LOCUS BBa_K1616022 4883 bp DNA linear 29-Nov-2024 DEFINITION pDawn - Endolysin/Holin ACCESSION BBa_K1616022 VERSION BBa_K1616022.1 FEATURES Location/Qualifiers misc_feature 1..41 /label=B0012 misc_feature 50..129 /label=B0010 terminator 1..129 /label=BBa_B0025 CDS 138..651 /label=Endolysin CDS 138..651 /label=BBa_K1616006 RBS 660..673 /label=RBS Reversed RBS 660..673 /label=BBa_K1616011 CDS 682..1357 /label=Holin CDS 682..1357 /label=BBa_K1616007 RBS 1366..1379 /label=RBS Reversed RBS 1366..1379 /label=BBa_K1616011 CDS 1..1379 /label=BBa_K1616005 promoter 1515..1563 /label=lambda pR promoter CDS 1735..2481 /label=cI-LVA promoter 2504..2751 /label=FixK2 promoter CDS 3122..4255 /label=YF1 misc_feature 4255..4872 /label=YF1/FixJ bicistronic operon promoter 1380..4883 /label=BBa_K1616019 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggt actagaggct ttatagattt ttatacgcgt cccaagtgcc agttctaaac 181 gttgtaatga ctcgttttgc gcgattaggt gtttgattat accatctact tttagctaag 241 ttaactgctg cttcatccca gcgtttttgt tgaagcatac gtaaagagtt agtaaatcct 301 gccacaccgg tttctcccat ttggaaaacc atattaatca atgcacagcg acgaaccgca 361 tcaagagaat cataaaccgg ttttaattta gcatttctca ggattccgcg aacagcagca 421 tcaacatcct gattaaagag tttttcagcc tcatcttttg taattacacc attgcaatta 481 cgcccaatag ctttatctaa ttcagattta gcagcattaa gtgatggact ttttgtaagc 541 aaatgaccga tgccaatagt gtaatagcct tctgtgtctt tatagatttt aagtctaaga 601 ccttcatcta tacgtaacat ttcaaatata ttcataatac ctcctaagta ttactagagt 661 tgtcccctct ttctactaga gtaattattt agcccttcct aatattctgg ccgcctgagc 721 acatattgat tcaaggcggt cattacttat atgatcattt ctataccagt acatggttat 781 cgttccagca tagatattat ccaaattaaa atatggacaa ctgtacatgt agtttatttc 841 gggagtaggc tttttagttg gtaaaaaagc aaattttgag ttggaataat aatgacgtcc 901 atttaaatga actgtatatt catccatagt tttatcaaca ggatatcctc caagtgattt 961 ttcacttatt gttgaaggta attttccttc gtatgctata atatcaacaa aatagtttaa 1021 gtttttaggg cggaaagaat acaccgcact aaagtctgcc tcagatgata tatgaactat 1081 ctggagttgt tccagggcga cagattcaaa gcgtgcagtt ctttcctttt caataatttc 1141 actgtatgtt tcatactttg attgcttata gtactcaaag aaactatctc ccctatacca 1201 aacaatcgcc attataaaca aaagaattac gacagctacc cgggaagcaa gaaccttccc 1261 ggtagcgtta tctttgaaca agcgatcgag aacaccaaat agaatatcag agggcgaaaa 1321 tgatattcta ggtgctgcca tagaccctcc ttttaagtac tagagttgtc ccctctttcg 1381 acccatttgc tgtccaccag tcatgctagc catatggctg ccgcgcggca ccaggccgct 1441 gctgtgatga tgatgatgat ggctgctgcc catggtatat ctccttctta aagttaaaca 1501 aaattatttc ttgagcaacc attatcaccg ccagaggtaa aatagtcaac acgcacggtg 1561 ttactctagt atataaacgc agaaaggccc acccgaaggt gagccagtgt gactctagta 1621 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt 1681 tcgttttatt tgatgcctgg ctctagtagc gatctacact agcactatca gcgttattaa 1741 gctactaaag cgtagttttc gtcgtttgca gcgccaaacg tctcttcagg ccactgacta 1801 gcgataactt tccccacaac ggaacaactc tcattgcatg ggatcattgg gtactgtggg 1861 tttagtggtt gtaaaaacac ctgaccgcta tccctgatca gtttcttgaa ggtaaactca 1921 tcacccccaa gtctggctat gcagaaatca cctggctcaa cagcctgctc agggtcaacg 1981 agaattaaca ttccgtcagg aaagctcggc ttggagcctg ttggtgcggt catggaatta 2041 ccttcaacct caagccagaa tgcagaatca ctggcttttt tggttgtgct tacccatctc 2101 tccgcatcac ctttggtaaa ggttctaagc tcaggtgaga acatccctgc