BBa_K1616022 1 BBa_K1616022 pDawn - Endolysin/Holin 2015-09-17T11:00:00Z 2015-09-18T06:42:39Z This part is composed of Holin/Endolysin toxin BBa_K1616005 and pDawn system BBa_K1616019 Toxins will be produced after light stimulation and induce lysis. false false _2033_ 22805 22805 9 false The original sequence of pDawn have some illegal sites that have been removed. false Johanna Chesnel component2471400 1 BBa_K1616005 component2471406 1 BBa_K1616019 annotation2471406 1 BBa_K1616019 range2471406 1 1380 4883 annotation2471400 1 BBa_K1616005 range2471400 1 1 1379 BBa_K1616019 1 pDawn pDawn system 2015-09-17T11:00:00Z 2015-09-18T06:53:36Z The pDawn & pDusk system, have been optimized and constructed in the way that the toxins sequences we introduce in our construction are in fact the reverse sequences. After reading many papers to select an appropriate light-sensing system to use for the kill switch, our team came across the pDawn and pDusk plasmids. Those plasmids employ bluelight photoreceptors to confer light-repressed or light-induced gene expression in with up to 460-fold induction upon illumination. Controlled by this system, we thought to add toxins in it to kill our bacteria whenever we want(Ohlendorf, Vidavski, Eldar, Moffat, & M??glich, 2012). They are not dependent on nonnative chromophores that are often supplied exogenously and do not need any introduction of cofactor-synthesis genes. Moreover, they are practical to use since current optogenetic tools require multiple plasmid components. Finally, they deal with many limitations in a one-plasmid system which is based on the YF1/FixJ system. false false _2033_ 22805 22805 9 false The original sequence of pDawn have some illegal sites that have been removed. false Johanna Chesnel annotation2470882 1 YF1/FixJ bicistronic operon range2470882 1 2876 3493 annotation2470867 1 cI-LVA range2470867 1 356 1102 annotation2470880 1 YF1 range2470880 1 1743 2876 annotation2470877 1 FixK2 promoter range2470877 1 1125 1372 annotation2470848 1 lambda pR promoter range2470848 1 136 184 BBa_K1616007 1 Holin-rev Holin reversed 2015-09-16T11:00:00Z 2015-09-17T11:02:45Z This sequence is the reverses sequence of the BBa_K112805 (Holin). Holin works with endolysin; they create a toxic system, after a period of late-gene expression, holin, an inner permeabilizing protein creates holes in the membrane in order to permeabilize it and then endolysin, a muralytic enzyme traverses the holes creating by holin and degraded the wall cell. This complex induce cell lysis of bacteria. (Young & Bl??si, 1995) This part is the reversed sequence of the holin (BBa_K112805); thus this part can be used with a reversed endolysin (BBa_K1616006), reversed promoter, reversed RBS and reversed terminator. false false _2033_ 22805 22805 9 false The illegal sites have been checked false Johanna Chesnel annotation2466349 1 Holin range2466349 1 1 676 BBa_K1616011 1 RBS-rev RBS reversed 2015-09-16T11:00:00Z 2015-09-17T10:56:58Z This part is the reverse sequence of the BBa_I712074 promoter T7. For more efficiency of construction, scientists use more and more reversed promoter and then reversed sequences of proteins coding. This part have been created in order to recruit transcriptional machinery and lead to transcritpion