BBa_K1616022
type: ComponentDefinition
persistent identity: BBa K1616022
display id: BBa_K1616022
version: 1
was generated by: iGEM to SBOL conversion
description: pDawn - Endolysin/Holin
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T06:42:39Z
mutable provenance: This part is composed of Holin/Endolysin toxin BBa_K1616005 and pDawn system BBa_K1616019
owned by: james
owned by: myers
mutable description: Toxins will be produced after light stimulation and induce lysis.
discontinued: false
dominant: false
group u list: _2033_
m user id: 22805
owner id: 22805
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The original sequence of pDawn have some illegal sites that have been removed.
star: false
creator: Johanna Chesnel
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1616005
component: BBa_K1616019
sequence annotation: BBa_K1616005
sequence annotation: BBa_K1616019
sequence: https://synbiohub.org/public/igem/BBa_K1616022_sequence/1

BBa_K1616005
type: Component
persistent identity: component2471400
display id: component2471400
version: 1
top level: BBa_K1616022
definition: H/E-rev
access: public

BBa_K1616019
type: Component
persistent identity: component2471406
display id: component2471406
version: 1
top level: BBa_K1616022
definition: pDawn
access: public

BBa_K1616005
type: SequenceAnnotation
persistent identity: annotation2471400
display id: annotation2471400
version: 1
top level: BBa_K1616022
location: https://synbiohub.org/public/igem/BBa_K1616022/annotation2471400/range2471400/1
component: BBa_K1616005

https://synbiohub.org/public/igem/BBa_K1616022/annotation2471400/range2471400/1
type: Range
persistent identity: range2471400
display id: range2471400
version: 1
top level: BBa_K1616022
direction: direction/reverse
start: 1
end: 1379
orientation: inline

BBa_K1616019
type: SequenceAnnotation
persistent identity: annotation2471406
display id: annotation2471406
version: 1
top level: BBa_K1616022
location: https://synbiohub.org/public/igem/BBa_K1616022/annotation2471406/range2471406/1
component: BBa_K1616019

https://synbiohub.org/public/igem/BBa_K1616022/annotation2471406/range2471406/1
type: Range
persistent identity: range2471406
display id: range2471406
version: 1
top level: BBa_K1616022
direction: direction/reverse
start: 1380
end: 4883
orientation: inline

H/E-rev
type: ComponentDefinition
persistent identity: BBa K1616005
display id: BBa_K1616005
version: 1
was generated by: iGEM to SBOL conversion
description: Holin/endolysin reversed - RBS and double T7 terminator
created: 2015-09-16T11:00:00Z
modified: 2015-09-22T07:07:55Z
mutable provenance: BBa_K1616005 is a composite part with BBa_K1616006 (endolysin reversed), BBa_K1616007 (reversed), BBa_K1616011 (RBS reversed) and BBa_B0025 (Double terminator T7 reversed).
owned by: james
owned by: myers
mutable description: Holin and endolysin are toxins; after a period of late-gene expression, holin, an inner permeabilizing protein creates holes in the membrane in order to permeabilize it and then endolysin, a muralytic enzyme traverses the holes creating by holin and degraded the wall cell. This complex induce cell lysis of bacteria. (Young & Bl??si, 1995)
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2033_
m user id: 22805
owner id: 22805
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Check of illegal sites
star: false
creator: Johanna Chesnel
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1616011
component: BBa_K1616006
component: BBa_K1616007
component: BBa_B0025
sequence annotation: BBa_B0025
sequence annotation: BBa_K1616006
sequence annotation: BBa_K1616011
sequence annotation: BBa_K1616007
sequence: https://synbiohub.org/public/igem/BBa_K1616005_sequence/1

BBa_K1616011
type: Component
persistent identity: component2466384
display id: component2466384
version: 1
top level: H/E-rev
definition: RBS-rev
access: public

BBa_K1616006
type: Component
persistent identity: component2466382
display id: component2466382
version: 1
top level: H/E-rev
definition: End-rev
access: public

BBa_K1616011
type: Component
persistent identity: component2466388
display id: component2466388
version: 1
top level: H/E-rev
definition: RBS-rev
access: public

BBa_K1616007
type: Component
persistent identity: component2466386
display id: component2466386
version: 1
top level: H/E-rev
definition: Holin-rev
access: public

BBa_B0025
type: Component
persistent identity: component2466380
display id: component2466380
version: 1
top level: H/E-rev
definition: BBa_B0025
access: public

