BBa_K1616024
type: ComponentDefinition
persistent identity: BBa K1616024
display id: BBa_K1616024
version: 1
was generated by: iGEM to SBOL conversion
description: VVD and YFP complex
created: 2015-09-17T11:00:00Z
modified: 2016-01-25T11:10:05Z
mutable provenance: Assembly of the 2 parts of VVD and split YFP
owned by: james
owned by: myers
mutable description: Vivid (VVD) is the smallest known Light???oxygen???voltage (LOV) domain protein and photo-inducible dimer. Isolated from Neurospora crassa, VVD forms a homodimer in response to a blue-light stimulus. Then, a split protein is a protein whose sequence has been divided into two (or more) different parts. The yellow-fluorescent (YFP) protein will only express fluorescence when its two parts will be reunited. The part is coding for the homodimer VVD links by an integration of specific sequence to the N terminal of the YFP split. The downstream part of this composite is double T7 terminator (BBa_B0015). So, this part works with BBa_K1616001. In absence of blue-light, the conformation of the VVD photoreceptor will prevent the formation of the complete fluorescent protein while in presence of the light signal the YFP protein will be reconstituted leading to the fast expression of a yellow fluorescence in our bacteria.
discontinued: false
dominant: false
experience: experience/None
group u list: _2033_
m user id: 4206
owner id: 22805
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The sequence of VVD had 2 illegal sites PstI; that have been removed. Also, the N terminal of YFP had 1 illegal site PstI
star: false
creator: Johanna Chesnel
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1616001
component: BBa_K1616002
sequence annotation: BBa_K1616001
sequence annotation: BBa_K1616002
sequence: https://synbiohub.org/public/igem/BBa_K1616024_sequence/1

BBa_K1616001
type: Component
persistent identity: component2472434
display id: component2472434
version: 1
top level: BBa_K1616024
definition: VVD-YC
access: public

BBa_K1616002
type: Component
persistent identity: component2472450
display id: component2472450
version: 1
top level: BBa_K1616024
definition: VVD-YN
access: public

BBa_K1616001
type: SequenceAnnotation
persistent identity: annotation2472434
display id: annotation2472434
version: 1
top level: BBa_K1616024
location: https://synbiohub.org/public/igem/BBa_K1616024/annotation2472434/range2472434/1
component: BBa_K1616001

https://synbiohub.org/public/igem/BBa_K1616024/annotation2472434/range2472434/1
type: Range
persistent identity: range2472434
display id: range2472434
version: 1
top level: BBa_K1616024
direction: direction/reverse
start: 1
end: 830
orientation: inline

BBa_K1616002
type: SequenceAnnotation
persistent identity: annotation2472450
display id: annotation2472450
version: 1
top level: BBa_K1616024
location: https://synbiohub.org/public/igem/BBa_K1616024/annotation2472450/range2472450/1
component: BBa_K1616002

https://synbiohub.org/public/igem/BBa_K1616024/annotation2472450/range2472450/1
type: Range
persistent identity: range2472450
display id: range2472450
version: 1
top level: BBa_K1616024
direction: direction/reverse
start: 839
end: 1928
orientation: inline

VVD-YC
type: ComponentDefinition
persistent identity: BBa K1616001
display id: BBa_K1616001
version: 1
was generated by: iGEM to SBOL conversion
description: VVD linked to YC155 (YFP Cter split) with promoter T7
created: 2015-09-16T11:00:00Z
modified: 2015-09-22T06:42:02Z
mutable provenance: This part have been created thank to gblock, our team have assembled the sequence of photoreceptor VVD (without illegal site), a linker(1) and then the C terminal of YFP split(1). (1) Tom Kerppola, Ph. D, investigator at the Howard Hughes Medical Institute as well as Professor in the University of Michigan
owned by: james
owned by: myers
mutable description: Vivid (VVD) is the smallest known Light???oxygen???voltage (LOV) domain protein and photo-inducible dimer. Isolated from Neurospora crassa, VVD forms a homo-dimer in response to a blue-light stimulus. Then, a split protein is a protein whose sequence has been divided into two (or more) different parts. The yellow-fluorescent (YFP) protein will only express fluorescence when its two parts will be reunited. The Part is coding for the homodimer VVD link by an integration of specific sequence to the C terminal of the YFP split. So, this part works with BBa_K1616002. In absence of blue-light, the conformation of the VVD photoreceptor will prevent the formation of the complete fluorescent protein while in presence of the light signal the YFP protein will be reconstituted leading to the fast expression of a yellow fluorescence in our bacteria.
discontinued: false
dominant: false
experience: experience/None
group u list: _2033_
m user id: 22805
owner id: 22805
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: The sequence of VVD had 2 illegal sites PstI; that have been removed.
star: false
creator: Johanna Chesnel
type: DnaRegion
role: SO:0000316
role: partType/Coding
component: BBa_I712074
component: BBa_K1616021
component: BBa_J61100
sequence annotation: BBa_I712074
sequence annotation: BBa_J61100
sequence annotation: BBa_K1616021
sequence: https://synbiohub.org/public/igem/BBa_K1616001_sequence/1

