LOCUS       BBa_K1616002            1090 bp    DNA     linear       22-Dec-2024
DEFINITION  VVD linked to YN155 (YFP Nter split) with double terminator T7
ACCESSION   BBa_K1616002
VERSION     BBa_K1616002.1  
FEATURES             Location/Qualifiers
     RBS             1..12
                     /label=BBa_J61100
     CDS             21..470
                     /label=VVD
     CDS             21..470
                     /label=BBa_K1616014
     misc_feature    471..485
                     /label=linker JUN
     CDS             471..485
                     /label=BBa_K1616018
     CDS             486..953
                     /label=YN155
     CDS             486..953
                     /label=BBa_K1616017
     CDS             21..953
                     /label=BBa_K1616020
     stem_loop       973..1016
                     /label=stem_loop
     misc_feature    962..1041
                     /label=BBa_B0010
     terminator      962..1041
                     /label=BBa_B0010
     stem_loop       1057..1076
                     /label=T7 TE
     misc_feature    1083..1083
                     /label=stop
     polyA_site      1077..1090
                     /label=polya
     misc_feature    1050..1090
                     /label=BBa_B0012
     terminator      1050..1090
                     /label=BBa_B0012
     terminator      962..1090
                     /label=BBa_B0015
ORIGIN
        1 aaagagggga catactagag cacaccctgt atgcccctgg cggctacgac atcatgggct
       61 acctgatcca gatcatgaag cggcccaacc cccaggtgga actgggccct gtggatacct
      121 ctgtggccct gatcctgtgc gacctgaagc agaaagacac ccccatcgtg tacgcctccg
      181 aggccttcct gtacatgacc ggctactcca acgccgaggt gctgggccgg aactgtagat
      241 tcctccagtc ccctgacggc atggtcaagc ctaagtccac ccggaaatac gtggactcta
      301 acaccatcaa caccatgcgg aaggccatcg accggaacgc tgaggtgcag gtggaagtcg
      361 tgaacttcaa gaagaacggc cagcgcttcg tgaatttcct gaccatgatc cccgtgcggg
      421 acgagacagg cgagtacaga tactccatgg gcttccagtg cgagacagag agatccatcg
      481 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
      541 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
      601 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
      661 ccaccctcgt gaccaccttc ggctacggcc ttcaatgctt cgcccgctac cccgaccaca
      721 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
      781 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
      841 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
      901 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc tagtactaga
      961 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1021 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     1081 tgcgtttata
//