BBa_K1615089 1 mRFP mRFP RFC25 2015-08-30T11:00:00Z 2016-01-25T11:25:49Z mRFP highly engineered variant originally from Discosoma striata. Highly engineered monomeric mutant of RFP. Improved version of part BBa_E1010 to remove illegal AgeI sites and add RFC25 prefix and suffix. false false _2032_ 4206 25547 9 false Codon optimised for E. coli. RFC 25 compatible. false Brooke Rothschild-Mancinelli annotation2440569 1 mRFP range2440569 1 1 675 BBa_K1615100 1 BBa_K1615100 mRFP fused to Linker-CBDcipA-linker in RFC25 2015-08-30T11:00:00Z 2015-08-31T04:51:29Z mRFP highly engineered variant originally from Discosoma striata. CBDcipA from Clostridium thermocellum. mRFP fused N terminally to CBDcipA with N and C terminal linkers. false false _2032_ 25547 25547 9 false Fusion done using RFC25. false Brooke Rothschild-Mancinelli component2440644 1 BBa_K1615089 component2440647 1 BBa_K1615111 component2440646 1 BBa_B0105 annotation2440646 1 BBa_B0105 range2440646 1 676 681 annotation2440647 1 BBa_K1615111 range2440647 1 682 1392 annotation2440644 1 BBa_K1615089 range2440644 1 1 675 BBa_B0105 1 Scar 25 RFC 25 Scar Sequence 2009-10-14T11:00:00Z 2015-08-31T04:07:21Z BBF RFC 25 This is the scar produced by assembly using RFC 25. If you are assembling a composite part using RFC 25, you can insert this part and specify blunt assembly to get the desired sequence. false false _1_ 0 25 397 Not in stock false Simple DNA sequence false Randy Rettberg annotation2041621 1 Scar 25 range2041621 1 1 6 BBa_K1615111 1 CBDcipA CBDcipA with N and C-terminal linker in RFC25 2015-08-26T11:00:00Z 2015-08-27T04:18:48Z CBDcipA from Clostridium thermocellum CBDcipA with endogenous N and C terminal linkers from Clostridium thermocellum. This part is an improved version of BBa_K1321014, eliminating the illegal EcoRI site. This part is now BioBrick compatible using RFC25. false false _2032_ 25547 25547 9 false RFC25 Compatible. E. coli optimised. false Brooke Rothschild-Mancinelli BBa_K1615089_sequence 1 atggcaagcagcgaagatgtgatcaaagaatttatgcgtttcaaagtgcgtatggaaggtagcgttaatggccatgaatttgaaattgaaggtgaaggcgaaggtcgtccgtatgaaggcacccagaccgcaaaactgaaagttaccaaaggtggtccgctgccgtttgcatgggatattctgagtccgcagtttcagtatggtagcaaagcatatgttaaacatccggcagatatcccggattatctgaaactgagctttccggaaggttttaaatgggaacgtgtgatgaattttgaagatggtggtgttgttaccgttacccaggatagcagcctgcaagatggtgaatttatctacaaagttaaactgcgtggcaccaactttccgagtgatggtccggttatgcagaaaaaaaccatgggttgggaagcaagcaccgaacgtatgtatccggaagatggcgcactgaaaggtgaaatcaaaatgcgcctgaaactgaaagatggtggccattatgatgcagaagttaaaaccacctacatggccaaaaaacctgttcagctgcctggtgcatataaaaccgatattaaactggatatcaccagccacaacgaggattataccattgttgaacagtatgaacgtgcagaaggtcgccatagcacaggtgca BBa_B0105_sequence 1 accggc BBa_K1615111_sequence 1 aatgcaaccccgaccaaaggtgccaccccgacaaataccgccacaccgaccaaaagcgcaaccgcgaccccgacccgtccgagcgttccgaccaatacccctaccaatacaccggcaaatactccggttagcggtaatctgaaagtggaattttacaatagcaatccgagcgataccaccaatagcattaatccgcagtttaaagttaccaataccggcagcagcgcaattgatctgagcaaactgaccctgcgttattactataccgttgatggtcagaaagatcagacattttggtgtgatcatgcagccattattggtagcaatggtagctataatggcattaccagcaatgtgaaaggcaccttcgttaaaatgagcagcagcacaaataatgccgatacctatctggaaattagctttacaggtggcaccctggaaccgggtgcacatgttcagattcagggtcgttttgccaaaaatgattggagcaattatacccagagcaacgactatagctttaaaagcgccagccagtttgttgaatgggatcaggttaccgcatatctgaatggtgttctggtttggggtaaagaacctggtggtagcgttgttccgagcacccagccggttaccacccctccggcaaccaccaaaccgcctgccaccacaaaaccaccggcaacaaccattccgcctagtgatgatccg BBa_K1615100_sequence 1 atggcaagcagcgaagatgtgatcaaagaatttatgcgtttcaaagtgcgtatggaaggtagcgttaatggccatgaatttgaaattgaaggtgaaggcgaaggtcgtccgtatgaaggcacccagaccgcaaaactgaaagttaccaaaggtggtccgctgccgtttgcatgggatattctgagtccgcagtttcagtatggtagcaaagcatatgttaaacatccggcagatatcccggattatctgaaactgagctttccggaaggttttaaatgggaacgtgtgatgaattttgaagatggtggtgttgttaccgttacccaggatagcagcctgcaagatggtgaatttatctacaaagttaaactgcgtggcaccaactttccgagtgatggtccggttatgcagaaaaaaaccatgggttgggaagcaagcaccgaacgtatgtatccggaagatggcgcactgaaaggtgaaatcaaaatgcgcctgaaactgaaagatggtggccattatgatgcagaagttaaaaccacctacatggccaaaaaacctgttcagctgcctggtgcatataaaaccgatattaaactggatatcaccagccacaacgaggattataccattgttgaacagtatgaacgtgcagaaggtcgccatagcacaggtgcaaccggcaatgcaaccccgaccaaaggtgccaccccgacaaataccgccacaccgaccaaaagcgcaaccgcgaccccgacccgtccgagcgttccgaccaatacccctaccaatacaccggcaaatactccggttagcggtaatctgaaagtggaattttacaatagcaatccgagcgataccaccaatagcattaatccgcagtttaaagttaccaataccggcagcagcgcaattgatctgagcaaactgaccctgcgttattactataccgttgatggtcagaaagatcagacattttggtgtgatcatgcagccattattggtagcaatggtagctataatggcattaccagcaatgtgaaaggcaccttcgttaaaatgagcagcagcacaaataatgccgatacctatctggaaattagctttacaggtggcaccctggaaccgggtgcacatgttcagattcagggtcgttttgccaaaaatgattggagcaattatacccagagcaacgactatagctttaaaagcgccagccagtttgttgaatgggatcaggttaccgcatatctgaatggtgttctggtttggggtaaagaacctggtggtagcgttgttccgagcacccagccggttaccacccctccggcaaccaccaaaccgcctgccaccacaaaaccaccggcaacaaccattccgcctagtgatgatccg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z