BBa_I712074
type: Component
persistent identity: component2471216
display id: component2471216
version: 1
top level: VVD-YC
definition: BBa_I712074
access: public

BBa_K1616021
type: Component
persistent identity: component2471224
display id: component2471224
version: 1
top level: VVD-YC
definition: VVD-YC
access: public

BBa_J61100
type: Component
persistent identity: component2471217
display id: component2471217
version: 1
top level: VVD-YC
definition: BBa_J61100
access: public

BBa_I712074
type: SequenceAnnotation
persistent identity: annotation2471216
display id: annotation2471216
version: 1
top level: VVD-YC
location: https://synbiohub.org/public/igem/BBa_K1616001/annotation2471216/range2471216/1
component: BBa_I712074

https://synbiohub.org/public/igem/BBa_K1616001/annotation2471216/range2471216/1
type: Range
persistent identity: range2471216
display id: range2471216
version: 1
top level: VVD-YC
direction: direction/reverse
start: 1
end: 46
orientation: inline

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2471217
display id: annotation2471217
version: 1
top level: VVD-YC
location: https://synbiohub.org/public/igem/BBa_K1616001/annotation2471217/range2471217/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K1616001/annotation2471217/range2471217/1
type: Range
persistent identity: range2471217
display id: range2471217
version: 1
top level: VVD-YC
direction: direction/reverse
start: 55
end: 66
orientation: inline

BBa_K1616021
type: SequenceAnnotation
persistent identity: annotation2471224
display id: annotation2471224
version: 1
top level: VVD-YC
location: https://synbiohub.org/public/igem/BBa_K1616001/annotation2471224/range2471224/1
component: BBa_K1616021

https://synbiohub.org/public/igem/BBa_K1616001/annotation2471224/range2471224/1
type: Range
persistent identity: range2471224
display id: range2471224
version: 1
top level: VVD-YC
direction: direction/reverse
start: 75
end: 830
orientation: inline

VVD-YN
type: ComponentDefinition
persistent identity: BBa K1616002
display id: BBa_K1616002
version: 1
was generated by: iGEM to SBOL conversion
description: VVD linked to YN155 (YFP Nter split) with double terminator T7
created: 2015-09-16T11:00:00Z
modified: 2015-09-22T06:42:24Z
mutable provenance: Assembly the sequence of photoreceptor VVD (without illegal site), a linker(1) and then the N terminal of YFP split(1)
owned by: james
owned by: myers
mutable description: Vivid (VVD) is the smallest known Light???oxygen???voltage (LOV) domain protein and photo-inducible dimer. Isolated from Neurospora crassa, VVD forms a homodimer in response to a blue-light stimulus. Then, a split protein is a protein whose sequence has been divided into two (or more) different parts. The yellow-fluorescent (YFP) protein will only express fluorescence when its two parts will be reunited. The part is coding for the homodimer VVD links by an integration of specific sequence to the N terminal of the YFP split. The downstream part of this composite is double T7 terminator (BBa_B0015). So, this part works with BBa_K1616001. In absence of blue-light, the conformation of the VVD photoreceptor will prevent the formation of the complete fluorescent protein while in presence of the light signal the YFP protein will be reconstituted leading to the fast expression of a yellow fluorescence in our bacteria.
discontinued: false
dominant: false
experience: experience/None
group u list: _2033_
m user id: 22805
owner id: 22805
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: (1) Tom Kerppola, Ph. D, investigator at the Howard Hughes Medical Institute as well as Professor in the University of Michigan Hu CD, Chinenov Y, Kerppola TK. Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation. Mol Cell. 2002;9(4):789???98.
star: false
creator: Johanna Chesnel
type: DnaRegion
role: SO:0000316
role: partType/Coding
component: BBa_B0015
component: BBa_K1616020
component: BBa_J61100
sequence annotation: BBa_J61100
sequence annotation: BBa_K1616020
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1616002_sequence/1

