LOCUS       BBa_K1615059            3003 bp    DNA     linear       07-May-2024
DEFINITION  Linker+dCBD fused to Heroin Esterase driven by LacI promoter
ACCESSION   BBa_K1615059
VERSION     BBa_K1615059.1  
FEATURES             Location/Qualifiers
     misc_feature    18..324
                     /label=f1 origin
     misc_feature    1..455
                     /label=BBa_K314110
     misc_feature    464..1555
                     /label=BBa_K314111
     misc_feature    1566..1582
                     /label=lac O1
     promoter        1583..1588
                     /label=-35
     misc_feature    1589..1605
                     /label=lac O1
     promoter        1606..1611
                     /label=-10
     misc_feature    1564..1617
                     /label=BBa_R0011
     promoter        1564..1617
                     /label=BBa_R0011
     misc_feature    1631..1634
                     /label=conserved
     RBS             1627..1638
                     /label=BBa_B0034
     misc_feature    1..1638
                     /label=BBa_K314103
     misc_feature    1645..1647
                     /label=start
     misc_feature    1639..1653
                     /label=BBa_K1319106
     CDS             1654..2019
                     /label=dCBD
     CDS             1654..1725
                     /label=Linker
     misc_feature    1726..1839
                     /label=cbh2 CBD
     CDS             1840..1911
                     /label=Linker
     misc_feature    1912..2019
                     /label=cbh1 CBD
     CDS             1654..2019
                     /label=BBa_K1321340
     misc_feature    2026..2028
                     /label=start
     misc_feature    2020..2034
                     /label=BBa_K1319106
     CDS             2035..3003
                     /label=BBa_K1615045
     misc_feature    1654..3003
                     /label=BBa_K1615049
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgttta caatttacta gaggtggtga atgtgaaacc
      481 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
      541 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
      601 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
      661 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
      721 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
      781 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
      841 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
      901 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
      961 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
     1021 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
     1081 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
     1141 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
     1201 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
     1261 cgttggtgcg gacatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
     1321 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
     1381 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
     1441 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     1501 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgatacta
     1561 gagaattgtg agcggataac aattgacatt gtgagcggat aacaagatac tgagcacata
     1621 ctagagaaag aggagaaata ctagatggcc ggccccggcg ccaatccgcc cggtactacg
     1681 accacttcgc gtccagcaac cacgaccggg tcgagccccg gcccacaggc ctgttcttcc
     1741 gtctggggtc agtgcggcgg acagaactgg tctggcccta cctgctgtgc ttctggcagc
     1801 acatgcgtct actctaacga ctactatagc cagtgcctgc cgggtgctaa cccgccaggc
     1861 actaccacta cctctcgccc cgctaccact acaggctcta gcccgggacc tacccaaagc
     1921 cactacggcc agtgcggagg cattggctac agcggcccta ccgtctgcgc ctccggaacc
     1981 acctgccaag tcctgaaccc gtactactcc cagtgtcttt actagatggc cggcatgacc
     2041 acctttccga ccctggatcc ggaactggca gcagcactga ccatgctgcc gaaagttgat
     2101 tttgccgatc tgccgaatgc acgtgccacc tataatgcac tgattggtgc aatgctggcg
     2161 gatctgccgt ttgatggtgt tagcctgcgt gaactgagcg caccgggtct ggatggtgat
     2221 ccggaagtta ccattcgttt ttttacaccg gataataccg caggtccggt tccggttctg
     2281 ctgtggattc atggtggtgg ttttgcaatt ggcaccgcag aaagcagcga tccgttttgt
     2341 gttgaagttg cacgtgaact gggttttgca gttgcaaatg ttgaatatcg tctggcaccg
     2401 gaaaccagct ttccgggtcc ggttaatgat tgttatgcag cactgctgta tattcatgca
     2461 catgccgaag aactgggtgt tgatccgagc cgtattgcag ttggtggtca gagtgccggt
     2521 ggtggtctgg cagccggtac agttctgaaa gcacgtgatg aaggtgttgt tccggttgca
     2581 tttcagtttc tggaaattcc tgaactggat gatcgcctgg caaccgttag catgaccaat
     2641 tttgttgata caccgctgtg gcatcgtccg aatgcaattc tgagctggaa atattacctg
     2701 ggtgaaagct atagcggtcc ggaagatccg gatgtgagca tttatgccgc accgagccgt
     2761 gcaaccgatc tgacaggtct gcctccgacc tatctgagca ccatggaact ggatccgctg
     2821 cgtgatgagg gtctggaata tgcactgcgt ctgctgcatg ccggtgttag cgttgaactg
     2881 catagttttc cgggtacatt tcatggtagc gcactggttg ccaccgcagc agttagcgaa
     2941 cgtggtgcag cagaagccct gacagccctg cgtcgtggtc tgcgtagcct gagtccggtt
     3001 agc
//