LOCUS       BBa_K1615102            2742 bp    DNA     linear       22-Dec-2024
DEFINITION  mRFP used to CBDcenA+Linker in RFC25 driven by LacI promoter
ACCESSION   BBa_K1615102
VERSION     BBa_K1615102.1  
FEATURES             Location/Qualifiers
     misc_feature    18..324
                     /label=f1 origin
     misc_feature    1..455
                     /label=BBa_K314110
     misc_feature    464..1555
                     /label=BBa_K314111
     misc_feature    1566..1582
                     /label=lac O1
     promoter        1583..1588
                     /label=-35
     misc_feature    1589..1605
                     /label=lac O1
     promoter        1606..1611
                     /label=-10
     misc_feature    1564..1617
                     /label=BBa_R0011
     promoter        1564..1617
                     /label=BBa_R0011
     misc_feature    1631..1634
                     /label=conserved
     RBS             1627..1638
                     /label=BBa_B0034
     misc_feature    1..1638
                     /label=BBa_K314103
     misc_feature    1645..1647
                     /label=start
     misc_feature    1639..1653
                     /label=BBa_K1319106
     CDS             1654..2328
                     /label=mRFP
     CDS             1654..2328
                     /label=BBa_K1615089
     misc_feature    2329..2334
                     /label=Scar 25
     misc_feature    2329..2334
                     /label=BBa_B0105
     CDS             2653..2742
                     /label=Linker: Pro-Thr box
     CDS             2335..2652
                     /label=CBDCenA
     CDS             2335..2742
                     /label=BBa_K1321339
     misc_feature    1654..2742
                     /label=BBa_K1615092
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgttta caatttacta gaggtggtga atgtgaaacc
      481 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
      541 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
      601 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
      661 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
      721 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
      781 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
      841 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
      901 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
      961 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
     1021 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
     1081 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
     1141 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
     1201 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
     1261 cgttggtgcg gacatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
     1321 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
     1381 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
     1441 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     1501 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgatacta
     1561 gagaattgtg agcggataac aattgacatt gtgagcggat aacaagatac tgagcacata
     1621 ctagagaaag aggagaaata ctagatggcc ggcatggcaa gcagcgaaga tgtgatcaaa
     1681 gaatttatgc gtttcaaagt gcgtatggaa ggtagcgtta atggccatga atttgaaatt
     1741 gaaggtgaag gcgaaggtcg tccgtatgaa ggcacccaga ccgcaaaact gaaagttacc
     1801 aaaggtggtc cgctgccgtt tgcatgggat attctgagtc cgcagtttca gtatggtagc
     1861 aaagcatatg ttaaacatcc ggcagatatc ccggattatc tgaaactgag ctttccggaa
     1921 ggttttaaat gggaacgtgt gatgaatttt gaagatggtg gtgttgttac cgttacccag
     1981 gatagcagcc tgcaagatgg tgaatttatc tacaaagtta aactgcgtgg caccaacttt
     2041 ccgagtgatg gtccggttat gcagaaaaaa accatgggtt gggaagcaag caccgaacgt
     2101 atgtatccgg aagatggcgc actgaaaggt gaaatcaaaa tgcgcctgaa actgaaagat
     2161 ggtggccatt atgatgcaga agttaaaacc acctacatgg ccaaaaaacc tgttcagctg
     2221 cctggtgcat ataaaaccga tattaaactg gatatcacca gccacaacga ggattatacc
     2281 attgttgaac agtatgaacg tgcagaaggt cgccatagca caggtgcaac cggcgcgccg
     2341 ggttgccgtg ttgactacgc ggttaccaac cagtggccgg gtggtttcgg tgcgaacgtt
     2401 accatcacca acctgggtga cccggtttct tcttggaaac tggactggac ctacaccgcg
     2461 ggtcagcgta tccagcagct gtggaacggt accgcgtcta ccaacggtgg tcaggtttct
     2521 gttacctctc tgccgtggaa cggttctatc ccgactggtg gtaccgcgtc tttcggtttc
     2581 aacggttctt gggcgggttc taacccgacc ccggcgtctt tctctctgaa cggtaccacc
     2641 tgcacgggta ccccgacgac ctcgccaact cctacgccga ccccaaccac gccgactcct
     2701 acaccgacgc ccaccccaac gcctactccg acggtcaccc cc
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