BBa_B0015
type: Component
persistent identity: component2471090
display id: component2471090
version: 1
top level: VVD-YN
definition: BBa_B0015
access: public

BBa_K1616020
type: Component
persistent identity: component2471083
display id: component2471083
version: 1
top level: VVD-YN
definition: VVD-YN
access: public

BBa_J61100
type: Component
persistent identity: component2471076
display id: component2471076
version: 1
top level: VVD-YN
definition: BBa_J61100
access: public

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2471076
display id: annotation2471076
version: 1
top level: VVD-YN
location: https://synbiohub.org/public/igem/BBa_K1616002/annotation2471076/range2471076/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K1616002/annotation2471076/range2471076/1
type: Range
persistent identity: range2471076
display id: range2471076
version: 1
top level: VVD-YN
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K1616020
type: SequenceAnnotation
persistent identity: annotation2471083
display id: annotation2471083
version: 1
top level: VVD-YN
location: https://synbiohub.org/public/igem/BBa_K1616002/annotation2471083/range2471083/1
component: BBa_K1616020

https://synbiohub.org/public/igem/BBa_K1616002/annotation2471083/range2471083/1
type: Range
persistent identity: range2471083
display id: range2471083
version: 1
top level: VVD-YN
direction: direction/reverse
start: 21
end: 953
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2471090
display id: annotation2471090
version: 1
top level: VVD-YN
location: https://synbiohub.org/public/igem/BBa_K1616002/annotation2471090/range2471090/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1616002/annotation2471090/range2471090/1
type: Range
persistent identity: range2471090
display id: range2471090
version: 1
top level: VVD-YN
direction: direction/reverse
start: 962
end: 1090
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_I712074
type: ComponentDefinition
persistent identity: BBa I712074
display id: BBa_I712074
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter (strong promoter from T7 bacteriophage)
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:07:46Z
mutable provenance: T7 bacteriophage
owned by: james
owned by: myers
mutable description: T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes.
discontinued: false
dominant: false
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1856
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Rok Gaber
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I712074_sequence/1

BBa_J61100
type: ComponentDefinition
persistent identity: BBa J61100
display id: BBa_J61100
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
created: 2007-01-28T12:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: {{JCA_Arkin_RBSFamily}}
discontinued: false
dominant: true
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61100_sequence/1

VVD-YC
type: ComponentDefinition
persistent identity: BBa K1616021
display id: BBa_K1616021
version: 1
was generated by: iGEM to SBOL conversion
description: VVD linked to YC155 (YFP Cter split)
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T05:28:45Z
mutable provenance: Assembly of 3 parts: BBa_K1616014, BBa_K1616015 and BBa_K1616016 without scar
owned by: james
owned by: myers
mutable description: See BBa_K1616001. This part have been created in order to have the three parts without scars (VVD,linker and YFP Nterminal).
discontinued: false
dominant: false
experience: experience/None
group u list: _2033_
m user id: 22805
owner id: 22805
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No separation between the 3 parts.
star: false
creator: Johanna Chesnel
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1616015
component: BBa_K1616014
component: BBa_K1616016
sequence annotation: BBa_K1616014
sequence annotation: BBa_K1616015
sequence annotation: BBa_K1616016
sequence: https://synbiohub.org/public/igem/BBa_K1616021_sequence/1

BBa_K1616015
type: Component
persistent identity: component2471213
display id: component2471213
version: 1
top level: VVD-YC
definition: Linker-FOS
access: public

BBa_K1616014
type: Component
persistent identity: component2471211
display id: component2471211
version: 1
top level: VVD-YC
definition: VVD
access: public

BBa_K1616016
type: Component
persistent identity: component2471215
display id: component2471215
version: 1
top level: VVD-YC
definition: YFP-C
access: public

