LOCUS       BBa_K1615058            3036 bp    DNA     linear       22-Nov-2024
DEFINITION  Heroin esterase fused to CBDcenA+Linker in RFC25 driven by LacI 
            promoter
ACCESSION   BBa_K1615058
VERSION     BBa_K1615058.1  
FEATURES             Location/Qualifiers
     misc_feature    18..324
                     /label=f1 origin
     misc_feature    1..455
                     /label=BBa_K314110
     misc_feature    464..1555
                     /label=BBa_K314111
     misc_feature    1566..1582
                     /label=lac O1
     promoter        1583..1588
                     /label=-35
     misc_feature    1589..1605
                     /label=lac O1
     promoter        1606..1611
                     /label=-10
     misc_feature    1564..1617
                     /label=BBa_R0011
     promoter        1564..1617
                     /label=BBa_R0011
     misc_feature    1631..1634
                     /label=conserved
     RBS             1627..1638
                     /label=BBa_B0034
     misc_feature    1..1638
                     /label=BBa_K314103
     misc_feature    1639..1644
                     /label=Scar 25
     misc_feature    1639..1644
                     /label=BBa_B0105
     CDS             1645..2613
                     /label=BBa_K1615045
     misc_feature    2620..2622
                     /label=start
     misc_feature    2614..2628
                     /label=BBa_K1319106
     CDS             2947..3036
                     /label=Linker: Pro-Thr box
     CDS             2629..2946
                     /label=CBDCenA
     CDS             2629..3036
                     /label=BBa_K1321339
     misc_feature    1645..3036
                     /label=BBa_K1615048
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgttta caatttacta gaggtggtga atgtgaaacc
      481 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
      541 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
      601 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
      661 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
      721 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
      781 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
      841 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
      901 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
      961 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
     1021 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
     1081 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
     1141 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
     1201 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
     1261 cgttggtgcg gacatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
     1321 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
     1381 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
     1441 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     1501 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgatacta
     1561 gagaattgtg agcggataac aattgacatt gtgagcggat aacaagatac tgagcacata
     1621 ctagagaaag aggagaaaac cggcatgacc acctttccga ccctggatcc ggaactggca
     1681 gcagcactga ccatgctgcc gaaagttgat tttgccgatc tgccgaatgc acgtgccacc
     1741 tataatgcac tgattggtgc aatgctggcg gatctgccgt ttgatggtgt tagcctgcgt
     1801 gaactgagcg caccgggtct ggatggtgat ccggaagtta ccattcgttt ttttacaccg
     1861 gataataccg caggtccggt tccggttctg ctgtggattc atggtggtgg ttttgcaatt
     1921 ggcaccgcag aaagcagcga tccgttttgt gttgaagttg cacgtgaact gggttttgca
     1981 gttgcaaatg ttgaatatcg tctggcaccg gaaaccagct ttccgggtcc ggttaatgat
     2041 tgttatgcag cactgctgta tattcatgca catgccgaag aactgggtgt tgatccgagc
     2101 cgtattgcag ttggtggtca gagtgccggt ggtggtctgg cagccggtac agttctgaaa
     2161 gcacgtgatg aaggtgttgt tccggttgca tttcagtttc tggaaattcc tgaactggat
     2221 gatcgcctgg caaccgttag catgaccaat tttgttgata caccgctgtg gcatcgtccg
     2281 aatgcaattc tgagctggaa atattacctg ggtgaaagct atagcggtcc ggaagatccg
     2341 gatgtgagca tttatgccgc accgagccgt gcaaccgatc tgacaggtct gcctccgacc
     2401 tatctgagca ccatggaact ggatccgctg cgtgatgagg gtctggaata tgcactgcgt
     2461 ctgctgcatg ccggtgttag cgttgaactg catagttttc cgggtacatt tcatggtagc
     2521 gcactggttg ccaccgcagc agttagcgaa cgtggtgcag cagaagccct gacagccctg
     2581 cgtcgtggtc tgcgtagcct gagtccggtt agctactaga tggccggcgc gccgggttgc
     2641 cgtgttgact acgcggttac caaccagtgg ccgggtggtt tcggtgcgaa cgttaccatc
     2701 accaacctgg gtgacccggt ttcttcttgg aaactggact ggacctacac cgcgggtcag
     2761 cgtatccagc agctgtggaa cggtaccgcg tctaccaacg gtggtcaggt ttctgttacc
     2821 tctctgccgt ggaacggttc tatcccgact ggtggtaccg cgtctttcgg tttcaacggt
     2881 tcttgggcgg gttctaaccc gaccccggcg tctttctctc tgaacggtac cacctgcacg
     2941 ggtaccccga cgacctcgcc aactcctacg ccgaccccaa ccacgccgac tcctacaccg
     3001 acgcccaccc caacgcctac tccgacggtc accccc
//