat tagcaccgtt 3841 ggtaactatg attatatctt cgattgggtg tttcacgata atgggaccat tggtattgat 3901 gccggtgcaa caggtattga agcagttaaa ggtgttctgg caaaaaccat gcatgatccg 3961 agcgcaaaag aagatacccg ttatggtaca ctgattgatc ataatattgt tggcaccacc 4021 catcagcaca tttataactt tcgtctggat ctggatgttg atggcgaaaa taacaccctg 4081 gttgcaatgg atgtggaagt gaaaccgaat accgcaggcg gtccgcgtac cagcaccatg 4141 caggttaatc agtatacaat tgatagcgaa cagaaagcag cccagaaatt tgatccgggt 4201 acaattcgtc tgctgagcaa taccagcaaa gaaaatcgta tgggtaatcc ggttagctat 4261 cagattatcc cgtatgccgg tggcacccat ccggcagcaa caggtgcaaa atttgctccg 4321 gatgaatgga tttatcatcg tctgagcttt atggataaac agctgtgggt gacccgttat 4381 catccgaccg aacgttatcc ggaaggtaaa tatccgaatc gtagcgcaca tgatacaggt 4441 ctgggtcagt atgcaaaaga tgatgaaagc ctgaccaatc atgatgatgt tgtttggatt 4501 accacaggta caacccatgt tgcacgtgcc gaagaatggc cgattatgcc gaccgaatgg 4561 gcactggcac tgctgaaacc gtggaacttt tttgatgaaa ccccgaccct gggtgagaaa 4621 aaaaaa //