BBa_K1616014
type: SequenceAnnotation
persistent identity: annotation2471211
display id: annotation2471211
version: 1
top level: VVD-YC
location: https://synbiohub.org/public/igem/BBa_K1616021/annotation2471211/range2471211/1
component: BBa_K1616014

https://synbiohub.org/public/igem/BBa_K1616021/annotation2471211/range2471211/1
type: Range
persistent identity: range2471211
display id: range2471211
version: 1
top level: VVD-YC
direction: direction/reverse
start: 1
end: 450
orientation: inline

BBa_K1616015
type: SequenceAnnotation
persistent identity: annotation2471213
display id: annotation2471213
version: 1
top level: VVD-YC
location: https://synbiohub.org/public/igem/BBa_K1616021/annotation2471213/range2471213/1
component: BBa_K1616015

https://synbiohub.org/public/igem/BBa_K1616021/annotation2471213/range2471213/1
type: Range
persistent identity: range2471213
display id: range2471213
version: 1
top level: VVD-YC
direction: direction/reverse
start: 451
end: 501
orientation: inline

BBa_K1616016
type: SequenceAnnotation
persistent identity: annotation2471215
display id: annotation2471215
version: 1
top level: VVD-YC
location: https://synbiohub.org/public/igem/BBa_K1616021/annotation2471215/range2471215/1
component: BBa_K1616016

https://synbiohub.org/public/igem/BBa_K1616021/annotation2471215/range2471215/1
type: Range
persistent identity: range2471215
display id: range2471215
version: 1
top level: VVD-YC
direction: direction/reverse
start: 502
end: 756
orientation: inline

VVD-YN
type: ComponentDefinition
persistent identity: BBa K1616020
display id: BBa_K1616020
version: 1
was generated by: iGEM to SBOL conversion
description: VVD linked to YN155 (YFP Nter split)
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T04:55:03Z
mutable provenance: Assembly of 3 parts: BBa_K1616014, BBa_K1616018 and BBa_K1616017 without scars
owned by: james
owned by: myers
mutable description: See BBa_K1616002. This part have been created in order to have the three parts without scars (VVD,linker and YFP Nterminal).
discontinued: false
dominant: false
experience: experience/None
group u list: _2033_
m user id: 22805
owner id: 22805
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No separation between the 3 parts.
star: false
creator: Johanna Chesnel
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1616017
component: BBa_K1616018
component: BBa_K1616014
sequence annotation: BBa_K1616014
sequence annotation: BBa_K1616018
sequence annotation: BBa_K1616017
sequence: https://synbiohub.org/public/igem/BBa_K1616020_sequence/1

BBa_K1616017
type: Component
persistent identity: component2471075
display id: component2471075
version: 1
top level: VVD-YN
definition: YFP-N
access: public

BBa_K1616018
type: Component
persistent identity: component2471073
display id: component2471073
version: 1
top level: VVD-YN
definition: Linker-JUN
access: public

BBa_K1616014
type: Component
persistent identity: component2471071
display id: component2471071
version: 1
top level: VVD-YN
definition: VVD
access: public

BBa_K1616014
type: SequenceAnnotation
persistent identity: annotation2471071
display id: annotation2471071
version: 1
top level: VVD-YN
location: https://synbiohub.org/public/igem/BBa_K1616020/annotation2471071/range2471071/1
component: BBa_K1616014

https://synbiohub.org/public/igem/BBa_K1616020/annotation2471071/range2471071/1
type: Range
persistent identity: range2471071
display id: range2471071
version: 1
top level: VVD-YN
direction: direction/reverse
start: 1
end: 450
orientation: inline

BBa_K1616018
type: SequenceAnnotation
persistent identity: annotation2471073
display id: annotation2471073
version: 1
top level: VVD-YN
location: https://synbiohub.org/public/igem/BBa_K1616020/annotation2471073/range2471073/1
component: BBa_K1616018

https://synbiohub.org/public/igem/BBa_K1616020/annotation2471073/range2471073/1
type: Range
persistent identity: range2471073
display id: range2471073
version: 1
top level: VVD-YN
direction: direction/reverse
start: 451
end: 465
orientation: inline

