LOCUS       BBa_K1615101            2742 bp    DNA     linear       22-Nov-2024
DEFINITION  CBDcenA+Linker fused to mRFP driven by LacI promoter
ACCESSION   BBa_K1615101
VERSION     BBa_K1615101.1  
FEATURES             Location/Qualifiers
     misc_feature    18..324
                     /label=f1 origin
     misc_feature    1..455
                     /label=BBa_K314110
     misc_feature    464..1555
                     /label=BBa_K314111
     misc_feature    1566..1582
                     /label=lac O1
     promoter        1583..1588
                     /label=-35
     misc_feature    1589..1605
                     /label=lac O1
     promoter        1606..1611
                     /label=-10
     misc_feature    1564..1617
                     /label=BBa_R0011
     promoter        1564..1617
                     /label=BBa_R0011
     misc_feature    1631..1634
                     /label=conserved
     RBS             1627..1638
                     /label=BBa_B0034
     misc_feature    1..1638
                     /label=BBa_K314103
     misc_feature    1645..1647
                     /label=start
     misc_feature    1639..1653
                     /label=BBa_K1319106
     CDS             1972..2061
                     /label=Linker: Pro-Thr box
     CDS             1654..1971
                     /label=CBDCenA
     CDS             1654..2061
                     /label=BBa_K1321339
     misc_feature    2062..2067
                     /label=Scar 25
     misc_feature    2062..2067
                     /label=BBa_B0105
     CDS             2068..2742
                     /label=mRFP
     CDS             2068..2742
                     /label=BBa_K1615089
     misc_feature    1654..2742
                     /label=BBa_K1615091
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgttta caatttacta gaggtggtga atgtgaaacc
      481 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
      541 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
      601 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
      661 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
      721 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
      781 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
      841 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
      901 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
      961 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
     1021 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
     1081 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
     1141 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
     1201 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
     1261 cgttggtgcg gacatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
     1321 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
     1381 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
     1441 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     1501 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgatacta
     1561 gagaattgtg agcggataac aattgacatt gtgagcggat aacaagatac tgagcacata
     1621 ctagagaaag aggagaaata ctagatggcc ggcgcgccgg gttgccgtgt tgactacgcg
     1681 gttaccaacc agtggccggg tggtttcggt gcgaacgtta ccatcaccaa cctgggtgac
     1741 ccggtttctt cttggaaact ggactggacc tacaccgcgg gtcagcgtat ccagcagctg
     1801 tggaacggta ccgcgtctac caacggtggt caggtttctg ttacctctct gccgtggaac
     1861 ggttctatcc cgactggtgg taccgcgtct ttcggtttca acggttcttg ggcgggttct
     1921 aacccgaccc cggcgtcttt ctctctgaac ggtaccacct gcacgggtac cccgacgacc
     1981 tcgccaactc ctacgccgac cccaaccacg ccgactccta caccgacgcc caccccaacg
     2041 cctactccga cggtcacccc caccggcatg gcaagcagcg aagatgtgat caaagaattt
     2101 atgcgtttca aagtgcgtat ggaaggtagc gttaatggcc atgaatttga aattgaaggt
     2161 gaaggcgaag gtcgtccgta tgaaggcacc cagaccgcaa aactgaaagt taccaaaggt
     2221 ggtccgctgc cgtttgcatg ggatattctg agtccgcagt ttcagtatgg tagcaaagca
     2281 tatgttaaac atccggcaga tatcccggat tatctgaaac tgagctttcc ggaaggtttt
     2341 aaatgggaac gtgtgatgaa ttttgaagat ggtggtgttg ttaccgttac ccaggatagc
     2401 agcctgcaag atggtgaatt tatctacaaa gttaaactgc gtggcaccaa ctttccgagt
     2461 gatggtccgg ttatgcagaa aaaaaccatg ggttgggaag caagcaccga acgtatgtat
     2521 ccggaagatg gcgcactgaa aggtgaaatc aaaatgcgcc tgaaactgaa agatggtggc
     2581 cattatgatg cagaagttaa aaccacctac atggccaaaa aacctgttca gctgcctggt
     2641 gcatataaaa ccgatattaa actggatatc accagccaca acgaggatta taccattgtt
     2701 gaacagtatg aacgtgcaga aggtcgccat agcacaggtg ca
//