BBa_K1616007 1 Holin-rev Holin reversed 2015-09-16T11:00:00Z 2015-09-17T11:02:45Z This sequence is the reverses sequence of the BBa_K112805 (Holin). Holin works with endolysin; they create a toxic system, after a period of late-gene expression, holin, an inner permeabilizing protein creates holes in the membrane in order to permeabilize it and then endolysin, a muralytic enzyme traverses the holes creating by holin and degraded the wall cell. This complex induce cell lysis of bacteria. (Young & Bl??si, 1995) This part is the reversed sequence of the holin (BBa_K112805); thus this part can be used with a reversed endolysin (BBa_K1616006), reversed promoter, reversed RBS and reversed terminator. false false _2033_ 22805 22805 9 false The illegal sites have been checked false Johanna Chesnel annotation2466349 1 Holin range2466349 1 1 676 BBa_K1616007_sequence 1 taattatttagcccttcctaatattctggccgcctgagcacatattgattcaaggcggtcattacttatatgatcatttctataccagtacatggttatcgttccagcatagatattatccaaattaaaatatggacaactgtacatgtagtttatttcgggagtaggctttttagttggtaaaaaagcaaattttgagttggaataataatgacgtccatttaaatgaactgtatattcatccatagttttatcaacaggatatcctccaagtgatttttcacttattgttgaaggtaattttccttcgtatgctataatatcaacaaaatagtttaagtttttagggcggaaagaatacaccgcactaaagtctgcctcagatgatatatgaactatctggagttgttccagggcgacagattcaaagcgtgcagttctttccttttcaataatttcactgtatgtttcatactttgattgcttatagtactcaaagaaactatctcccctataccaaacaatcgccattataaacaaaagaattacgacagctacccgggaagcaagaaccttcccggtagcgttatctttgaacaagcgatcgagaacaccaaatagaatatcagagggcgaaaatgatattctaggtgctgccatagaccctccttttaag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z