BBa_K1616017
type: SequenceAnnotation
persistent identity: annotation2471075
display id: annotation2471075
version: 1
top level: VVD-YN
location: https://synbiohub.org/public/igem/BBa_K1616020/annotation2471075/range2471075/1
component: BBa_K1616017

https://synbiohub.org/public/igem/BBa_K1616020/annotation2471075/range2471075/1
type: Range
persistent identity: range2471075
display id: range2471075
version: 1
top level: VVD-YN
direction: direction/reverse
start: 466
end: 933
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

VVD
type: ComponentDefinition
persistent identity: BBa K1616014
display id: BBa_K1616014
version: 1
was generated by: iGEM to SBOL conversion
description: VVD - homodimer photoreceptor
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T02:54:35Z
mutable provenance: VVD was isolated from Neurospora crassa.
owned by: james
owned by: myers
mutable description: Among other photoreceptors, Vivid (VVD) is the smallest known Light???oxygen???voltage (LOV) domain protein and photo-inducible dimer. Isolated from Neurospora crassa, VVD forms a homo-dimer in response to a blue-light stimulus. The LOV domain, present in VVD, is a small blue-light sensing domain found in prokaryotes, fungi and plants. After blue-light activation, a covalent bond is formed between the co-factor Flavin mononucleotide (FMN) and one of the cysteine residue. This bond leads to a conformational change inducing functions by dissociating the C-terminal a-helix (Ja) and the LOV-core. In VVD, this undock triggers homodimerization (Bilwes, Dunlap, & Crane, 2007; M??ller & Weber, 2013). Contrary to other photoreceptors, VVD is a small protein with 150 amino-acids facilitating accurate molecular design and avoiding steric issues. Moreover, it is a homo-dimer when most of photo-inducible dimers are heterodimers. In addition, the use of VVD is easy; and doesn???t need any addition of co-factors: VVD works with Flavin adenine dinucleotide (FAD) which is already abundant in eukaryote and prokaryote cells (M??ller & Weber, 2013; Nihongaki, Suzuki, Kawano, & Sato, 2014).
discontinued: false
dominant: false
experience: experience/None
group u list: _2033_
m user id: 22805
owner id: 22805
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We found illegal sites (PstI) into VVD sequence, those one have been removed.
star: false
creator: Johanna Chesnel
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: VVD
sequence: https://synbiohub.org/public/igem/BBa_K1616014_sequence/1

VVD
type: SequenceAnnotation
persistent identity: annotation2470046
display id: annotation2470046
version: 1
top level: VVD
location: https://synbiohub.org/public/igem/BBa_K1616014/annotation2470046/range2470046/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1616014/annotation2470046/range2470046/1
type: Range
persistent identity: range2470046
display id: range2470046
version: 1
top level: VVD
direction: direction/forward
start: 1
end: 450
orientation: inline

Linker-FOS
type: ComponentDefinition
persistent identity: BBa K1616015
display id: BBa_K1616015
version: 1
was generated by: iGEM to SBOL conversion
description: Linker FOS of 17 aa
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T03:09:19Z
mutable provenance: Linker used in association with VVD (BBa_K1616014)
owned by: james
owned by: myers
mutable description: This part is a sequence coding for 17 aa useful to link two proteins
discontinued: false
dominant: false
experience: experience/None
group u list: _2033_
m user id: 22805
owner id: 22805
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Illegal sites have been checked
star: false
creator: Johanna Chesnel
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: linker
sequence: https://synbiohub.org/public/igem/BBa_K1616015_sequence/1

linker
type: SequenceAnnotation
persistent identity: annotation2470111
display id: annotation2470111
version: 1
top level: Linker-FOS
location: https://synbiohub.org/public/igem/BBa_K1616015/annotation2470111/range2470111/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1616015/annotation2470111/range2470111/1
type: Range
persistent identity: range2470111
display id: range2470111
version: 1
top level: Linker-FOS
direction: direction/forward
start: 1
end: 51
orientation: inline