of the upstream DNA sequence. So, this part is the reverse sequence of the BBa_I712074 promoter T7. false false _2033_ 22805 22805 9 false We have checked that any illegal sites was formed. false Johanna Chesnel annotation2466086 1 RBS Reversed range2466086 1 1 14 BBa_K1616006 1 End-rev Endolysin reversed 2015-09-16T11:00:00Z 2015-09-17T11:02:11Z This sequence is the reverse sequence of the holin/endolysin system. Holin and endolysin are toxins; after a period of late-gene expression, holin, an inner permeabilizing protein creates holes in the membrane in order to permeabilize it and then endolysin, a muralytic enzyme traverses the holes creating by holin and degraded the wall cell. This complex induce cell lysis of bacteria. (Young & Bl??si, 1995) <br><br> This part is the sequence reverse of the Holin/ Endolysin; thus this part can be used with a reverse promoter. Young, R., & Bl??si, U. (1995). Holins: Form and function in bacteriophage lysis. In FEMS Microbiology Reviews (Vol. 17, pp. 191???205). http://doi.org/10.1016/01686445(94)00079-4 false false _2033_ 22805 22805 9 false The illegal sites have been checked false Johanna Chesnel annotation2466126 1 Endolysin range2466126 1 1 514 BBa_K1616005 1 H/E-rev Holin/endolysin reversed - RBS and double T7 terminator 2015-09-16T11:00:00Z 2015-09-22T07:07:55Z BBa_K1616005 is a composite part with BBa_K1616006 (endolysin reversed), BBa_K1616007 (reversed), BBa_K1616011 (RBS reversed) and BBa_B0025 (Double terminator T7 reversed). Holin and endolysin are toxins; after a period of late-gene expression, holin, an inner permeabilizing protein creates holes in the membrane in order to permeabilize it and then endolysin, a muralytic enzyme traverses the holes creating by holin and degraded the wall cell. This complex induce cell lysis of bacteria. (Young & Bl??si, 1995) false false _2033_ 22805 22805 9 false Check of illegal sites false Johanna Chesnel component2466380 1 BBa_B0025 component2466382 1 BBa_K1616006 component2466388 1 BBa_K1616011 component2466384 1 BBa_K1616011 component2466386 1 BBa_K1616007 annotation2466382 1 BBa_K1616006 range2466382 1 138 651 annotation2466384 1 BBa_K1616011 range2466384 1 660 673 annotation2466380 1 BBa_B0025 range2466380 1 1 129 annotation2466388 1 BBa_K1616011 range2466388 1 1366 1379 annotation2466386 1 BBa_K1616007 range2466386 1 682 1357 BBa_B0025 1 BBa_B0025 double terminator (B0015), reversed 2003-12-02T12:00:00Z 2015-08-31T04:07:20Z -- No description -- false true _1_ 0 24 7 In stock false true Caitlin Conboy annotation369703 1 B0010 range369703 1 50 129 annotation369702 1 B0012 range369702 1 1 41 BBa_K1616006_sequence 1 gctttatagatttttatacgcgtcccaagtgccagttctaaacgttgtaatgactcgttttgcgcgattaggtgtttgattataccatctacttttagctaagttaactgctgcttcatcccagcgtttttgttgaagcatacgtaaagagttagtaaatcctgccacaccggtttctcccatttggaaaaccatattaatcaatgcacagcgacgaaccgcatcaagagaatcataaaccggttttaatttagcatttctcaggattccgcgaacagcagcatcaacatcctgattaaagagtttttcagcctcatcttttgtaattacaccattgcaattacgcccaatagctttatctaattcagatttagcagcattaagtgatggactttttgtaagcaaatgaccgatgccaatagtgtaatagccttctgtgtctttatagattttaagtctaagaccttcatctatacgtaacatttcaaatatattcataatacctcctaagtat BBa_K1616019_sequence 1 