BBa_B0025
type: SequenceAnnotation
persistent identity: annotation2466380
display id: annotation2466380
version: 1
top level: H/E-rev
location: https://synbiohub.org/public/igem/BBa_K1616005/annotation2466380/range2466380/1
component: BBa_B0025

https://synbiohub.org/public/igem/BBa_K1616005/annotation2466380/range2466380/1
type: Range
persistent identity: range2466380
display id: range2466380
version: 1
top level: H/E-rev
direction: direction/reverse
start: 1
end: 129
orientation: inline

BBa_K1616006
type: SequenceAnnotation
persistent identity: annotation2466382
display id: annotation2466382
version: 1
top level: H/E-rev
location: https://synbiohub.org/public/igem/BBa_K1616005/annotation2466382/range2466382/1
component: BBa_K1616006

https://synbiohub.org/public/igem/BBa_K1616005/annotation2466382/range2466382/1
type: Range
persistent identity: range2466382
display id: range2466382
version: 1
top level: H/E-rev
direction: direction/reverse
start: 138
end: 651
orientation: inline

BBa_K1616011
type: SequenceAnnotation
persistent identity: annotation2466384
display id: annotation2466384
version: 1
top level: H/E-rev
location: https://synbiohub.org/public/igem/BBa_K1616005/annotation2466384/range2466384/1
component: BBa_K1616011

https://synbiohub.org/public/igem/BBa_K1616005/annotation2466384/range2466384/1
type: Range
persistent identity: range2466384
display id: range2466384
version: 1
top level: H/E-rev
direction: direction/reverse
start: 660
end: 673
orientation: inline

BBa_K1616007
type: SequenceAnnotation
persistent identity: annotation2466386
display id: annotation2466386
version: 1
top level: H/E-rev
location: https://synbiohub.org/public/igem/BBa_K1616005/annotation2466386/range2466386/1
component: BBa_K1616007

https://synbiohub.org/public/igem/BBa_K1616005/annotation2466386/range2466386/1
type: Range
persistent identity: range2466386
display id: range2466386
version: 1
top level: H/E-rev
direction: direction/reverse
start: 682
end: 1357
orientation: inline

BBa_K1616011
type: SequenceAnnotation
persistent identity: annotation2466388
display id: annotation2466388
version: 1
top level: H/E-rev
location: https://synbiohub.org/public/igem/BBa_K1616005/annotation2466388/range2466388/1
component: BBa_K1616011

https://synbiohub.org/public/igem/BBa_K1616005/annotation2466388/range2466388/1
type: Range
persistent identity: range2466388
display id: range2466388
version: 1
top level: H/E-rev
direction: direction/reverse
start: 1366
end: 1379
orientation: inline

pDawn
type: ComponentDefinition
persistent identity: BBa K1616019
display id: BBa_K1616019
version: 1
was generated by: iGEM to SBOL conversion
description: pDawn system
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T06:53:36Z
mutable provenance: The pDawn & pDusk system, have been optimized and constructed in the way that the toxins sequences we introduce in our construction are in fact the reverse sequences.
owned by: james
owned by: myers
mutable description: After reading many papers to select an appropriate light-sensing system to use for the kill switch, our team came across the pDawn and pDusk plasmids. Those plasmids employ bluelight photoreceptors to confer light-repressed or light-induced gene expression in with up to 460-fold induction upon illumination. Controlled by this system, we thought to add toxins in it to kill our bacteria whenever we want(Ohlendorf, Vidavski, Eldar, Moffat, & M??glich, 2012). They are not dependent on nonnative chromophores that are often supplied exogenously and do not need any introduction of cofactor-synthesis genes. Moreover, they are practical to use since current optogenetic tools require multiple plasmid components. Finally, they deal with many limitations in a one-plasmid system which is based on the YF1/FixJ system.
discontinued: false
dominant: false
experience: experience/None
group u list: _2033_
m user id: 22805
owner id: 22805
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The original sequence of pDawn have some illegal sites that have been removed.
star: false
creator: Johanna Chesnel
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lambda pR promoter
sequence annotation: cI-LVA
sequence annotation: FixK2 promoter
sequence annotation: YF1
sequence annotation: YF1/FixJ bicistronic operon
sequence: https://synbiohub.org/public/igem/BBa_K1616019_sequence/1

