##gff-version 3
##sequence-region BBa_K1617001 1 1314
BBa_K1617001	.	CDS	19	1314	.	+	0	ID=BBa_K1617000;Name=BBa_K1617000
BBa_K1617001	.	point_mutation	680	680	.	+	0	ID=annotation2457280;Name=s_mutation;Parent=BBa_K1617000
BBa_K1617001	.	point_mutation	1034	1034	.	+	0	ID=annotation2457283;Name=Delete Spe1 site;Parent=BBa_K1617000
BBa_K1617001	.	point_mutation	323	323	.	+	0	ID=annotation2457241;Name=Delete Pst1 site;Parent=BBa_K1617000
BBa_K1617001	.	sequence_feature	448	450	.	+	0	ID=annotation2457073;Name=M144A;Parent=BBa_K1617000
BBa_K1617001	.	sequence_feature	595	677	.	+	0	ID=annotation2457215;Name=MCS;Parent=BBa_K1617000
BBa_K1617001	.	point_mutation	245	245	.	+	0	ID=annotation2457231;Name=Delete Xba1 site;Parent=BBa_K1617000
BBa_K1617001	.	point_mutation	731	731	.	+	0	ID=annotation2457282;Name=Delete Pst1 site;Parent=BBa_K1617000
BBa_K1617001	.	point_mutation	569	569	.	+	0	ID=annotation2457251;Name=Delete BamHI site;Parent=BBa_K1617000
BBa_K1617001	.	start_codon	19	21	.	+	0	ID=annotation2457065;Name=Start ATG;Parent=BBa_K1617000
BBa_K1617001	.	engineered_region	595	677	.	+	0	ID=annotation2457075;Name=BBa_K1617010;Parent=BBa_K1617000
BBa_K1617001	.	sequence_feature	1221	1223	.	+	0	ID=annotation2457232;Name=M401G;Parent=BBa_K1617000
BBa_K1617001	.	point_mutation	1308	1308	.	+	0	ID=annotation2457286;Name=Delete Pst1 site;Parent=BBa_K1617000
BBa_K1617001	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1617001	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K1617001
aaagaggagaaatactagatggcacaagtcattaataccaacagcctctcgctgatcactcaaaataatatcaacaaga
accagtctgcgctgtcgagttctatcgagcgtctgtcttctggcttgcgtattaacagcgcgaaggatgacgcagcggg
tcaggcgattgctaaccgtttcacctctaacattaaaggcctgactcaggcggcccgtaacgccaacgacggtatctcc
gttgcccagaccaccgaaggcgcgctgtccgaaatcaacaacaacttacagcgtgtgcgtgaactgacggtacaggcca
ctaccggcactaactctgagtctgatctgtcttctatccaggacgaaattaaatcccgtctggatgaaattgaccgcgt
atctggtcagacccagttcaacggcgtgaacgtgctggcaaaaaatggctccgcaaaaatccaggttggcgcaaatgat
aaccagactatcactatcgatctgaagcagattgatgctaaaactcttggccttgatggttttagcgttaaaaataacg
atacagttaccaccagtgctccagtaactgcttttggtgctggatccgccggcgtcgacatgggtggcagcatgggtgg
cagcatgaagcttagatctaccggttcaggcggtacacctgttcagattgataataccgcaggttccgcaactgccaac
cttggtgctgttagcttagtaaaactgcaagattccaagggtaatgataccgatacatatgcgcttaaagatacaaatg
gcaatctttacgctgcggatgtgaatgaaactactggtgctgtttctgttaaaactattacctatactgactcttccgg
tgccgccagttctccaaccgcggtcaaactgggcggagatgatggcaaaacagaagtggtcgatattgatggtaaaaca
tacgattctgccgatttaaatggcggtaatctgcaaacaggtttgactgctggtggtgaggctctgactgctgttgcaa
atggtaaaaccacggacccgctgaaagcgctggacgatgctatcgcatctgtagacaaattccgttcttccctcggtgc
ggtgcaaaaccgtctggattccgcggttaccaacctgaacaacaccactaccaacctgtctgaagcgcagtcccgtatt
caggacgccgactatgcgaccgaagtgtccaatggttcgaaagcgcagatcatccagcaggccggtaactccgtgttgg
caaaagctaaccaggtaccgcagcaggttctgtctctgctgcaaggttaa