LOCUS       BBa_K1615105            2661 bp    DNA     linear       25-Nov-2024
DEFINITION  CBDcex fused to mRFP driven by LacI promoter
ACCESSION   BBa_K1615105
VERSION     BBa_K1615105.1  
FEATURES             Location/Qualifiers
     misc_feature    18..324
                     /label=f1 origin
     misc_feature    1..455
                     /label=BBa_K314110
     misc_feature    464..1555
                     /label=BBa_K314111
     misc_feature    1566..1582
                     /label=lac O1
     promoter        1583..1588
                     /label=-35
     misc_feature    1589..1605
                     /label=lac O1
     promoter        1606..1611
                     /label=-10
     misc_feature    1564..1617
                     /label=BBa_R0011
     promoter        1564..1617
                     /label=BBa_R0011
     misc_feature    1631..1634
                     /label=conserved
     RBS             1627..1638
                     /label=BBa_B0034
     misc_feature    1..1638
                     /label=BBa_K314103
     misc_feature    1645..1647
                     /label=start
     misc_feature    1639..1653
                     /label=BBa_K1319106
     CDS             1654..1980
                     /label=BBa_K1321003
     misc_feature    1981..1986
                     /label=Scar 25
     misc_feature    1981..1986
                     /label=BBa_B0105
     CDS             1987..2661
                     /label=mRFP
     CDS             1987..2661
                     /label=BBa_K1615089
     misc_feature    1654..2661
                     /label=BBa_K1615095
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgttta caatttacta gaggtggtga atgtgaaacc
      481 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
      541 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
      601 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
      661 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
      721 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
      781 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
      841 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
      901 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
      961 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
     1021 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
     1081 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
     1141 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
     1201 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
     1261 cgttggtgcg gacatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
     1321 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
     1381 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
     1441 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     1501 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgatacta
     1561 gagaattgtg agcggataac aattgacatt gtgagcggat aacaagatac tgagcacata
     1621 ctagagaaag aggagaaata ctagatggcc ggcggtccgg ccgggtgcca ggtgctgtgg
     1681 ggcgtcaacc agtggaacac cggcttcacc gcgaacgtca ccgtgaagaa cacgtcctcc
     1741 gctccggtag acggctggac gctcacgttc agcttcccgt ccggccagca ggtcacccag
     1801 gcgtggagct cgacggtcac gcagtccggc tcggccgtga cggtccgcaa cgccccgtgg
     1861 aacggctcga tcccggcggg cggcaccgcg cagttcggct tcaacggctc gcacacgggc
     1921 accaacgccg cgccgacggc gttctcgctc aacggcacgc cctgcacggt cggcggcagc
     1981 accggcatgg caagcagcga agatgtgatc aaagaattta tgcgtttcaa agtgcgtatg
     2041 gaaggtagcg ttaatggcca tgaatttgaa attgaaggtg aaggcgaagg tcgtccgtat
     2101 gaaggcaccc agaccgcaaa actgaaagtt accaaaggtg gtccgctgcc gtttgcatgg
     2161 gatattctga gtccgcagtt tcagtatggt agcaaagcat atgttaaaca tccggcagat
     2221 atcccggatt atctgaaact gagctttccg gaaggtttta aatgggaacg tgtgatgaat
     2281 tttgaagatg gtggtgttgt taccgttacc caggatagca gcctgcaaga tggtgaattt
     2341 atctacaaag ttaaactgcg tggcaccaac tttccgagtg atggtccggt tatgcagaaa
     2401 aaaaccatgg gttgggaagc aagcaccgaa cgtatgtatc cggaagatgg cgcactgaaa
     2461 ggtgaaatca aaatgcgcct gaaactgaaa gatggtggcc attatgatgc agaagttaaa
     2521 accacctaca tggccaaaaa acctgttcag ctgcctggtg catataaaac cgatattaaa
     2581 ctggatatca ccagccacaa cgaggattat accattgttg aacagtatga acgtgcagaa
     2641 ggtcgccata gcacaggtgc a
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