##gff-version 3
##sequence-region BBa_K1617000 1 1290
BBa_K1617000	.	start_codon	1	3	.	+	0	ID=annotation2457065;Name=Start ATG
BBa_K1617000	.	engineered_region	577	659	.	+	0	ID=annotation2457075;Name=BBa_K1617010
BBa_K1617000	.	point_mutation	551	551	.	+	0	ID=annotation2457251;Name=Delete BamHI site
BBa_K1617000	.	point_mutation	662	662	.	+	0	ID=annotation2457280;Name=s_mutation
BBa_K1617000	.	point_mutation	1016	1016	.	+	0	ID=annotation2457283;Name=Delete Spe1 site
BBa_K1617000	.	point_mutation	1290	1290	.	+	0	ID=annotation2457286;Name=Delete Pst1 site
BBa_K1617000	.	sequence_feature	430	432	.	+	0	ID=annotation2457073;Name=M144A
BBa_K1617000	.	sequence_feature	577	659	.	+	0	ID=annotation2457215;Name=MCS
BBa_K1617000	.	sequence_feature	1203	1205	.	+	0	ID=annotation2457232;Name=M401G
BBa_K1617000	.	point_mutation	305	305	.	+	0	ID=annotation2457241;Name=Delete Pst1 site
BBa_K1617000	.	point_mutation	713	713	.	+	0	ID=annotation2457282;Name=Delete Pst1 site
BBa_K1617000	.	point_mutation	227	227	.	+	0	ID=annotation2457231;Name=Delete Xba1 site
>BBa_K1617000
atggcacaagtcattaataccaacagcctctcgctgatcactcaaaataatatcaacaagaaccagtctgcgctgtcga
gttctatcgagcgtctgtcttctggcttgcgtattaacagcgcgaaggatgacgcagcgggtcaggcgattgctaaccg
tttcacctctaacattaaaggcctgactcaggcggcccgtaacgccaacgacggtatctccgttgcccagaccaccgaa
ggcgcgctgtccgaaatcaacaacaacttacagcgtgtgcgtgaactgacggtacaggccactaccggcactaactctg
agtctgatctgtcttctatccaggacgaaattaaatcccgtctggatgaaattgaccgcgtatctggtcagacccagtt
caacggcgtgaacgtgctggcaaaaaatggctccgcaaaaatccaggttggcgcaaatgataaccagactatcactatc
gatctgaagcagattgatgctaaaactcttggccttgatggttttagcgttaaaaataacgatacagttaccaccagtg
ctccagtaactgcttttggtgctggatccgccggcgtcgacatgggtggcagcatgggtggcagcatgaagcttagatc
taccggttcaggcggtacacctgttcagattgataataccgcaggttccgcaactgccaaccttggtgctgttagctta
gtaaaactgcaagattccaagggtaatgataccgatacatatgcgcttaaagatacaaatggcaatctttacgctgcgg
atgtgaatgaaactactggtgctgtttctgttaaaactattacctatactgactcttccggtgccgccagttctccaac
cgcggtcaaactgggcggagatgatggcaaaacagaagtggtcgatattgatggtaaaacatacgattctgccgattta
aatggcggtaatctgcaaacaggtttgactgctggtggtgaggctctgactgctgttgcaaatggtaaaaccacggacc
cgctgaaagcgctggacgatgctatcgcatctgtagacaaattccgttcttccctcggtgcggtgcaaaaccgtctgga
ttccgcggttaccaacctgaacaacaccactaccaacctgtctgaagcgcagtcccgtattcaggacgccgactatgcg
accgaagtgtccaatggttcgaaagcgcagatcatccagcaggccggtaactccgtgttggcaaaagctaaccaggtac
cgcagcaggttctgtctctgctgcaaggttaa