LOCUS BBa_K1615081 3601 bp DNA linear 23-Dec-2024 DEFINITION Linker+dCBD fused to TVEL5 driven by LacI promoter ACCESSION BBa_K1615081 VERSION BBa_K1615081.1 FEATURES Location/Qualifiers misc_feature 18..324 /label=f1 origin misc_feature 1..455 /label=BBa_K314110 misc_feature 464..1555 /label=BBa_K314111 misc_feature 1566..1582 /label=lac O1 promoter 1583..1588 /label=-35 misc_feature 1589..1605 /label=lac O1 promoter 1606..1611 /label=-10 misc_feature 1564..1617 /label=BBa_R0011 promoter 1564..1617 /label=BBa_R0011 misc_feature 1631..1634 /label=conserved RBS 1627..1638 /label=BBa_B0034 misc_feature 1..1638 /label=BBa_K314103 misc_feature 1653..1655 /label=start misc_feature 1647..1661 /label=BBa_K1319106 CDS 1670..2035 /label=dCBD CDS 1670..1741 /label=Linker misc_feature 1742..1855 /label=cbh2 CBD CDS 1856..1927 /label=Linker misc_feature 1928..2035 /label=cbh1 CBD CDS 1670..2035 /label=BBa_K1321340 misc_feature 2036..2041 /label=Scar 25 misc_feature 2036..2041 /label=BBa_B0105 CDS 2042..3601 /label=BBa_K1615067 misc_feature 1670..3601 /label=BBa_K1615071 ORIGIN 1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 421 acgcgaattt taacaaaata ttaacgttta caatttacta gaggtggtga atgtgaaacc 481 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt 541 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc 601 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct 661 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat 721 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg 781 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat 841 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt 901 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca 961 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc 1021 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa 1081 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat 1141 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct 1201 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg 1261 cgttggtgcg gacatctcgg tagtgggata cgacgatacc gaagacagct catgttatat 1321 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg 1381 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact 1441 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 1501 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgatacta 1561 gagaattgtg agcggataac aattgacatt gtgagcggat aacaagatac tgagcacata 1621 ctagagaaag aggagaaata ctagagtact agatggccgg ctactagagc ccggcgccaa 1681 tccgcccggt actacgacca cttcgcgtcc agcaaccacg accgggtcga gccccggccc 1741 acaggcctgt tcttccgtct ggggtcagtg cggcggacag aactggtctg gccctacctg 1801 ctgtgcttct ggcagcacat gcgtctactc taacgactac tatagccagt gcctgccggg 1861 tgctaacccg ccaggcacta ccactacctc tcgccccgct accactacag gctctagccc 1921 gggacctacc caaagccact acggccagtg cggaggcatt ggctacagcg gccctaccgt 1981 ctgcgcctcc ggaaccacct gccaagtcct gaacccgtac tactcccagt gtcttaccgg 2041 catgtctcgt ttccatagcc ttttagcatt cgtggtcgcg tccctggccg cggtggcgca 2101 cgcaggcatc gggccagtag ctgatctgac gattacgaat gcagcagtga gtcctgatgg 2161 attctcccgt caggcagtcg tggtaaatgg tggcactccc gggcccctga tcacaggtaa 2221 tatgggggat cgttttcagc tgaacgtgat tgataatctg actaaccata cgatgttaaa 2281 atccaccagc attcattggc atggcttttt ccagaaaggt acgaattggg cagatggacc 2341 ggcgtttatc aatcagtgcc ctattagcag cgggcatagc tttctgtacg atttccaggt 2401 gccggatcaa gcgggcacgt tctggtacca ttctcacctg agcacgcagt actgcgacgg 2461 tctccgtgga cctttcgttg tgtatgatcc gaacgacccg gccgccgatt tgtacgacgt 2521 tgacaacgac gacaccgtca ttaccctcgc ggactggtac cacgtcgccg caaaattggg 2581 cccggcgttt cccctgggcg ccgatgcaac gctgatcaat ggcaaagggc gcagtccaag 2641 tacgacaaca gcagatctca ccgtgatttc tgtcaccccg ggtaaacgtt atcgcttccg 2701 cctggtatcc cttagctgcg atccgaacca cacgttttct attgacggtc acaacatgac 2761 aattattgag acagatagca ttaacaccgc ccccctggtg gtcgactcaa tccagatctt 2821 tgctgcgcag cgctactcat ttgttctgga agccaaccag gcagttgata attattggat 2881 ccgtgcgaac ccttcgttcg gcaatgttgg gttcactggc ggcatcaact ccgccatcct 2941 gcgctacgac ggggcggcgg cgattgaacc gaccactacc cagaccacgt cgactgaacc 3001 gcttaacgag gtcaatttgc acccgctggt tgcgacggcg gtaccgggct ctcccgccgc 3061 tggcggagtg gatttggcga tcaatatggc gtttaacttt aatggtacga atttcttcat 3121 taacggcgct tcttttactc ctccgaccgt gccggttctg ctccagatta tttcgggggc 3181 acagaacgct caagacctgt taccgtcagg cagcgtctac tcgctgccat ctaacgcgga 3241 cattgaaatt agctttccag ctaccgcagc tgcaccggga gcaccgcacc cctttcatct 3301 ccatgggcac gcgtttgctg tagtacgctc tgcgggctcc acggtttata actacgacaa 3361 tccgattttt cgtgatgtgg tgtcgacggg tacgcccgca gctggcgaca acgtcacaat 3421 tcgtttccgg acggataatc ccggtccttg gtttctccat tgccatatcg actttcacct 3481 cgaagcaggg tttgccgtag tctttgccga agatatccct gatgtcgcgt ccgcgaaccc 3541 ggtgccgcaa gcgtggtcgg atctttgtcc gacctatgac gcccgcgacc ctagctacca 3601 g //