YFP-C
type: ComponentDefinition
persistent identity: BBa K1616016
display id: BBa_K1616016
version: 1
was generated by: iGEM to SBOL conversion
description: YC155 - C terminal YFP split
created: 2015-09-17T11:00:00Z
modified: 2016-01-21T02:25:51Z
mutable provenance: This part works with BBa_K16160017.
owned by: james
owned by: myers
mutable description: A split protein is a protein whose sequence has been divided into two (or more) different parts. Often used to study protein-protein interactions, the protein can not perform its function until the parts are put back together. For instance, YFP, the yellow-fluorescent protein, will only express fluorescence when its two parts will be reunited. In normal condition, the production of a protein in response to a stimulus can easily reach several hours due to the many steps required for the protein synthesis. By using split-proteins, we are taking advantage of the absence of fluorescence when the two parts are apart. Indeed, the two parts of our split-YFP, when remaining separated, can be produced without being effective. Therefore, the overall process is far less time-consuming. However, to implement a light control on the fluorescence activation, a genetic construction gathering the VVD photoreceptor and our split-YFP has to be engineered. The new alternative approach for the visualization of protein interactiosn has been developed; the biomolecular fluorescence complementation (BiFC) techniques based on the complementation between fragments of fluorescent proteins; fragments of the yellow fluorescent protein (YFP) brought together by the association of two interaction partners fused to the fragments. They noticed that the spectral characteristics of BiFC of YFP were virtually identical to those of intact YFP.(Chang-Deng Hu, 2003)
discontinued: false
dominant: false
experience: experience/None
group u list: _2033_
m user id: 4206
owner id: 22805
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Illegal sites have been checked.
star: false
creator: Johanna Chesnel
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: YC155
sequence: https://synbiohub.org/public/igem/BBa_K1616016_sequence/1

YC155
type: SequenceAnnotation
persistent identity: annotation2470135
display id: annotation2470135
version: 1
top level: YFP-C
location: https://synbiohub.org/public/igem/BBa_K1616016/annotation2470135/range2470135/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1616016/annotation2470135/range2470135/1
type: Range
persistent identity: range2470135
display id: range2470135
version: 1
top level: YFP-C
direction: direction/forward
start: 1
end: 255
orientation: inline

YFP-N
type: ComponentDefinition
persistent identity: BBa K1616017
display id: BBa_K1616017
version: 1
was generated by: iGEM to SBOL conversion
description: YN155 - N terminal YFP split
created: 2015-09-17T11:00:00Z
modified: 2016-01-21T02:25:38Z
mutable provenance: This part works with BBa_K16160016
owned by: james
owned by: myers
mutable description: A split protein is a protein whose sequence has been divided into two (or more) different parts. Often used to study protein-protein interactions, the protein can not perform its function until the parts are put back together. For instance, YFP, the yellow-fluorescent protein, will only express fluorescence when its two parts will be reunited. In normal condition, the production of a protein in response to a stimulus can easily reach several hours due to the many steps required for the protein synthesis. By using split-proteins, we are taking advantage of the absence of fluorescence when the two parts are apart. Indeed, the two parts of our split-YFP, when remaining separated, can be produced without being effective. Therefore, the overall process is far less time-consuming. However, to implement a light control on the fluorescence activation, a genetic construction gathering the VVD photoreceptor and our split-YFP has to be engineered. The new alternative approach for the visualization of protein interactiosn has been developed; the biomolecular fluorescence complementation (BiFC) techniques based on the complementation between fragments of fluorescent proteins; fragments of the yellow fluorescent protein (YFP) brought together by the association of two interaction partners fused to the fragments. They noticed that the spectral characteristics of BiFC of YFP were virtually identical to those of intact YFP.(Chang-Deng Hu, 2003)
discontinued: false
dominant: false
experience: experience/None
group u list: _2033_
m user id: 4206
owner id: 22805
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Illegal sites have been checked.
star: false
creator: Johanna Chesnel
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: YN155
sequence: https://synbiohub.org/public/igem/BBa_K1616017_sequence/1

YN155
type: SequenceAnnotation
persistent identity: annotation2470148
display id: annotation2470148
version: 1
top level: YFP-N
location: https://synbiohub.org/public/igem/BBa_K1616017/annotation2470148/range2470148/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1616017/annotation2470148/range2470148/1
type: Range
persistent identity: range2470148
display id: range2470148
version: 1
top level: YFP-N
direction: direction/forward
start: 1
end: 468
orientation: inline

