LOCUS BBa_K1615046 2622 bp DNA linear 22-Nov-2024 DEFINITION Heroin Esterase driven by LacI promoter ACCESSION BBa_K1615046 VERSION BBa_K1615046.1 FEATURES Location/Qualifiers misc_feature 18..324 /label=f1 origin misc_feature 1..455 /label=BBa_K314110 misc_feature 464..1555 /label=BBa_K314111 misc_feature 1566..1582 /label=lac O1 promoter 1583..1588 /label=-35 misc_feature 1589..1605 /label=lac O1 promoter 1606..1611 /label=-10 misc_feature 1564..1617 /label=BBa_R0011 promoter 1564..1617 /label=BBa_R0011 misc_feature 1631..1634 /label=conserved RBS 1627..1638 /label=BBa_B0034 misc_feature 1..1638 /label=BBa_K314103 misc_feature 1645..1647 /label=start misc_feature 1639..1653 /label=BBa_K1319106 CDS 1654..2622 /label=BBa_K1615045 ORIGIN 1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 421 acgcgaattt taacaaaata ttaacgttta caatttacta gaggtggtga atgtgaaacc 481 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt 541 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc 601 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct 661 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat 721 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg 781 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat 841 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt 901 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca 961 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc 1021 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa 1081 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat 1141 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct 1201 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg 1261 cgttggtgcg gacatctcgg tagtgggata cgacgatacc gaagacagct catgttatat 1321 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg 1381 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact 1441 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 1501 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgatacta 1561 gagaattgtg agcggataac aattgacatt gtgagcggat aacaagatac tgagcacata 1621 ctagagaaag aggagaaata ctagatggcc ggcatgacca cctttccgac cctggatccg 1681 gaactggcag cagcactgac catgctgccg aaagttgatt ttgccgatct gccgaatgca 1741 cgtgccacct ataatgcact gattggtgca atgctggcgg atctgccgtt tgatggtgtt 1801 agcctgcgtg aactgagcgc accgggtctg gatggtgatc cggaagttac cattcgtttt 1861 tttacaccgg ataataccgc aggtccggtt ccggttctgc tgtggattca tggtggtggt 1921 tttgcaattg gcaccgcaga aagcagcgat ccgttttgtg ttgaagttgc acgtgaactg 1981 ggttttgcag ttgcaaatgt tgaatatcgt ctggcaccgg aaaccagctt tccgggtccg 2041 gttaatgatt gttatgcagc actgctgtat attcatgcac atgccgaaga actgggtgtt 2101 gatccgagcc gtattgcagt tggtggtcag agtgccggtg gtggtctggc agccggtaca 2161 gttctgaaag cacgtgatga aggtgttgtt ccggttgcat ttcagtttct ggaaattcct 2221 gaactggatg atcgcctggc aaccgttagc atgaccaatt ttgttgatac accgctgtgg 2281 catcgtccga atgcaattct gagctggaaa tattacctgg gtgaaagcta tagcggtccg 2341 gaagatccgg atgtgagcat ttatgccgca ccgagccgtg caaccgatct gacaggtctg 2401 cctccgacct atctgagcac catggaactg gatccgctgc gtgatgaggg tctggaatat 2461 gcactgcgtc tgctgcatgc cggtgttagc gttgaactgc atagttttcc gggtacattt 2521 catggtagcg cactggttgc caccgcagca gttagcgaac gtggtgcagc agaagccctg 2581 acagccctgc gtcgtggtct gcgtagcctg agtccggtta gc //