LOCUS BBa_K1615106 2661 bp DNA linear 22-Dec-2024 DEFINITION mRFP fused to CBDcex driven by LacI promoter ACCESSION BBa_K1615106 VERSION BBa_K1615106.1 FEATURES Location/Qualifiers misc_feature 18..324 /label=f1 origin misc_feature 1..455 /label=BBa_K314110 misc_feature 464..1555 /label=BBa_K314111 misc_feature 1566..1582 /label=lac O1 promoter 1583..1588 /label=-35 misc_feature 1589..1605 /label=lac O1 promoter 1606..1611 /label=-10 misc_feature 1564..1617 /label=BBa_R0011 promoter 1564..1617 /label=BBa_R0011 misc_feature 1631..1634 /label=conserved RBS 1627..1638 /label=BBa_B0034 misc_feature 1..1638 /label=BBa_K314103 misc_feature 1645..1647 /label=start misc_feature 1639..1653 /label=BBa_K1319106 CDS 1654..2328 /label=mRFP CDS 1654..2328 /label=BBa_K1615089 misc_feature 2329..2334 /label=Scar 25 misc_feature 2329..2334 /label=BBa_B0105 CDS 2335..2661 /label=BBa_K1321003 misc_feature 1654..2661 /label=BBa_K1615096 ORIGIN 1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 421 acgcgaattt taacaaaata ttaacgttta caatttacta gaggtggtga atgtgaaacc 481 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt 541 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc 601 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct 661 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat 721 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg 781 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat 841 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt 901 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca 961 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc 1021 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa 1081 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat 1141 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct 1201 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg 1261 cgttggtgcg gacatctcgg tagtgggata cgacgatacc gaagacagct catgttatat 1321 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg 1381 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact 1441 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 1501 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgatacta 1561 gagaattgtg agcggataac aattgacatt gtgagcggat aacaagatac tgagcacata 1621 ctagagaaag aggagaaata ctagatggcc ggcatggcaa gcagcgaaga tgtgatcaaa 1681 gaatttatgc gtttcaaagt gcgtatggaa ggtagcgtta atggccatga atttgaaatt 1741 gaaggtgaag gcgaaggtcg tccgtatgaa ggcacccaga ccgcaaaact gaaagttacc 1801 aaaggtggtc cgctgccgtt tgcatgggat attctgagtc cgcagtttca gtatggtagc 1861 aaagcatatg ttaaacatcc ggcagatatc ccggattatc tgaaactgag ctttccggaa 1921 ggttttaaat gggaacgtgt gatgaatttt gaagatggtg gtgttgttac cgttacccag 1981 gatagcagcc tgcaagatgg tgaatttatc tacaaagtta aactgcgtgg caccaacttt 2041 ccgagtgatg gtccggttat gcagaaaaaa accatgggtt gggaagcaag caccgaacgt 2101 atgtatccgg aagatggcgc actgaaaggt gaaatcaaaa tgcgcctgaa actgaaagat 2161 ggtggccatt atgatgcaga agttaaaacc acctacatgg ccaaaaaacc tgttcagctg 2221 cctggtgcat ataaaaccga tattaaactg gatatcacca gccacaacga ggattatacc 2281 attgttgaac agtatgaacg tgcagaaggt cgccatagca caggtgcaac cggcggtccg 2341 gccgggtgcc aggtgctgtg gggcgtcaac cagtggaaca ccggcttcac cgcgaacgtc 2401 accgtgaaga acacgtcctc cgctccggta gacggctgga cgctcacgtt cagcttcccg 2461 tccggccagc aggtcaccca ggcgtggagc tcgacggtca cgcagtccgg ctcggccgtg 2521 acggtccgca acgccccgtg gaacggctcg atcccggcgg gcggcaccgc gcagttcggc 2581 ttcaacggct cgcacacggg caccaacgcc gcgccgacgg cgttctcgct caacggcacg 2641 ccctgcacgg tcggcggcag c //