LOCUS BBa_K1615079 3627 bp DNA linear 26-Nov-2024 DEFINITION CBDcenA+Linker fused to TVEL5 driven by LacI promoter ACCESSION BBa_K1615079 VERSION BBa_K1615079.1 FEATURES Location/Qualifiers misc_feature 18..324 /label=f1 origin misc_feature 1..455 /label=BBa_K314110 misc_feature 464..1555 /label=BBa_K314111 misc_feature 1566..1582 /label=lac O1 promoter 1583..1588 /label=-35 misc_feature 1589..1605 /label=lac O1 promoter 1606..1611 /label=-10 misc_feature 1564..1617 /label=BBa_R0011 promoter 1564..1617 /label=BBa_R0011 misc_feature 1631..1634 /label=conserved RBS 1627..1638 /label=BBa_B0034 misc_feature 1..1638 /label=BBa_K314103 misc_feature 1645..1647 /label=start misc_feature 1639..1653 /label=BBa_K1319106 CDS 1972..2061 /label=Linker: Pro-Thr box CDS 1654..1971 /label=CBDCenA CDS 1654..2061 /label=BBa_K1321339 misc_feature 2062..2067 /label=Scar 25 misc_feature 2062..2067 /label=BBa_B0105 CDS 2068..3627 /label=BBa_K1615067 misc_feature 1654..3627 /label=BBa_K1615069 ORIGIN 1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 421 acgcgaattt taacaaaata ttaacgttta caatttacta gaggtggtga atgtgaaacc 481 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt 541 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc 601 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct 661 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat 721 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg 781 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat 841 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt 901 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca 961 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc 1021 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa 1081 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat 1141 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct 1201 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg 1261 cgttggtgcg gacatctcgg tagtgggata cgacgatacc gaagacagct catgttatat 1321 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg 1381 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact 1441 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 1501 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgatacta 1561 gagaattgtg agcggataac aattgacatt gtgagcggat aacaagatac tgagcacata 1621 ctagagaaag aggagaaata ctagatggcc ggcgcgccgg gttgccgtgt tgactacgcg 1681 gttaccaacc agtggccggg tggtttcggt gcgaacgtta ccatcaccaa cctgggtgac 1741 ccggtttctt cttggaaact ggactggacc tacaccgcgg gtcagcgtat ccagcagctg 1801 tggaacggta ccgcgtctac caacggtggt caggtttctg ttacctctct gccgtggaac 1861 ggttctatcc cgactggtgg taccgcgtct ttcggtttca acggttcttg ggcgggttct 1921 aacccgaccc cggcgtcttt ctctctgaac ggtaccacct gcacgggtac cccgacgacc 1981 tcgccaactc ctacgccgac cccaaccacg ccgactccta caccgacgcc caccccaacg 2041 cctactccga cggtcacccc caccggcatg tctcgtttcc atagcctttt agcattcgtg 2101 gtcgcgtccc tggccgcggt ggcgcacgca ggcatcgggc cagtagctga tctgacgatt 2161 acgaatgcag cagtgagtcc tgatggattc tcccgtcagg cagtcgtggt aaatggtggc 2221 actcccgggc ccctgatcac aggtaatatg ggggatcgtt ttcagctgaa cgtgattgat 2281 aatctgacta accatacgat gttaaaatcc accagcattc attggcatgg ctttttccag 2341 aaaggtacga attgggcaga tggaccggcg tttatcaatc agtgccctat tagcagcggg 2401 catagctttc tgtacgattt ccaggtgccg gatcaagcgg gcacgttctg gtaccattct 2461 cacctgagca cgcagtactg cgacggtctc cgtggacctt tcgttgtgta tgatccgaac 2521 gacccggccg ccgatttgta cgacgttgac aacgacgaca ccgtcattac cctcgcggac 2581 tggtaccacg tcgccgcaaa attgggcccg gcgtttcccc tgggcgccga tgcaacgctg 2641 atcaatggca aagggcgcag tccaagtacg acaacagcag atctcaccgt gatttctgtc 2701 accccgggta aacgttatcg cttccgcctg gtatccctta gctgcgatcc gaaccacacg 2761 ttttctattg acggtcacaa catgacaatt attgagacag atagcattaa caccgccccc 2821 ctggtggtcg actcaatcca gatctttgct gcgcagcgct actcatttgt tctggaagcc 2881 aaccaggcag ttgataatta ttggatccgt gcgaaccctt cgttcggcaa tgttgggttc 2941 actggcggca tcaactccgc catcctgcgc tacgacgggg cggcggcgat tgaaccgacc 3001 actacccaga ccacgtcgac tgaaccgctt aacgaggtca atttgcaccc gctggttgcg 3061 acggcggtac cgggctctcc cgccgctggc ggagtggatt tggcgatcaa tatggcgttt 3121 aactttaatg gtacgaattt cttcattaac ggcgcttctt ttactcctcc gaccgtgccg 3181 gttctgctcc agattatttc gggggcacag aacgctcaag acctgttacc gtcaggcagc 3241 gtctactcgc tgccatctaa cgcggacatt gaaattagct ttccagctac cgcagctgca 3301 ccgggagcac cgcacccctt tcatctccat gggcacgcgt ttgctgtagt acgctctgcg 3361 ggctccacgg tttataacta cgacaatccg atttttcgtg atgtggtgtc gacgggtacg 3421 cccgcagctg gcgacaacgt cacaattcgt ttccggacgg ataatcccgg tccttggttt 3481 ctccattgcc atatcgactt tcacctcgaa gcagggtttg ccgtagtctt tgccgaagat 3541 atccctgatg tcgcgtccgc gaacccggtg ccgcaagcgt ggtcggatct ttgtccgacc 3601 tatgacgccc gcgaccctag ctaccag //