gacccatttgctgtccaccagtcatgctagccatatggctgccgcgcggcaccaggccgctgctgtgatgatgatgatgatggctgctgcccatggtatatctccttcttaaagttaaacaaaattatttcttgagcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttactctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagcgatctacactagcactatcagcgttattaagctactaaagcgtagttttcgtcgtttgcagcgccaaacgtctcttcaggccactgactagcgataactttccccacaacggaacaactctcattgcatgggatcattgggtactgtgggtttagtggttgtaaaaacacctgaccgctatccctgatcagtttcttgaaggtaaactcatcacccccaagtctggctatgcagaaatcacctggctcaacagcctgctcagggtcaacgagaattaacattccgtcaggaaagctcggcttggagcctgttggtgcggtcatggaattaccttcaacctcaagccagaatgcagaatcactggcttttttggttgtgcttacccatctctccgcatcacctttggtaaaggttctaagctcaggtgagaacatccctgcctgaacatgagaaaaaacagggtactcatactcacttctaagtgacggctgcatactaaccgcttcatacatctcgtagatttctctggcgattgaagggctaaattcttcaacgctaactttgagaatttttgcaagcaatgcggcgttataagcatttaatgcattgatgccattaaataaagcaccaacgcctgactgccccatccccatcttgtctgcgacagattcctgggataagccaagttcatttttctttttttcataaattgctttaaggcgacgtgcgtcctcaagctgctcttgtgttaatggtttcttttttgtgctcatctagtatttctcctcttttcttgactccgttgtgatgacgcattggtacgcggtatcgggaggttcgaaaatttcgagcgatatcttaaggggggtgccttacgtagaaccccgtaggtcatgcccgaggccggtcctggatggcgcggcggatacgcttgagcaggttttcgtcgagaagcggcttcaaaaccacgtcttttacgccggcctcggcggcccgggtcgagatgttttcgtccggatagccggtgatcaggatcacgggcgtagatctcgatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgagatcccggacaccatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattgagggtggtgaatgtggctagttttcaatcatttgggataccaggacagctggaagtcatcaaaaaagcacttgatcacgtgcgagtcggtgtggtaattacagatcccgcacttgaagataatcctattgtctacgtaaatcaaggctttgttcaaatgaccggctacgagaccgaggaaattttaggaaagaactgtcgcttcttacaggggaaacacacagatcctgctgaagtggacaacatcagaaccgctttacaaaataaagaaccggtcaccgttcagatccaaaactacaaaaaagacggaacgatgttctggaatgaattaaatattgatccaatggaaatagaggataaaacgtattttgtcggtattcagaatgatatcaccgagcaccagcagacccaggcgcgcctccaggaactgcaatccgagctcgtccacgtctccaggctgagcgccatgggcgaaatggcgtccgcgctcgcgcacgagctcaaccagccgctggcggcgatcagcaactacatgaagggctcgcggcggctgcttgccggcagcagtgatccgaacacaccgaaggtcgaaagcgccctggaccgcgccgccgagcaggcgctgcgcgccggccagatcatccggcgcctgcgcgacttcgttgcccgcggcgaatcggagaagcgggtcgagagtctctccaagctgatcgaggaggccggcgcgctcgggcttgccggcgcgcgcgagcagaacgtgcagctccgcttcagtctcgatccgggcgccgatctcgttctcgccgaccgggtgcagatccagcaggtcctggtcaacctgttccgcaacgcgctggaagcgatggctcagtcgcagcgacgcgagctcgtcgtcaccaacacccccgccgccgacgacatgatcgaggtcgaagtgtccgacaccggcagcggtttccaggacgacgtcattccgaacctgtttcagactttcttcaccaccaaggacaccggcatgggcgtgggactgtccatcagccgctcgatcatcgaagctcacggcgggcgcatgtgggccgagagcaacgcatcgggcggggcgaccttccgcttcaccctcccggcagccgacgagatgataggaggtctagcatgacgaccaagggacatatctacgtcatcgacgacgacgcggcgatgcgggattcgctgaatttcctgctggattctgccggcttcggcgtcacgctgtttgacgacgcgcaagcctttctcgacgccctgccgggtctctccttcggctgcgtcgtctccgacgtgcgcatgccgggccttgacggcatcgagctgttgaagcggatgaaggcgcagcaaagcccctttccgatcctcatcatgaccggtcacggcgacgtgccgctcgcggtcgaggcgatgaagttaggggcggtggactttctggaaaagcctttcgaggacgaccgcctcaccgccatgatcgaatcggcgatccgccaggccgagccggccgccaagagcgaggccgtcgcgcaggatatcgccgcccgcgtcgcctcgttgagccccagggagcgccaggtcatggaagggctgatcgccggcctttccaacaagctgatcgcccgcgagtacgacatcagcccgcgcaccatcgaggtgtatcgggccaacgtcatgaccaagatgcaggccaacagcctttcggagctggttcgcctcgcgatgcgcgccggcatgctcaacgattga BBa_K1616011_sequence 1 ttgtcccctctttc BBa_K1616022_sequence 1 tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtactagaggctttatagatttttatacgcgtcccaagtgccagttctaaacgttgtaatgactcgttttgcgcgattaggtgtttgattataccatctacttttagctaagttaactgctgcttcatcccagcgtttttgttgaagcatacgtaaagagttagtaaatcctgccacaccggtttctcccatttggaaaaccatattaatcaatgcacagcgacgaaccgcatcaagagaatcataaaccggttttaatttagcatttctcaggattccgcgaacagcagcatcaacatcctgattaaagagtttttcagcctcatcttttgtaattacaccattgcaattacgcccaatagctttatctaattcagatttagcagcattaagtgatggactttttgtaagcaaatgaccgatgccaatagtgtaatagccttctgtgtctttatagattttaagtctaagaccttcatctatacgtaacatttcaaatatattcataatacctcctaagtattactagagttgtcccctctttctactagagtaattatttagcccttcctaatattctggccgcctgagcacatattgattcaaggcggtcattacttatatgatcatttctataccagtacatggttatcgttccagcatagatattatccaaattaaaatatggacaactgtacatgtagtttatttcgggagtaggctttttagttggtaaaaaagcaaattttgagttggaataataatgacgtccatttaaatgaactgtatattcatccatagttttatcaacaggatatcctccaagtgatttttcacttattgttgaaggtaattttccttcgtatgctataatatcaacaaaatagtttaagtttttagggcggaaagaatacaccgcactaaagtctgcctcagatgatatatgaactatctggagttgttccagggcgacagattcaaagcgtgcagttctttccttttcaataatttcactgtatgtttcatactttgattgcttatagtactcaaagaaactatctcccctataccaaacaatcgccattataaacaaaagaattacgacagctacccgggaagcaagaaccttcccggtagcgttatctttgaacaagcgatcgagaacaccaaatagaatatcagagggcgaaaatgatattctaggtgctgccatagaccctccttttaagtactagagttgtcccctctttcgacccatttgctgtccaccagtcatgctagccatatggctgccgcgcggcaccaggccgctgctgtgatgatgatgatgatggctgctgcccatggtatatctccttcttaaagttaaacaaaattatttcttgagcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttactctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagcgatctacactagcactatcagcgttattaagctactaaagcgtagttttcgtcgtttgcagcgccaaacgtctcttcaggccactgactagcgataactttccccacaacggaacaactctcattgcatgggatcattgggtactgtgggtttagtggttgtaaaaacacctgaccgctatccctgatcagtttcttgaaggtaaactcatcacccccaagtctggctatgcagaaatcacctggctcaacagcctgctcagggtcaacgagaattaacattccgtcaggaaagctcggcttggagcctgttggtgcggtcatggaattaccttcaacctcaagccagaatgcagaatcactggcttttttggttgtgcttacccatctctccgcatcacctttggtaaaggttctaagctcaggtgagaacatccctgcctgaacatgagaaaaaacagggtactcatactcacttctaagtgacggctgcatactaaccgcttcatacatctcgtagatttctctggcgattgaagggctaaattcttcaacgctaactttgagaatttttgcaagcaatgcggcgttataagcatttaatgcattgatgccattaaataaagcaccaacgcctgactgccccatccccatcttgtctgcgacagattcctgggataagccaagttcatttttctttttttcataaattgctttaaggcgacgtgcgtcctcaagctgctcttgtgttaatggtttcttttttgtgctcatctagtatttctcctcttttcttgactccgttgtgatgacgcattggtacgcggtatcgggaggttcgaaaatttcgagcgatatcttaaggggggtgccttacgtagaaccccgtaggtcatgcccgaggccggtcctggatggcgcggcggatacgcttgagcaggttttcgtcgagaagcggcttcaaaaccacgtcttttacgccggcctcggcggcccgggtcgagatgttttcgtccggatagccggtgatcaggatcacgggcgtagatctcgatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgagatcccggacaccatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattgagggtggtgaatgtggctagttttcaatcatttgggataccaggacagctggaagtcatcaaaaaagcacttgatcacgtgcgagtcggtgtggtaattacagatcccgcacttgaagataatcctattgtctacgtaaatcaaggctttgttcaaatgaccggctacgagaccgaggaaattttaggaaagaactgtcgcttcttacaggggaaacacacagatcctgctgaagtggacaacatcagaaccgctttacaaaataaagaaccggtcaccgttcagatccaaaactacaaaaaagacggaacgatgttctggaatgaattaaatattgatccaatggaaatagaggataaaacgtattttgtcggtattcagaatgatatcaccgagcaccagcagacccaggcgcgcctccaggaactgcaatccgagctcgtccacgtctccaggctgagcgccatgggcgaaatggcgtccgcgctcgcgcacgagctcaaccagccgctggcggcgatcagcaactacatgaagggctcgcggcggctgcttgccggcagcagtgatccgaacacaccgaaggtcgaaagcgccctggaccgcgccgccgagcaggcgctgcgcgccggccagatcatccggcgcctgcgcgacttcgttgcccgcggcgaatcggagaagcgggtcgagagtctctccaagctgatcgaggaggccggcgcgctcgggcttgccggcgcgcgcgagcagaacgtgcagctccgcttcagtctcgatccgggcgccgatctcgttctcgccgaccgggtgcagatccagcaggtcctggtcaacctgttccgcaacgcgctggaagcgatggctcagtcgcagcgacgcgagctcgtcgtcaccaacacccccgccgccgacgacatgatcgaggtcgaagtgtccgacaccggcagcggtttccaggacgacgtcattccgaacctgtttcagactttcttcaccaccaaggacaccggcatgggcgtgggactgtccatcagccgctcgatcatcgaagctcacggcgggcgcatgtgggccgagagcaacgcatcgggcggggcgaccttccgcttcaccctcccggcagccgacgagatgataggaggtctagcatgacgaccaagggacatatctacgtcatcgacgacgacgcggcgatgcgggattcgctgaatttcctgctggattctgccggcttcggcgtcacgctgtttgacgacgcgcaagcctttctcgacgccctgccgggtctctccttcggctgcgtcgtctccgacgtgcgcatgccgggccttgacggcatcgagctgttgaagcggatgaaggcgcagcaaagcccctttccgatcctcatcatgaccggtcacggcgacgtgccgctcgcggtcgaggcgatgaagttaggggcggtggactttctggaaaagcctttcgaggacgaccgcctcaccgccatgatcgaatcggcgatccgccaggccgagccggccgccaagagcgaggccgtcgcgcaggatatcgccgcccgcgtcgcctcgttgagccccagggagcgccaggtcatggaagggctgatcgccggcctttccaacaagctgatcgcccgcgagtacgacatcagcccgcgcaccatcgaggtgtatcgggccaacgtcatgaccaagatgcaggccaacagcctttcggagctggttcgcctcgcgatgcgcgccggcatgctcaacgattga BBa_K1616007_sequence 1 taattatttagcccttcctaatattctggccgcctgagcacatattgattcaaggcggtcattacttatatgatcatttctataccagtacatggttatcgttccagcatagatattatccaaattaaaatatggacaactgtacatgtagtttatttcgggagtaggctttttagttggtaaaaaagcaaattttgagttggaataataatgacgtccatttaaatgaactgtatattcatccatagttttatcaacaggatatcctccaagtgatttttcacttattgttgaaggtaattttccttcgtatgctataatatcaacaaaatagtttaagtttttagggcggaaagaatacaccgcactaaagtctgcctcagatgatatatgaactatctggagttgttccagggcgacagattcaaagcgtgcagttctttccttttcaataatttcactgtatgtttcatactttgattgcttatagtactcaaagaaactatctcccctataccaaacaatcgccattataaacaaaagaattacgacagctacccgggaagcaagaaccttcccggtagcgttatctttgaacaagcgatcgagaacaccaaatagaatatcagagggcgaaaatgatattctaggtgctgccatagaccctccttttaag BBa_B0025_sequence 1 tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctgg BBa_K1616005_sequence 1 tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtactagaggctttatagatttttatacgcgtcccaagtgccagttctaaacgttgtaatgactcgttttgcgcgattaggtgtttgattataccatctacttttagctaagttaactgctgcttcatcccagcgtttttgttgaagcatacgtaaagagttagtaaatcctgccacaccggtttctcccatttggaaaaccatattaatcaatgcacagcgacgaaccgcatcaagagaatcataaaccggttttaatttagcatttctcaggattccgcgaacagcagcatcaacatcctgattaaagagtttttcagcctcatcttttgtaattacaccattgcaattacgcccaatagctttatctaattcagatttagcagcattaagtgatggactttttgtaagcaaatgaccgatgccaatagtgtaatagccttctgtgtctttatagattttaagtctaagaccttcatctatacgtaacatttcaaatatattcataatacctcctaagtattactagagttgtcccctctttctactagagtaattatttagcccttcctaatattctggccgcctgagcacatattgattcaaggcggtcattacttatatgatcatttctataccagtacatggttatcgttccagcatagatattatccaaattaaaatatggacaactgtacatgtagtttatttcgggagtaggctttttagttggtaaaaaagcaaattttgagttggaataataatgacgtccatttaaatgaactgtatattcatccatagttttatcaacaggatatcctccaagtgatttttcacttattgttgaaggtaattttccttcgtatgctataatatcaacaaaatagtttaagtttttagggcggaaagaatacaccgcactaaagtctgcctcagatgatatatgaactatctggagttgttccagggcgacagattcaaagcgtgcagttctttccttttcaataatttcactgtatgtttcatactttgattgcttatagtactcaaagaaactatctcccctataccaaacaatcgccattataaacaaaagaattacgacagctacccgggaagcaagaaccttcccggtagcgttatctttgaacaagcgatcgagaacaccaaatagaatatcagagggcgaaaatgatattctaggtgctgccatagaccctccttttaagtactagagttgtcccctctttc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z