lambda pR promoter
type: SequenceAnnotation
persistent identity: annotation2470848
display id: annotation2470848
version: 1
top level: pDawn
location: https://synbiohub.org/public/igem/BBa_K1616019/annotation2470848/range2470848/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K1616019/annotation2470848/range2470848/1
type: Range
persistent identity: range2470848
display id: range2470848
version: 1
top level: pDawn
direction: direction/reverse
start: 136
end: 184
orientation: inline

cI-LVA
type: SequenceAnnotation
persistent identity: annotation2470867
display id: annotation2470867
version: 1
top level: pDawn
location: https://synbiohub.org/public/igem/BBa_K1616019/annotation2470867/range2470867/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1616019/annotation2470867/range2470867/1
type: Range
persistent identity: range2470867
display id: range2470867
version: 1
top level: pDawn
direction: direction/forward
start: 356
end: 1102
orientation: inline

FixK2 promoter
type: SequenceAnnotation
persistent identity: annotation2470877
display id: annotation2470877
version: 1
top level: pDawn
location: https://synbiohub.org/public/igem/BBa_K1616019/annotation2470877/range2470877/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K1616019/annotation2470877/range2470877/1
type: Range
persistent identity: range2470877
display id: range2470877
version: 1
top level: pDawn
direction: direction/forward
start: 1125
end: 1372
orientation: inline

YF1
type: SequenceAnnotation
persistent identity: annotation2470880
display id: annotation2470880
version: 1
top level: pDawn
location: https://synbiohub.org/public/igem/BBa_K1616019/annotation2470880/range2470880/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1616019/annotation2470880/range2470880/1
type: Range
persistent identity: range2470880
display id: range2470880
version: 1
top level: pDawn
direction: direction/forward
start: 1743
end: 2876
orientation: inline

YF1/FixJ bicistronic operon
type: SequenceAnnotation
persistent identity: annotation2470882
display id: annotation2470882
version: 1
top level: pDawn
location: https://synbiohub.org/public/igem/BBa_K1616019/annotation2470882/range2470882/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1616019/annotation2470882/range2470882/1
type: Range
persistent identity: range2470882
display id: range2470882
version: 1
top level: pDawn
direction: direction/forward
start: 2876
end: 3493
orientation: inline

BBa_B0025
type: ComponentDefinition
persistent identity: BBa B0025
display id: BBa_B0025
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0015), reversed
created: 2003-12-02T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B0012
sequence annotation: B0010
sequence: https://synbiohub.org/public/igem/BBa_B0025_sequence/1

B0012
type: SequenceAnnotation
persistent identity: annotation369702
display id: annotation369702
version: 1
top level: BBa_B0025
location: https://synbiohub.org/public/igem/BBa_B0025/annotation369702/range369702/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0025/annotation369702/range369702/1
type: Range
persistent identity: range369702
display id: range369702
version: 1
top level: BBa_B0025
direction: direction/reverse
start: 1
end: 41
orientation: inline

B0010
type: SequenceAnnotation
persistent identity: annotation369703
display id: annotation369703
version: 1
top level: BBa_B0025
location: https://synbiohub.org/public/igem/BBa_B0025/annotation369703/range369703/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0025/annotation369703/range369703/1
type: Range
persistent identity: range369703
display id: range369703
version: 1
top level: BBa_B0025
direction: direction/reverse
start: 50
end: 129
orientation: inline

RBS-rev
type: ComponentDefinition
persistent identity: BBa K1616011
display id: BBa_K1616011
version: 1
was generated by: iGEM to SBOL conversion
description: RBS reversed
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T10:56:58Z
mutable provenance: This part is the reverse sequence of the BBa_I712074 promoter T7.
owned by: james
owned by: myers
mutable description: For more efficiency of construction, scientists use more and more reversed promoter and then reversed sequences of proteins coding. This part have been created in order to recruit transcriptional machinery and lead to transcritpion of the upstream DNA sequence. So, this part is the reverse sequence of the BBa_I712074 promoter T7.
discontinued: false
dominant: false
experience: experience/None
group u list: _2033_
m user id: 22805
owner id: 22805
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We have checked that any illegal sites was formed.
star: false
creator: Johanna Chesnel
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS Reversed
sequence: https://synbiohub.org/public/igem/BBa_K1616011_sequence/1

