LOCUS       BBa_K1615065            3339 bp    DNA     linear       07-May-2024
DEFINITION  Linker-CBDcipA-linker fused to Heroin Esterase driven by LacI 
            promoter
ACCESSION   BBa_K1615065
VERSION     BBa_K1615065.1  
FEATURES             Location/Qualifiers
     misc_feature    18..324
                     /label=f1 origin
     misc_feature    1..455
                     /label=BBa_K314110
     misc_feature    464..1555
                     /label=BBa_K314111
     misc_feature    1566..1582
                     /label=lac O1
     promoter        1583..1588
                     /label=-35
     misc_feature    1589..1605
                     /label=lac O1
     promoter        1606..1611
                     /label=-10
     misc_feature    1564..1617
                     /label=BBa_R0011
     promoter        1564..1617
                     /label=BBa_R0011
     misc_feature    1631..1634
                     /label=conserved
     RBS             1627..1638
                     /label=BBa_B0034
     misc_feature    1..1638
                     /label=BBa_K314103
     misc_feature    1645..1647
                     /label=start
     misc_feature    1639..1653
                     /label=BBa_K1319106
     CDS             1654..2364
                     /label=BBa_K1615111
     misc_feature    2365..2370
                     /label=Scar 25
     misc_feature    2365..2370
                     /label=BBa_B0105
     CDS             2371..3339
                     /label=BBa_K1615045
     misc_feature    1654..3339
                     /label=BBa_K1615055
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgttta caatttacta gaggtggtga atgtgaaacc
      481 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
      541 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
      601 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
      661 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
      721 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
      781 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
      841 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
      901 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
      961 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
     1021 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
     1081 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
     1141 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
     1201 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
     1261 cgttggtgcg gacatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
     1321 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
     1381 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
     1441 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     1501 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgatacta
     1561 gagaattgtg agcggataac aattgacatt gtgagcggat aacaagatac tgagcacata
     1621 ctagagaaag aggagaaata ctagatggcc ggcaatgcaa ccccgaccaa aggtgccacc
     1681 ccgacaaata ccgccacacc gaccaaaagc gcaaccgcga ccccgacccg tccgagcgtt
     1741 ccgaccaata cccctaccaa tacaccggca aatactccgg ttagcggtaa tctgaaagtg
     1801 gaattttaca atagcaatcc gagcgatacc accaatagca ttaatccgca gtttaaagtt
     1861 accaataccg gcagcagcgc aattgatctg agcaaactga ccctgcgtta ttactatacc
     1921 gttgatggtc agaaagatca gacattttgg tgtgatcatg cagccattat tggtagcaat
     1981 ggtagctata atggcattac cagcaatgtg aaaggcacct tcgttaaaat gagcagcagc
     2041 acaaataatg ccgataccta tctggaaatt agctttacag gtggcaccct ggaaccgggt
     2101 gcacatgttc agattcaggg tcgttttgcc aaaaatgatt ggagcaatta tacccagagc
     2161 aacgactata gctttaaaag cgccagccag tttgttgaat gggatcaggt taccgcatat
     2221 ctgaatggtg ttctggtttg gggtaaagaa cctggtggta gcgttgttcc gagcacccag
     2281 ccggttacca cccctccggc aaccaccaaa ccgcctgcca ccacaaaacc accggcaaca
     2341 accattccgc ctagtgatga tccgaccggc atgaccacct ttccgaccct ggatccggaa
     2401 ctggcagcag cactgaccat gctgccgaaa gttgattttg ccgatctgcc gaatgcacgt
     2461 gccacctata atgcactgat tggtgcaatg ctggcggatc tgccgtttga tggtgttagc
     2521 ctgcgtgaac tgagcgcacc gggtctggat ggtgatccgg aagttaccat tcgttttttt
     2581 acaccggata ataccgcagg tccggttccg gttctgctgt ggattcatgg tggtggtttt
     2641 gcaattggca ccgcagaaag cagcgatccg ttttgtgttg aagttgcacg tgaactgggt
     2701 tttgcagttg caaatgttga atatcgtctg gcaccggaaa ccagctttcc gggtccggtt
     2761 aatgattgtt atgcagcact gctgtatatt catgcacatg ccgaagaact gggtgttgat
     2821 ccgagccgta ttgcagttgg tggtcagagt gccggtggtg gtctggcagc cggtacagtt
     2881 ctgaaagcac gtgatgaagg tgttgttccg gttgcatttc agtttctgga aattcctgaa
     2941 ctggatgatc gcctggcaac cgttagcatg accaattttg ttgatacacc gctgtggcat
     3001 cgtccgaatg caattctgag ctggaaatat tacctgggtg aaagctatag cggtccggaa
     3061 gatccggatg tgagcattta tgccgcaccg agccgtgcaa ccgatctgac aggtctgcct
     3121 ccgacctatc tgagcaccat ggaactggat ccgctgcgtg atgagggtct ggaatatgca
     3181 ctgcgtctgc tgcatgccgg tgttagcgtt gaactgcata gttttccggg tacatttcat
     3241 ggtagcgcac tggttgccac cgcagcagtt agcgaacgtg gtgcagcaga agccctgaca
     3301 gccctgcgtc gtggtctgcg tagcctgagt ccggttagc
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