BBa_K1616019 1 pDawn pDawn system 2015-09-17T11:00:00Z 2015-09-18T06:53:36Z The pDawn & pDusk system, have been optimized and constructed in the way that the toxins sequences we introduce in our construction are in fact the reverse sequences. After reading many papers to select an appropriate light-sensing system to use for the kill switch, our team came across the pDawn and pDusk plasmids. Those plasmids employ bluelight photoreceptors to confer light-repressed or light-induced gene expression in with up to 460-fold induction upon illumination. Controlled by this system, we thought to add toxins in it to kill our bacteria whenever we want(Ohlendorf, Vidavski, Eldar, Moffat, & M??glich, 2012). They are not dependent on nonnative chromophores that are often supplied exogenously and do not need any introduction of cofactor-synthesis genes. Moreover, they are practical to use since current optogenetic tools require multiple plasmid components. Finally, they deal with many limitations in a one-plasmid system which is based on the YF1/FixJ system. false false _2033_ 22805 22805 9 false The original sequence of pDawn have some illegal sites that have been removed. false Johanna Chesnel annotation2470877 1 FixK2 promoter range2470877 1 1125 1372 annotation2470880 1 YF1 range2470880 1 1743 2876 annotation2470867 1 cI-LVA range2470867 1 356 1102 annotation2470848 1 lambda pR promoter range2470848 1 136 184 annotation2470882 1 YF1/FixJ bicistronic operon range2470882 1 2876 3493 BBa_K1616019_sequence 1 gacccatttgctgtccaccagtcatgctagccatatggctgccgcgcggcaccaggccgctgctgtgatgatgatgatgatggctgctgcccatggtatatctccttcttaaagttaaacaaaattatttcttgagcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttactctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagcgatctacactagcactatcagcgttattaagctactaaagcgtagttttcgtcgtttgcagcgccaaacgtctcttcaggccactgactagcgataactttccccacaacggaacaactctcattgcatgggatcattgggtactgtgggtttagtggttgtaaaaacacctgaccgctatccctgatcagtttcttgaaggtaaactcatcacccccaagtctggctatgcagaaatcacctggctcaacagcctgctcagggtcaacgagaattaacattccgtcaggaaagctcggcttggagcctgttggtgcggtcatggaattaccttcaacctcaagccagaatgcagaatcactggcttttttggttgtgcttacccatctctccgcatcacctttggtaaaggttctaagctcaggtgagaacatccctgcctgaacatgagaaaaaacagggtactcatactcacttctaagtgacggctgcatactaaccgcttcatacatctcgtagatttctctggcgattgaagggctaaattcttcaacgctaactttgagaatttttgcaagcaatgcggcgttataagcatttaatgcattgatgccattaaataaagcaccaacgcctgactgccccatccccatcttgtctgcgacagattcctgggataagccaagttcatttttctttttttcataaattgctttaaggcgacgtgcgtcctcaagctgctcttgtgttaatggtttcttttttgtgctcatctagtatttctcctcttttcttgactccgttgtgatgacgcattggtacgcggtatcgggaggttcgaaaatttcgagcgatatcttaaggggggtgccttacgtagaaccccgtaggtcatgcccgaggccggtcctggatggcgcggcggatacgcttgagcaggttttcgtcgagaagcggcttcaaaaccacgtcttttacgccggcctcggcggcccgggtcgagatgttttcgtccggatagccggtgatcaggatcacgggcgtagatctcgatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgagatcccggacaccatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattgagggtggtgaatgtggctagttttcaatcatttgggataccaggacagctggaagtcatcaaaaaagcacttgatcacgtgcgagtcggtgtggtaattacagatcccgcacttgaagataatcctattgtctacgtaaatcaaggctttgttcaaatgaccggctacgagaccgaggaaattttaggaaagaactgtcgcttcttacaggggaaacacacagatcctgctgaagtggacaacatcagaaccgctttacaaaataaagaaccggtcaccgttcagatccaaaactacaaaaaagacggaacgatgttctggaatgaattaaatattgatccaatggaaatagaggataaaacgtattttgtcggtattcagaatgatatcaccgagcaccagcagacccaggcgcgcctccaggaactgcaatccgagctcgtccacgtctccaggctgagcgccatgggcgaaatggcgtccgcgctcgcgcacgagctcaaccagccgctggcggcgatcagcaactacatgaagggctcgcggcggctgcttgccggcagcagtgatccgaacacaccgaaggtcgaaagcgccctggaccgcgccgccgagcaggcgctgcgcgccggccagatcatccggcgcctgcgcgacttcgttgcccgcggcgaatcggagaagcgggtcgagagtctctccaagctgatcgaggaggccggcgcgctcgggcttgccggcgcgcgcgagcagaacgtgcagctccgcttcagtctcgatccgggcgccgatctcgttctcgccgaccgggtgcagatccagcaggtcctggtcaacctgttccgcaacgcgctggaagcgatggctcagtcgcagcgacgcgagctcgtcgtcaccaacacccccgccgccgacgacatgatcgaggtcgaagtgtccgacaccggcagcggtttccaggacgacgtcattccgaacctgtttcagactttcttcaccaccaaggacaccggcatgggcgtgggactgtccatcagccgctcgatcatcgaagctcacggcgggcgcatgtgggccgagagcaacgcatcgggcggggcgaccttccgcttcaccctcccggcagccgacgagatgataggaggtctagcatgacgaccaagggacatatctacgtcatcgacgacgacgcggcgatgcgggattcgctgaatttcctgctggattctgccggcttcggcgtcacgctgtttgacgacgcgcaagcctttctcgacgccctgccgggtctctccttcggctgcgtcgtctccgacgtgcgcatgccgggccttgacggcatcgagctgttgaagcggatgaaggcgcagcaaagcccctttccgatcctcatcatgaccggtcacggcgacgtgccgctcgcggtcgaggcgatgaagttaggggcggtggactttctggaaaagcctttcgaggacgaccgcctcaccgccatgatcgaatcggcgatccgccaggccgagccggccgccaagagcgaggccgtcgcgcaggatatcgccgcccgcgtcgcctcgttgagccccagggagcgccaggtcatggaagggctgatcgccggcctttccaacaagctgatcgcccgcgagtacgacatcagcccgcgcaccatcgaggtgtatcgggccaacgtcatgaccaagatgcaggccaacagcctttcggagctggttcgcctcgcgatgcgcgccggcatgctcaacgattga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z