BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K314111 1 BBa_K314111 Lac I 2010-10-07T11:00:00Z 2015-05-08T01:11:55Z Pet 29 vector Lac I gene with promoter and RBS false false _496_ 0 6395 9 It's complicated false false IGEM 2010 Team Washington BBa_K314103 1 BBa_K314103 Lac induced expression cassette 2010-10-07T11:00:00Z 2015-05-08T01:11:55Z construct Released HQ 2013 Lac induced protein expression insert includes f1 origin, Lac I gene and RBS false false _496_ 0 6395 9 In stock true false Tomas Huber, Justin Siegel component2091083 1 BBa_R0011 component2091089 1 BBa_B0034 component2091082 1 BBa_K314111 component2091081 1 BBa_K314110 annotation2091082 1 BBa_K314111 range2091082 1 464 1555 annotation2091089 1 BBa_B0034 range2091089 1 1627 1638 annotation2091081 1 BBa_K314110 range2091081 1 1 455 annotation2091083 1 BBa_R0011 range2091083 1 1564 1617 BBa_K314110 1 f1 ori f1 origin 2010-10-07T11:00:00Z 2015-05-08T01:11:55Z Pet 29 vector Released HQ 2013 Phage origin recognized by M13. Used to make SS DNA when infected with M13k07 helper phage false false _496_ 0 6395 9 In stock true false IGEM 2010 Team Washington annotation2115768 1 f1 origin range2115768 1 18 324 BBa_K1615045 1 HerE Heroin Esterase in RFC25 2015-08-27T11:00:00Z 2015-08-28T08:44:34Z Heroin Esterase from Rhodococcus erythropolis. Heroin Esterase. Converts Heroin to Morphine. false false _2032_ 25547 25547 9 false Codon optimised for E. coli. RFC 25 compatible. false Brooke Rothschild-Mancinelli BBa_R0011 1 lacI+pL Promoter (lacI regulated, lambda pL hybrid) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z represillator of Elowitz and Leibler (2000) Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference). false true _1_ 0 24 7 In stock false <P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs. true Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton annotation1999 1 lac O1 range1999 1 3 19 annotation2002 1 -10 range2002 1 43 48 annotation7064 1 BBa_R0011 range7064 1 1 54 annotation2000 1 -35 range2000 1 20 25 annotation2001 1 lac O1 range2001 1 26 42 BBa_K1615062 1 BBa_K1615062 HerE fused to CBDcex driven by LacI promoter 2015-08-27T11:00:00Z 2015-08-28T09:03:30Z Heroin Esterase from Rhodococcus erythropolis. CBDcex from Cellulomonas fimi. Lac expression cassette from iGEM10_Washington. Heroin Esterase fused N terminally to CBDcex driven by the IPTG inducible PLlac 0-1 promoter. false false _2032_ 25547 25547 9 false Fusion was performed using RFC10 standard due to illegal NgoMIV site in f1 origin. false Brooke Rothschild-Mancinelli component2439577 1 BBa_K1615052 component2439572 1 BBa_K1319106 component2439570 1 BBa_K314103 annotation2439570 1 BBa_K314103 range2439570 1 1 1638 annotation2439572 1 BBa_K1319106 range2439572 1 1639 1653 annotation2439577 1 BBa_K1615052 range2439577 1 1654 2955 BBa_B0105 1 Scar 25 RFC 25 Scar Sequence 2009-10-14T11:00:00Z 2015-08-31T04:07:21Z BBF RFC 25 This is the scar produced by assembly using RFC 25. If you are assembling a composite part using RFC 25, you can insert this part and specify blunt assembly to get the desired sequence. false false _1_ 0 25 397 Not in stock false Simple DNA sequence false Randy Rettberg annotation2041621 1 Scar 25 range2041621 1 1 6 BBa_K1615052 1 BBa_K1615052 HerE fused to CBDcex in RFC25 2015-08-27T11:00:00Z 2015-08-28T08:52:43Z Heroin