RBS Reversed
type: SequenceAnnotation
persistent identity: annotation2466086
display id: annotation2466086
version: 1
top level: RBS-rev
location: https://synbiohub.org/public/igem/BBa_K1616011/annotation2466086/range2466086/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1616011/annotation2466086/range2466086/1
type: Range
persistent identity: range2466086
display id: range2466086
version: 1
top level: RBS-rev
direction: direction/reverse
start: 1
end: 14
orientation: inline

End-rev
type: ComponentDefinition
persistent identity: BBa K1616006
display id: BBa_K1616006
version: 1
was generated by: iGEM to SBOL conversion
description: Endolysin reversed
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T11:02:11Z
mutable provenance: This sequence is the reverse sequence of the holin/endolysin system.
owned by: james
owned by: myers
mutable description: Holin and endolysin are toxins; after a period of late-gene expression, holin, an inner permeabilizing protein creates holes in the membrane in order to permeabilize it and then endolysin, a muralytic enzyme traverses the holes creating by holin and degraded the wall cell. This complex induce cell lysis of bacteria. (Young & Bl??si, 1995) This part is the sequence reverse of the Holin/ Endolysin; thus this part can be used with a reverse promoter. Young, R., & Bl??si, U. (1995). Holins: Form and function in bacteriophage lysis. In FEMS Microbiology Reviews (Vol. 17, pp. 191???205). http://doi.org/10.1016/01686445(94)00079-4
discontinued: false
dominant: false
experience: experience/None
group u list: _2033_
m user id: 22805
owner id: 22805
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The illegal sites have been checked
star: false
creator: Johanna Chesnel
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Endolysin
sequence: https://synbiohub.org/public/igem/BBa_K1616006_sequence/1

Endolysin
type: SequenceAnnotation
persistent identity: annotation2466126
display id: annotation2466126
version: 1
top level: End-rev
location: https://synbiohub.org/public/igem/BBa_K1616006/annotation2466126/range2466126/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1616006/annotation2466126/range2466126/1
type: Range
persistent identity: range2466126
display id: range2466126
version: 1
top level: End-rev
direction: direction/reverse
start: 1
end: 514
orientation: inline

Holin-rev
type: ComponentDefinition
persistent identity: BBa K1616007
display id: BBa_K1616007
version: 1
was generated by: iGEM to SBOL conversion
description: Holin reversed
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T11:02:45Z
mutable provenance: This sequence is the reverses sequence of the BBa_K112805 (Holin).
owned by: james
owned by: myers
mutable description: Holin works with endolysin; they create a toxic system, after a period of late-gene expression, holin, an inner permeabilizing protein creates holes in the membrane in order to permeabilize it and then endolysin, a muralytic enzyme traverses the holes creating by holin and degraded the wall cell. This complex induce cell lysis of bacteria. (Young & Bl??si, 1995) This part is the reversed sequence of the holin (BBa_K112805); thus this part can be used with a reversed endolysin (BBa_K1616006), reversed promoter, reversed RBS and reversed terminator.
discontinued: false
dominant: false
experience: experience/None
group u list: _2033_
m user id: 22805
owner id: 22805
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The illegal sites have been checked
star: false
creator: Johanna Chesnel
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Holin
sequence: https://synbiohub.org/public/igem/BBa_K1616007_sequence/1

Holin
type: SequenceAnnotation
persistent identity: annotation2466349
display id: annotation2466349
version: 1
top level: Holin-rev
location: https://synbiohub.org/public/igem/BBa_K1616007/annotation2466349/range2466349/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1616007/annotation2466349/range2466349/1
type: Range
persistent identity: range2466349
display id: range2466349
version: 1
top level: Holin-rev
direction: direction/reverse
start: 1
end: 676
orientation: inline

