BBa_K1616003
1
BBa_K1616003
VVD link to YC155 (YFP Cter split)
2015-09-16T11:00:00Z
2015-09-18T03:39:05Z
Assembly the sequence of photoreceptor VVD (without illegal site), a linker(1) and then the C terminal of YFP split(1)
(1) Tom Kerppola, Ph. D, investigator at the Howard Hughes Medical Institute as well as Professor in the University of Michigan
Vivid (VVD) is the smallest known Light???oxygen???voltage (LOV) domain protein and photo-inducible dimer. Isolated from Neurospora crassa, VVD forms a homodimer in response to a blue-light stimulus.
Then, a split protein is a protein whose sequence has been divided into two (or more) different parts. The yellow-fluorescent (YFP) protein will only express fluorescence when its two parts will be reunited.
The part is coding for the homodimer VVD link by an integration of specific sequence to the C terminal of the YFP split.
true
false
_2033_
22805
22805
9
false
The sequence of VVD had 2 illegal sites PstI; that have been removed.
false
Johanna Chesnel
annotation2465730
1
YC155
range2465730
1
511
756
annotation2465726
1
VVD
range2465726
1
1
450
annotation2465727
1
Linker
range2465727
1
451
502
BBa_K1616003_sequence
1
cacaccctgtatgcccctggcggctacgacatcatgggctacctgatccagatcatgaagcggcccaacccccaggtggaactgggccctgtggatacctctgtggccctgatcctgtgcgacctgaagcagaaagacacccccatcgtgtacgcctccgaggccttcctgtacatgaccggctactccaacgccgaggtgctgggccggaactgtagattcctccagtcccctgacggcatggtcaagcctaagtccacccggaaatacgtggactctaacaccatcaacaccatgcggaaggccatcgaccggaacgctgaggtgcaggtggaagtcgtgaacttcaagaagaacggccagcgcttcgtgaatttcctgaccatgatccccgtgcgggacgagacaggcgagtacagatactccatgggcttccagtgcgagacagagcgtccggcgtgcaaaatcccgaacgacctgaaacagaaagtcatgaaccacgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z