Linker-JUN
type: ComponentDefinition
persistent identity: BBa K1616018
display id: BBa_K1616018
version: 1
was generated by: iGEM to SBOL conversion
description: Linker JUN of 5 aa
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T03:37:41Z
mutable provenance: Linker used in association with YFP Nterminal split and VVD.
owned by: james
owned by: myers
mutable description: This part is a sequence coding for 5 aa useful to link VVD and YFP N terminal split protein.
discontinued: false
dominant: false
experience: experience/None
group u list: _2033_
m user id: 22805
owner id: 22805
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Illegal sites have been checked
star: false
creator: Johanna Chesnel
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: linker JUN
sequence: https://synbiohub.org/public/igem/BBa_K1616018_sequence/1

linker JUN
type: SequenceAnnotation
persistent identity: annotation2470177
display id: annotation2470177
version: 1
top level: Linker-JUN
location: https://synbiohub.org/public/igem/BBa_K1616018/annotation2470177/range2470177/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1616018/annotation2470177/range2470177/1
type: Range
persistent identity: range2470177
display id: range2470177
version: 1
top level: Linker-JUN
direction: direction/forward
start: 1
end: 15
orientation: inline

https://synbiohub.org/public/igem/BBa_K1616024_sequence/1
type: Sequence
persistent identity: BBa K1616024 sequence
display id: BBa_K1616024_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaatacaagctacttgttctttttgcatactagagaaagaggggacatactagagcacaccctgtatgcccctggcggctacgacatcatgggctacctgatccagatcatgaagcggcccaacccccaggtggaactgggccctgtggatacctctgtggccctgatcctgtgcgacctgaagcagaaagacacccccatcgtgtacgcctccgaggccttcctgtacatgaccggctactccaacgccgaggtgctgggccggaactgtagattcctccagtcccctgacggcatggtcaagcctaagtccacccggaaatacgtggactctaacaccatcaacaccatgcggaaggccatcgaccggaacgctgaggtgcaggtggaagtcgtgaacttcaagaagaacggccagcgcttcgtgaatttcctgaccatgatccccgtgcgggacgagacaggcgagtacagatactccatgggcttccagtgcgagacagagcgtccggcgtgcaaaatcccgaacgacctgaaacagaaagtcatgaaccacgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaatactagagaaagaggggacatactagagcacaccctgtatgcccctggcggctacgacatcatgggctacctgatccagatcatgaagcggcccaacccccaggtggaactgggccctgtggatacctctgtggccctgatcctgtgcgacctgaagcagaaagacacccccatcgtgtacgcctccgaggccttcctgtacatgaccggctactccaacgccgaggtgctgggccggaactgtagattcctccagtcccctgacggcatggtcaagcctaagtccacccggaaatacgtggactctaacaccatcaacaccatgcggaaggccatcgaccggaacgctgaggtgcaggtggaagtcgtgaacttcaagaagaacggccagcgcttcgtgaatttcctgaccatgatccccgtgcgggacgagacaggcgagtacagatactccatgggcttccagtgcgagacagagagatccatcgccaccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggccttcaatgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggcctagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1616001_sequence/1
type: Sequence
persistent identity: BBa K1616001 sequence
display id: BBa_K1616001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaatacaagctacttgttctttttgcatactagagaaagaggggacatactagagcacaccctgtatgcccctggcggctacgacatcatgggctacctgatccagatcatgaagcggcccaacccccaggtggaactgggccctgtggatacctctgtggccctgatcctgtgcgacctgaagcagaaagacacccccatcgtgtacgcctccgaggccttcctgtacatgaccggctactccaacgccgaggtgctgggccggaactgtagattcctccagtcccctgacggcatggtcaagcctaagtccacccggaaatacgtggactctaacaccatcaacaccatgcggaaggccatcgaccggaacgctgaggtgcaggtggaagtcgtgaacttcaagaagaacggccagcgcttcgtgaatttcctgaccatgatccccgtgcgggacgagacaggcgagtacagatactccatgggcttccagtgcgagacagagcgtccggcgtgcaaaatcccgaacgacctgaaacagaaagtcatgaaccacgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1616002_sequence/1
type: Sequence
persistent identity: BBa K1616002 sequence