ctgaacatga 2161 gaaaaaacag ggtactcata ctcacttcta agtgacggct gcatactaac cgcttcatac 2221 atctcgtaga tttctctggc gattgaaggg ctaaattctt caacgctaac tttgagaatt 2281 tttgcaagca atgcggcgtt ataagcattt aatgcattga tgccattaaa taaagcacca 2341 acgcctgact gccccatccc catcttgtct gcgacagatt cctgggataa gccaagttca 2401 tttttctttt tttcataaat tgctttaagg cgacgtgcgt cctcaagctg ctcttgtgtt 2461 aatggtttct tttttgtgct catctagtat ttctcctctt ttcttgactc cgttgtgatg 2521 acgcattggt acgcggtatc gggaggttcg aaaatttcga gcgatatctt aaggggggtg 2581 ccttacgtag aaccccgtag gtcatgcccg aggccggtcc tggatggcgc ggcggatacg 2641 cttgagcagg ttttcgtcga gaagcggctt caaaaccacg tcttttacgc cggcctcggc 2701 ggcccgggtc gagatgtttt cgtccggata gccggtgatc aggatcacgg gcgtagatct 2761 cgatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg 2821 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga 2881 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca 2941 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg 3001 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg agatcccgga caccatcgaa 3061 tggtgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca attgagggtg 3121 gtgaatgtgg ctagttttca atcatttggg ataccaggac agctggaagt catcaaaaaa 3181 gcacttgatc acgtgcgagt cggtgtggta attacagatc ccgcacttga agataatcct 3241 attgtctacg taaatcaagg ctttgttcaa atgaccggct acgagaccga ggaaatttta 3301 ggaaagaact gtcgcttctt acaggggaaa cacacagatc ctgctgaagt ggacaacatc 3361 agaaccgctt tacaaaataa agaaccggtc accgttcaga tccaaaacta caaaaaagac 3421 ggaacgatgt tctggaatga attaaatatt gatccaatgg aaatagagga taaaacgtat 3481 tttgtcggta ttcagaatga tatcaccgag caccagcaga cccaggcgcg cctccaggaa 3541 ctgcaatccg agctcgtcca cgtctccagg ctgagcgcca tgggcgaaat ggcgtccgcg 3601 ctcgcgcacg agctcaacca gccgctggcg gcgatcagca actacatgaa gggctcgcgg 3661 cggctgcttg ccggcagcag tgatccgaac acaccgaagg tcgaaagcgc cctggaccgc 3721 gccgccgagc aggcgctgcg cgccggccag atcatccggc gcctgcgcga cttcgttgcc 3781 cgcggcgaat cggagaagcg ggtcgagagt ctctccaagc tgatcgagga ggccggcgcg 3841 ctcgggcttg ccggcgcgcg cgagcagaac gtgcagctcc gcttcagtct cgatccgggc 3901 gccgatctcg ttctcgccga ccgggtgcag atccagcagg tcctggtcaa cctgttccgc 3961 aacgcgctgg aagcgatggc tcagtcgcag cgacgcgagc tcgtcgtcac caacaccccc 4021 gccgccgacg acatgatcga ggtcgaagtg tccgacaccg gcagcggttt ccaggacgac 4081 gtcattccga acctgtttca gactttcttc accaccaagg acaccggcat gggcgtggga 4141 ctgtccatca gccgctcgat catcgaagct cacggcgggc gcatgtgggc cgagagcaac 4201 gcatcgggcg gggcgacctt ccgcttcacc ctcccggcag ccgacgagat gataggaggt 4261 ctagcatgac gaccaaggga catatctacg tcatcgacga cgacgcggcg atgcgggatt 4321 cgctgaattt cctgctggat tctgccggct tcggcgtcac gctgtttgac gacgcgcaag 4381 cctttctcga cgccctgccg ggtctctcct tcggctgcgt cgtctccgac gtgcgcatgc 4441 cgggccttga cggcatcgag ctgttgaagc ggatgaaggc gcagcaaagc ccctttccga 4501 tcctcatcat gaccggtcac ggcgacgtgc cgctcgcggt cgaggcgatg aagttagggg 4561 cggtggactt tctggaaaag cctttcgagg acgaccgcct caccgccatg atcgaatcgg 4621 cgatccgcca ggccgagccg gccgccaaga gcgaggccgt cgcgcaggat atcgccgccc 4681 gcgtcgcctc gttgagcccc agggagcgcc aggtcatgga agggctgatc gccggccttt 4741 ccaacaagct gatcgcccgc gagtacgaca tcagcccgcg caccatcgag gtgtatcggg 4801 ccaacgtcat gaccaagatg caggccaaca gcctttcgga gctggttcgc ctcgcgatgc 4861 gcgccggcat gctcaacgat tga //