LOCUS       BBa_K1615066            3339 bp    DNA     linear       22-Dec-2024
DEFINITION  Heroin Esterase fused to Linker-CBDcipA-linker driven by LacI 
            promoter
ACCESSION   BBa_K1615066
VERSION     BBa_K1615066.1  
FEATURES             Location/Qualifiers
     misc_feature    18..324
                     /label=f1 origin
     misc_feature    1..455
                     /label=BBa_K314110
     misc_feature    464..1555
                     /label=BBa_K314111
     misc_feature    1566..1582
                     /label=lac O1
     promoter        1583..1588
                     /label=-35
     misc_feature    1589..1605
                     /label=lac O1
     promoter        1606..1611
                     /label=-10
     misc_feature    1564..1617
                     /label=BBa_R0011
     promoter        1564..1617
                     /label=BBa_R0011
     misc_feature    1631..1634
                     /label=conserved
     RBS             1627..1638
                     /label=BBa_B0034
     misc_feature    1..1638
                     /label=BBa_K314103
     misc_feature    1645..1647
                     /label=start
     misc_feature    1639..1653
                     /label=BBa_K1319106
     CDS             1654..2622
                     /label=BBa_K1615045
     misc_feature    2623..2628
                     /label=Scar 25
     misc_feature    2623..2628
                     /label=BBa_B0105
     CDS             2629..3339
                     /label=BBa_K1615111
     misc_feature    1654..3339
                     /label=BBa_K1615056
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgttta caatttacta gaggtggtga atgtgaaacc
      481 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
      541 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
      601 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
      661 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
      721 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
      781 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
      841 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
      901 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
      961 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
     1021 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
     1081 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
     1141 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
     1201 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
     1261 cgttggtgcg gacatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
     1321 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
     1381 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
     1441 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     1501 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgatacta
     1561 gagaattgtg agcggataac aattgacatt gtgagcggat aacaagatac tgagcacata
     1621 ctagagaaag aggagaaata ctagatggcc ggcatgacca cctttccgac cctggatccg
     1681 gaactggcag cagcactgac catgctgccg aaagttgatt ttgccgatct gccgaatgca
     1741 cgtgccacct ataatgcact gattggtgca atgctggcgg atctgccgtt tgatggtgtt
     1801 agcctgcgtg aactgagcgc accgggtctg gatggtgatc cggaagttac cattcgtttt
     1861 tttacaccgg ataataccgc aggtccggtt ccggttctgc tgtggattca tggtggtggt
     1921 tttgcaattg gcaccgcaga aagcagcgat ccgttttgtg ttgaagttgc acgtgaactg
     1981 ggttttgcag ttgcaaatgt tgaatatcgt ctggcaccgg aaaccagctt tccgggtccg
     2041 gttaatgatt gttatgcagc actgctgtat attcatgcac atgccgaaga actgggtgtt
     2101 gatccgagcc gtattgcagt tggtggtcag agtgccggtg gtggtctggc agccggtaca
     2161 gttctgaaag cacgtgatga aggtgttgtt ccggttgcat ttcagtttct ggaaattcct
     2221 gaactggatg atcgcctggc aaccgttagc atgaccaatt ttgttgatac accgctgtgg
     2281 catcgtccga atgcaattct gagctggaaa tattacctgg gtgaaagcta tagcggtccg
     2341 gaagatccgg atgtgagcat ttatgccgca ccgagccgtg caaccgatct gacaggtctg
     2401 cctccgacct atctgagcac catggaactg gatccgctgc gtgatgaggg tctggaatat
     2461 gcactgcgtc tgctgcatgc cggtgttagc gttgaactgc atagttttcc gggtacattt
     2521 catggtagcg cactggttgc caccgcagca gttagcgaac gtggtgcagc agaagccctg
     2581 acagccctgc gtcgtggtct gcgtagcctg agtccggtta gcaccggcaa tgcaaccccg
     2641 accaaaggtg ccaccccgac aaataccgcc acaccgacca aaagcgcaac cgcgaccccg
     2701 acccgtccga gcgttccgac caatacccct accaatacac cggcaaatac tccggttagc
     2761 ggtaatctga aagtggaatt ttacaatagc aatccgagcg ataccaccaa tagcattaat
     2821 ccgcagttta aagttaccaa taccggcagc agcgcaattg atctgagcaa actgaccctg
     2881 cgttattact ataccgttga tggtcagaaa gatcagacat tttggtgtga tcatgcagcc
     2941 attattggta gcaatggtag ctataatggc attaccagca atgtgaaagg caccttcgtt
     3001 aaaatgagca gcagcacaaa taatgccgat acctatctgg aaattagctt tacaggtggc
     3061 accctggaac cgggtgcaca tgttcagatt cagggtcgtt ttgccaaaaa tgattggagc
     3121 aattataccc agagcaacga ctatagcttt aaaagcgcca gccagtttgt tgaatgggat
     3181 caggttaccg catatctgaa tggtgttctg gtttggggta aagaacctgg tggtagcgtt
     3241 gttccgagca cccagccggt taccacccct ccggcaacca ccaaaccgcc tgccaccaca
     3301 aaaccaccgg caacaaccat tccgcctagt gatgatccg
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