Esterase from Rhodococcus erythropolis. CBDcex from Cellulomonas fimi. Heroin Esterase fused N terminally to CBDcex. false false _2032_ 25547 25547 9 false Fusion done using RFC25. false Brooke Rothschild-Mancinelli component2439531 1 BBa_K1615045 component2439533 1 BBa_B0105 component2439534 1 BBa_K1321003 annotation2439533 1 BBa_B0105 range2439533 1 970 975 annotation2439531 1 BBa_K1615045 range2439531 1 1 969 annotation2439534 1 BBa_K1321003 range2439534 1 976 1302 BBa_K1319106 1 Scar25 ATG RFC 25 Translation Start Scar Sequence 2014-10-02T11:00:00Z 2015-05-08T01:09:51Z This sequence is produced by an assembly method. This sequence is produced by RFC10-assembly of a RFC10-RBS with a RFC25-prefixed part. false false _1694_ 0 19522 9 Not in stock false The resulting sequence is a combination of the RFC10 RBS-CDS scar (tactag) and 9 nucleotides from the RFC25 prefix (atg gcc ggc). false Michael Osthege annotation2393317 1 start range2393317 1 7 9 BBa_K1321003 1 BBa_K1321003 Re-entry of BBa_K863101, Cellulose Binding Domain CBDcex in rfc25 2014-10-09T11:00:00Z 2015-06-17T12:35:33Z This is a direct copy of BBa_K863101 with slight modifications to the beginning and end of the sequence for the purposes of allowing composite parts to be added correctly using this part. This part is a copy of BBa_K863101 (Cellulose binding Domain of Cellulomonas Fimi Exoglucanse (Freiburg-Standard)) and was created only as a registry entry, removing the atg-NgoMIV parts at the front and the AgeI-taa basepairs from the sequence so that the registry does not flag it as rfc25 incompatible, and it enables us to enter fusion proteins with this part as automatic composite parts in the method as described by: http://2009.igem.org/Team:Freiburg_bioware/general. false false _1696_ 4206 20780 9 Not in stock false This is a direct copy of BBa_K863101 with slight modifications to the beginning and end of the sequence for the purposes of allowing composite parts to be added correctly using this part. false Xenia Spencer-Milnes BBa_K314110_sequence 1 acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatt BBa_B0105_sequence 1 accggc BBa_K1321003_sequence 1 ggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtgaagaacacgtcctccgctccggtagacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtggagctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggcaccgcgcagttcggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgctcaacggcacgccctgcacggtcggcggcagc BBa_B0034_sequence 1 aaagaggagaaa BBa_K1319106_sequence 1 tactagatggccggc BBa_K314111_sequence 1 gtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggacatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtga BBa_K1615062_sequence 1 acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagaggtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggacatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatggccggcatgaccacctttccgaccctggatccggaactggcagcagcactgaccatgctgccgaaagttgattttgccgatctgccgaatgcacgtgccacctataatgcactgattggtgcaatgctggcggatctgccgtttgatggtgttagcctgcgtgaactgagcgcaccgggtctggatggtgatccggaagttaccattcgtttttttacaccggataataccgcaggtccggttccggttctgctgtggattcatggtggtggttttgcaattggcaccgcagaaagcagcgatccgttttgtgttgaagttgcacgtgaactgggttttgcagttgcaaatgttgaatatcgtctggcaccggaaaccagctttccgggtccggttaatgattgttatgcagcactgctgtatattcatgcacatgccgaagaactgggtgttgatccgagccgtattgcagttggtggtcagagtgccggtggtggtctggcagccggtacagttctgaaagcacgtgatgaaggtgttgttccggttgcatttcagtttctggaaattcctgaactggatgatcgcctggcaaccgttagcatgaccaattttgttgatacaccgctgtggcatcgtccgaatgcaattctgagctggaaatattacctgggtgaaagctatagcggtccggaagatccggatgtgagcatttatgccgcaccgagccgtgcaaccgatctgacaggtctgcctccgacctatctgagcaccatggaactggatccgctgcgtgatgagggtctggaatatgcactgcgtctgctgcatgccggtgttagcgttgaactgcatagttttccgggtacatttcatggtagcgcactggttgccaccgcagcagttagcgaacgtggtgcagcagaagccctgacagccctgcgtcgtggtctgcgtagcctgagtccggttagcaccggcggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtgaagaacacgtcctccgctccggtagacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtggagctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggcaccgcgcagttcggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgctcaacggcacgccctgcacggtcggcggcagc BBa_K1615045_sequence 1 atgaccacctttccgaccctggatccggaactggcagcagcactgaccatgctgccgaaagttgattttgccgatctgccgaatgcacgtgccacctataatgcactgattggtgcaatgctggcggatctgccgtttgatggtgttagcctgcgtgaactgagcgcaccgggtctggatggtgatccggaagttaccattcgtttttttacaccggataataccgcaggtccggttccggttctgctgtggattcatggtggtggttttgcaattggcaccgcagaaagcagcgatccgttttgtgttgaagttgcacgtgaactgggttttgcagttgcaaatgttgaatatcgtctggcaccggaaaccagctttccgggtccggttaatgattgttatgcagcactgctgtatattcatgcacatgccgaagaactgggtgttgatccgagccgtattgcagttggtggtcagagtgccggtggtggtctggcagccggtacagttctgaaagcacgtgatgaaggtgttgttccggttgcatttcagtttctggaaattcctgaactggatgatcgcctggcaaccgttagcatgaccaattttgttgatacaccgctgtggcatcgtccgaatgcaattctgagctggaaatattacctgggtgaaagctatagcggtccggaagatccggatgtgagcatttatgccgcaccgagccgtgcaaccgatctgacaggtctgcctccgacctatctgagcaccatggaactggatccgctgcgtgatgagggtctggaatatgcactgcgtctgctgcatgccggtgttagcgttgaactgcatagttttccgggtacatttcatggtagcgcactggttgccaccgcagcagttagcgaacgtggtgcagcagaagccctgacagccctgcgtcgtggtctgcgtagcctgagtccggttagc BBa_R0011_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca BBa_K1615052_sequence 1 atgaccacctttccgaccctggatccggaactggcagcagcactgaccatgctgccgaaagttgattttgccgatctgccgaatgcacgtgccacctataatgcactgattggtgcaatgctggcggatctgccgtttgatggtgttagcctgcgtgaactgagcgcaccgggtctggatggtgatccggaagttaccattcgtttttttacaccggataataccgcaggtccggttccggttctgctgtggattcatggtggtggttttgcaattggcaccgcagaaagcagcgatccgttttgtgttgaagttgcacgtgaactgggttttgcagttgcaaatgttgaatatcgtctggcaccggaaaccagctttccgggtccggttaatgattgttatgcagcactgctgtatattcatgcacatgccgaagaactgggtgttgatccgagccgtattgcagttggtggtcagagtgccggtggtggtctggcagccggtacagttctgaaagcacgtgatgaaggtgttgttccggttgcatttcagtttctggaaattcctgaactggatgatcgcctggcaaccgttagcatgaccaattttgttgatacaccgctgtggcatcgtccgaatgcaattctgagctggaaatattacctgggtgaaagctatagcggtccggaagatccggatgtgagcatttatgccgcaccgagccgtgcaaccgatctgacaggtctgcctccgacctatctgagcaccatggaactggatccgctgcgtgatgagggtctggaatatgcactgcgtctgctgcatgccggtgttagcgttgaactgcatagttttccgggtacatttcatggtagcgcactggttgccaccgcagcagttagcgaacgtggtgcagcagaagccctgacagccctgcgtcgtggtctgcgtagcctgagtccggttagcaccggcggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtgaagaacacgtcctccgctccggtagacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtggagctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggcaccgcgcagttcggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgctcaacggcacgccctgcacggtcggcggcagc BBa_K314103_sequence 1 acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagaggtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggacatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z