BBa_K1615091 1 BBa_K1615091 CBDcenA+Linker fused to mRFP in RFC25 2015-08-30T11:00:00Z 2015-08-31T04:31:50Z mRFP highly engineered variant originally from Discosoma striata. CBDcenA from Cellulomonas fimi. mRFP fused C terminally to CBDcenA + C-terminal linker. false false _2032_ 25547 25547 9 false Fusion done using RFC25. false Brooke Rothschild-Mancinelli component2440590 1 BBa_K1615089 component2440586 1 BBa_K1321339 component2440588 1 BBa_B0105 annotation2440588 1 BBa_B0105 range2440588 1 409 414 annotation2440590 1 BBa_K1615089 range2440590 1 415 1089 annotation2440586 1 BBa_K1321339 range2440586 1 1 408 BBa_K1321339 1 BBa_K1321339 CBDcenA+Linker, RFC 25 standard 2014-10-07T11:00:00Z 2015-06-17T12:14:18Z asdf CBDcenA in Freiburg format (RFC 25). false false _1696_ 4206 20830 9 In stock true asdf false Chris N Micklem annotation2418065 1 CBDCenA range2418065 1 1 318 annotation2414614 1 Linker: Pro-Thr box range2414614 1 319 408 BBa_B0105 1 Scar 25 RFC 25 Scar Sequence 2009-10-14T11:00:00Z 2015-08-31T04:07:21Z BBF RFC 25 This is the scar produced by assembly using RFC 25. If you are assembling a composite part using RFC 25, you can insert this part and specify blunt assembly to get the desired sequence. false false _1_ 0 25 397 Not in stock false Simple DNA sequence false Randy Rettberg annotation2041621 1 Scar 25 range2041621 1 1 6 BBa_K1615089 1 mRFP mRFP RFC25 2015-08-30T11:00:00Z 2016-01-25T11:25:49Z mRFP highly engineered variant originally from Discosoma striata. Highly engineered monomeric mutant of RFP. Improved version of part BBa_E1010 to remove illegal AgeI sites and add RFC25 prefix and suffix. false false _2032_ 4206 25547 9 false Codon optimised for E. coli. RFC 25 compatible. false Brooke Rothschild-Mancinelli annotation2440569 1 mRFP range2440569 1 1 675 BBa_K1615089_sequence 1 atggcaagcagcgaagatgtgatcaaagaatttatgcgtttcaaagtgcgtatggaaggtagcgttaatggccatgaatttgaaattgaaggtgaaggcgaaggtcgtccgtatgaaggcacccagaccgcaaaactgaaagttaccaaaggtggtccgctgccgtttgcatgggatattctgagtccgcagtttcagtatggtagcaaagcatatgttaaacatccggcagatatcccggattatctgaaactgagctttccggaaggttttaaatgggaacgtgtgatgaattttgaagatggtggtgttgttaccgttacccaggatagcagcctgcaagatggtgaatttatctacaaagttaaactgcgtggcaccaactttccgagtgatggtccggttatgcagaaaaaaaccatgggttgggaagcaagcaccgaacgtatgtatccggaagatggcgcactgaaaggtgaaatcaaaatgcgcctgaaactgaaagatggtggccattatgatgcagaagttaaaaccacctacatggccaaaaaacctgttcagctgcctggtgcatataaaaccgatattaaactggatatcaccagccacaacgaggattataccattgttgaacagtatgaacgtgcagaaggtcgccatagcacaggtgca BBa_B0105_sequence 1 accggc BBa_K1321339_sequence 1 gcgccgggttgccgtgttgactacgcggttaccaaccagtggccgggtggtttcggtgcgaacgttaccatcaccaacctgggtgacccggtttcttcttggaaactggactggacctacaccgcgggtcagcgtatccagcagctgtggaacggtaccgcgtctaccaacggtggtcaggtttctgttacctctctgccgtggaacggttctatcccgactggtggtaccgcgtctttcggtttcaacggttcttgggcgggttctaacccgaccccggcgtctttctctctgaacggtaccacctgcacgggtaccccgacgacctcgccaactcctacgccgaccccaaccacgccgactcctacaccgacgcccaccccaacgcctactccgacggtcaccccc BBa_K1615091_sequence 1 gcgccgggttgccgtgttgactacgcggttaccaaccagtggccgggtggtttcggtgcgaacgttaccatcaccaacctgggtgacccggtttcttcttggaaactggactggacctacaccgcgggtcagcgtatccagcagctgtggaacggtaccgcgtctaccaacggtggtcaggtttctgttacctctctgccgtggaacggttctatcccgactggtggtaccgcgtctttcggtttcaacggttcttgggcgggttctaacccgaccccggcgtctttctctctgaacggtaccacctgcacgggtaccccgacgacctcgccaactcctacgccgaccccaaccacgccgactcctacaccgacgcccaccccaacgcctactccgacggtcacccccaccggcatggcaagcagcgaagatgtgatcaaagaatttatgcgtttcaaagtgcgtatggaaggtagcgttaatggccatgaatttgaaattgaaggtgaaggcgaaggtcgtccgtatgaaggcacccagaccgcaaaactgaaagttaccaaaggtggtccgctgccgtttgcatgggatattctgagtccgcagtttcagtatggtagcaaagcatatgttaaacatccggcagatatcccggattatctgaaactgagctttccggaaggttttaaatgggaacgtgtgatgaattttgaagatggtggtgttgttaccgttacccaggatagcagcctgcaagatggtgaatttatctacaaagttaaactgcgtggcaccaactttccgagtgatggtccggttatgcagaaaaaaaccatgggttgggaagcaagcaccgaacgtatgtatccggaagatggcgcactgaaaggtgaaatcaaaatgcgcctgaaactgaaagatggtggccattatgatgcagaagttaaaaccacctacatggccaaaaaacctgttcagctgcctggtgcatataaaaccgatattaaactggatatcaccagccacaacgaggattataccattgttgaacagtatgaacgtgcagaaggtcgccatagcacaggtgca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z