https://synbiohub.org/public/igem/BBa_K1616022_sequence/1
type: Sequence
persistent identity: BBa K1616022 sequence
display id: BBa_K1616022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtactagaggctttatagatttttatacgcgtcccaagtgccagttctaaacgttgtaatgactcgttttgcgcgattaggtgtttgattataccatctacttttagctaagttaactgctgcttcatcccagcgtttttgttgaagcatacgtaaagagttagtaaatcctgccacaccggtttctcccatttggaaaaccatattaatcaatgcacagcgacgaaccgcatcaagagaatcataaaccggttttaatttagcatttctcaggattccgcgaacagcagcatcaacatcctgattaaagagtttttcagcctcatcttttgtaattacaccattgcaattacgcccaatagctttatctaattcagatttagcagcattaagtgatggactttttgtaagcaaatgaccgatgccaatagtgtaatagccttctgtgtctttatagattttaagtctaagaccttcatctatacgtaacatttcaaatatattcataatacctcctaagtattactagagttgtcccctctttctactagagtaattatttagcccttcctaatattctggccgcctgagcacatattgattcaaggcggtcattacttatatgatcatttctataccagtacatggttatcgttccagcatagatattatccaaattaaaatatggacaactgtacatgtagtttatttcgggagtaggctttttagttggtaaaaaagcaaattttgagttggaataataatgacgtccatttaaatgaactgtatattcatccatagttttatcaacaggatatcctccaagtgatttttcacttattgttgaaggtaattttccttcgtatgctataatatcaacaaaatagtttaagtttttagggcggaaagaatacaccgcactaaagtctgcctcagatgatatatgaactatctggagttgttccagggcgacagattcaaagcgtgcagttctttccttttcaataatttcactgtatgtttcatactttgattgcttatagtactcaaagaaactatctcccctataccaaacaatcgccattataaacaaaagaattacgacagctacccgggaagcaagaaccttcccggtagcgttatctttgaacaagcgatcgagaacaccaaatagaatatcagagggcgaaaatgatattctaggtgctgccatagaccctccttttaagtactagagttgtcccctctttcgacccatttgctgtccaccagtcatgctagccatatggctgccgcgcggcaccaggccgctgctgtgatgatgatgatgatggctgctgcccatggtatatctccttcttaaagttaaacaaaattatttcttgagcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttactctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagcgatctacactagcactatcagcgttattaagctactaaagcgtagttttcgtcgtttgcagcgccaaacgtctcttcaggccactgactagcgataactttccccacaacggaacaactctcattgcatgggatcattgggtactgtgggtttagtggttgtaaaaacacctgaccgctatccctgatcagtttcttgaaggtaaactcatcacccccaagtctggctatgcagaaatcacctggctcaacagcctgctcagggtcaacgagaattaacattccgtcagg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1616005_sequence/1
type: Sequence
persistent identity: BBa K1616005 sequence
display id: BBa_K1616005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtactagaggctttatagatttttatacgcgtcccaagtgccagttctaaacgttgtaatgactcgttttgcgcgattaggtgtttgattataccatctacttttagctaagttaactgctgcttcatcccagcgtttttgttgaagcatacgtaaagagttagtaaatcctgccacaccggtttctcccatttggaaaaccatattaatcaatgcacagcgacgaaccgcatcaagagaatcataaaccggttttaatttagcatttctcaggattccgcgaacagcagcatcaacatcctgattaaagagtttttcagcctcatcttttgtaattacaccattgcaattacgcccaatagctttatctaattcagatttagcagcattaagtgatggactttttgtaagcaaatgaccgatgccaatagtgtaatagccttctgtgtctttatagattttaagtctaagaccttcatctatacgtaacatttcaaatatattcataatacctcctaagtattactagagttgtcccctctttctactagagtaattatttagcccttcctaatattctggccgcctgagcacatattgattcaaggcggtcattacttatatgatcatttctataccagtacatggttatcgttccagcatagatattatccaaattaaaatatggacaactgtacatgtagtttatttcgggagtaggctttttagttggtaaaaaagcaaattttgagttggaataataatgacgtccatttaaatgaactgtatattcatccatagttttatcaacaggatatcctccaagtgatttttcacttattgttgaaggtaattttccttcgtatgctataatatcaacaaaatagtttaagtttttagggcggaaagaatacaccgcactaaagtctgcctcagatgatatatgaactatctggagttgttccagggcgacagattcaaagcgtgcagttctttccttttcaataatttcactgtatgtttcatactttgattgcttatagtactcaaagaaactatctcccctataccaaacaatcgccattataaacaaaagaattacgacagctacccgggaagcaagaaccttcccggtagcgttatctttgaacaagcgatcgagaacaccaaatagaatatcagagggcgaaaatgatattctaggtgctgccatagaccctccttttaagtactagagttgtcccctctttc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1616019_sequence/1
type: Sequence
persistent identity: BBa K1616019 sequence
