BBa_K1616004
1
BBa_K1616004
VVD linked to YN155 (YFP Nter split)
2015-09-16T11:00:00Z
2015-09-18T03:40:44Z
Assembly the sequence of photoreceptor VVD (without illegal site), a linker(1) and then the N terminal of YFP split(1)
Vivid (VVD) is the smallest known Light???oxygen???voltage (LOV) domain protein and photo-inducible dimer. Isolated from Neurospora crassa, VVD forms a homodimer in response to a blue-light stimulus. Then, a split protein is a protein whose sequence has been divided into two (or more) different parts. The yellow-fluorescent (YFP) protein will only express fluorescence when its two parts will be reunited. The part is coding for the homodimer VVD link by an integration of specific sequence to the N terminal of the YFP split.
true
false
_2033_
22805
22805
9
false
(1) Tom Kerppola, Ph. D, investigator at the Howard Hughes Medical Institute as well as Professor in the University of Michigan
Hu CD, Chinenov Y, Kerppola TK. Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation. Mol Cell. 2002;9(4):789???98.
false
Johanna Chesnel
BBa_K1616004_sequence
1
cacaccctgtatgcccctggcggctacgacatcatgggctacctgatccagatcatgaagcggcccaacccccaggtggaactgggccctgtggatacctctgtggccctgatcctgtgcgacctgaagcagaaagacacccccatcgtgtacgcctccgaggccttcctgtacatgaccggctactccaacgccgaggtgctgggccggaactgtagattcctccagtcccctgacggcatggtcaagcctaagtccacccggaaatacgtggactctaacaccatcaacaccatgcggaaggccatcgaccggaacgctgaggtgcaggtggaagtcgtgaacttcaagaagaacggccagcgcttcgtgaatttcctgaccatgatccccgtgcgggacgagacaggcgagtacagatactccatgggcttccagtgcgagacagagagatccatcgccaccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggccttcaatgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggcctag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z