display id: BBa_K1616002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggggacatactagagcacaccctgtatgcccctggcggctacgacatcatgggctacctgatccagatcatgaagcggcccaacccccaggtggaactgggccctgtggatacctctgtggccctgatcctgtgcgacctgaagcagaaagacacccccatcgtgtacgcctccgaggccttcctgtacatgaccggctactccaacgccgaggtgctgggccggaactgtagattcctccagtcccctgacggcatggtcaagcctaagtccacccggaaatacgtggactctaacaccatcaacaccatgcggaaggccatcgaccggaacgctgaggtgcaggtggaagtcgtgaacttcaagaagaacggccagcgcttcgtgaatttcctgaccatgatccccgtgcgggacgagacaggcgagtacagatactccatgggcttccagtgcgagacagagagatccatcgccaccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggccttcaatgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggcctagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I712074_sequence/1
type: Sequence
persistent identity: BBa I712074 sequence
display id: BBa_I712074_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaatacaagctacttgttctttttgca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61100_sequence/1
type: Sequence
persistent identity: BBa J61100 sequence
display id: BBa_J61100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggggaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1616021_sequence/1
type: Sequence
persistent identity: BBa K1616021 sequence
display id: BBa_K1616021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cacaccctgtatgcccctggcggctacgacatcatgggctacctgatccagatcatgaagcggcccaacccccaggtggaactgggccctgtggatacctctgtggccctgatcctgtgcgacctgaagcagaaagacacccccatcgtgtacgcctccgaggccttcctgtacatgaccggctactccaacgccgaggtgctgggccggaactgtagattcctccagtcccctgacggcatggtcaagcctaagtccacccggaaatacgtggactctaacaccatcaacaccatgcggaaggccatcgaccggaacgctgaggtgcaggtggaagtcgtgaacttcaagaagaacggccagcgcttcgtgaatttcctgaccatgatccccgtgcgggacgagacaggcgagtacagatactccatgggcttccagtgcgagacagagcgtccggcgtgcaaaatcccgaacgacctgaaacagaaagtcatgaaccacgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1616020_sequence/1
type: Sequence
persistent identity: BBa K1616020 sequence
display id: BBa_K1616020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cacaccctgtatgcccctggcggctacgacatcatgggctacctgatccagatcatgaagcggcccaacccccaggtggaactgggccctgtggatacctctgtggccctgatcctgtgcgacctgaagcagaaagacacccccatcgtgtacgcctccgaggccttcctgtacatgaccggctactccaacgccgaggtgctgggccggaactgtagattcctccagtcccctgacggcatggtcaagcctaagtccacccggaaatacgtggactctaacaccatcaacaccatgcggaaggccatcgaccggaacgctgaggtgcaggtggaagtcgtgaacttcaagaagaacggccagcgcttcgtgaatttcctgaccatgatccccgtgcgggacgagacaggcgagtacagatactccatgggcttccagtgcgagacagagagatccatcgccaccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggccttcaatgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggcctag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1616014_sequence/1
type: Sequence
persistent identity: BBa K1616014 sequence
display id: BBa_K1616014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cacaccctgtatgcccctggcggctacgacatcatgggctacctgatccagatcatgaagcggcccaacccccaggtggaactgggccctgtggatacctctgtggccctgatcctgtgcgacctgaagcagaaagacacccccatcgtgtacgcctccgaggccttcctgtacatgaccggctactccaacgccgaggtgctgggccggaactgtagattcctccagtcccctgacggcatggtcaagcctaagtccacccggaaatacgtggactctaacaccatcaacaccatgcggaaggccatcgaccggaacgctgaggtgcaggtggaagtcgtgaacttcaagaagaacggccagcgcttcgtgaatttcctgaccatgatccccgtgcgggacgagacaggcgagtacagatactccatgggcttccagtgcgagacagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1616015_sequence/1
type: Sequence
persistent identity: BBa K1616015 sequence
display id: BBa_K1616015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgtccggcgtgcaaaatcccgaacgacctgaaacagaaagtcatgaaccac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1616016_sequence/1
type: Sequence
persistent identity: BBa K1616016 sequence
display id: BBa_K1616016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1616017_sequence/1
type: Sequence
persistent identity: BBa K1616017 sequence
display id: BBa_K1616017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggccttcaatgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggcctag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1616018_sequence/1
type: Sequence
persistent identity: BBa K1616018 sequence
display id: BBa_K1616018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agatccatcgccacc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z