display id: BBa_K1616019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gacccatttgctgtccaccagtcatgctagccatatggctgccgcgcggcaccaggccgctgctgtgatgatgatgatgatggctgctgcccatggtatatctccttcttaaagttaaacaaaattatttcttgagcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttactctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagcgatctacactagcactatcagcgttattaagctactaaagcgtagttttcgtcgtttgcagcgccaaacgtctcttcaggccactgactagcgataactttccccacaacggaacaactctcattgcatgggatcattgggtactgtgggtttagtggttgtaaaaacacctgaccgctatccctgatcagtttcttgaaggtaaactcatcacccccaagtctggctatgcagaaatcacctggctcaacagcctgctcagggtcaacgagaattaacattccgtcaggaaagctcggcttggagcctgttggtgcggtcatggaattaccttcaacctcaagccagaatgcagaatcactggcttttttggttgtgcttacccatctctccgcatcacctttggtaaaggttctaagctcaggtgagaacatccctgcctgaacatgagaaaaaacagggtactcatactcacttctaagtgacggctgcatactaaccgcttcatacatctcgtagatttctctggcgattgaagggctaaattcttcaacgctaactttgagaatttttgcaagcaatgcggcgttataagcatttaatgcattgatgccattaaataaagcaccaacgcctgactgccccatccccatcttgtctgcgacagattcctgggataagccaagttcatttttctttttttcataaattgctttaaggcgacgtgcgtcctcaagctgctcttgtgttaatggtttcttttttgtgctcatctagtatttctcctcttttcttgactccgttgtgatgacgcattggtacgcggtatcgggaggttcgaaaatttcgagcgatatcttaaggggggtgccttacgtagaaccccgtaggtcatgcccgaggccggtcctggatggcgcggcggatacgcttgagcaggttttcgtcgagaagcggcttcaaaaccacgtcttttacgccggcctcggcggcccgggtcgagatgttttcgtccggatagccggtgatcaggatcacgggcgtagatctcgatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgagatcccggacaccatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattgagggtggtgaatgtggctagttttcaatcatttgggataccaggacagctggaagtcatcaaaaaagcacttgatcacgtgcgagtcggtgtggtaattacagatcccgcacttgaagataatcctattgtctacgtaaatcaaggctttgttcaaatgaccggctacgagaccgaggaaattttaggaaagaactgtcgcttcttacaggggaaacacacagatcctgctgaagtggacaacatcagaaccgctttacaaaata…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0025_sequence/1
type: Sequence
persistent identity: BBa B0025 sequence
display id: BBa_B0025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1616011_sequence/1
type: Sequence
persistent identity: BBa K1616011 sequence
display id: BBa_K1616011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgtcccctctttc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1616006_sequence/1
type: Sequence
persistent identity: BBa K1616006 sequence
display id: BBa_K1616006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctttatagatttttatacgcgtcccaagtgccagttctaaacgttgtaatgactcgttttgcgcgattaggtgtttgattataccatctacttttagctaagttaactgctgcttcatcccagcgtttttgttgaagcatacgtaaagagttagtaaatcctgccacaccggtttctcccatttggaaaaccatattaatcaatgcacagcgacgaaccgcatcaagagaatcataaaccggttttaatttagcatttctcaggattccgcgaacagcagcatcaacatcctgattaaagagtttttcagcctcatcttttgtaattacaccattgcaattacgcccaatagctttatctaattcagatttagcagcattaagtgatggactttttgtaagcaaatgaccgatgccaatagtgtaatagccttctgtgtctttatagattttaagtctaagaccttcatctatacgtaacatttcaaatatattcataatacctcctaagtat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1616007_sequence/1
type: Sequence
persistent identity: BBa K1616007 sequence
display id: BBa_K1616007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taattatttagcccttcctaatattctggccgcctgagcacatattgattcaaggcggtcattacttatatgatcatttctataccagtacatggttatcgttccagcatagatattatccaaattaaaatatggacaactgtacatgtagtttatttcgggagtaggctttttagttggtaaaaaagcaaattttgagttggaataataatgacgtccatttaaatgaactgtatattcatccatagttttatcaacaggatatcctccaagtgatttttcacttattgttgaaggtaattttccttcgtatgctataatatcaacaaaatagtttaagtttttagggcggaaagaatacaccgcactaaagtctgcctcagatgatatatgaactatctggagttgttccagggcgacagattcaaagcgtgcagttctttccttttcaataatttcactgtatgtttcatactttgattgcttatagtactcaaagaaactatctcccctataccaaacaatcgccattataaacaaaagaattacgacagctacccgggaagcaagaaccttcccggtagcgttatctttgaacaagcgatcgagaacaccaaatagaatatcagagggcgaaaatgatattctaggtgctgccatagaccctccttttaag
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z