LOCUS BBa_K1617005 1357 bp DNA linear 28-Dec-2024 DEFINITION Prom110_FliC D3 3xMet ACCESSION BBa_K1617005 VERSION BBa_K1617005.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23110 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 62..64 /label=Start ATG misc_feature 491..493 /label=M144A misc_feature 638..720 /label=BBa_K1617010 misc_feature 638..720 /label=MCS misc_feature 288..288 /label=Delete Xba1 site misc_feature 1264..1266 /label=M401G misc_feature 366..366 /label=Delete Pst1 site misc_feature 612..612 /label=Delete BamHI site misc_feature 723..723 /label=s_mutation misc_feature 774..774 /label=Delete Pst1 site misc_feature 1077..1077 /label=Delete Spe1 site misc_feature 1351..1351 /label=Delete Pst1 site CDS 62..1357 /label=BBa_K1617000 ORIGIN 1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaatacta 61 gatggcacaa gtcattaata ccaacagcct ctcgctgatc actcaaaata atatcaacaa 121 gaaccagtct gcgctgtcga gttctatcga gcgtctgtct tctggcttgc gtattaacag 181 cgcgaaggat gacgcagcgg gtcaggcgat tgctaaccgt ttcacctcta acattaaagg 241 cctgactcag gcggcccgta acgccaacga cggtatctcc gttgcccaga ccaccgaagg 301 cgcgctgtcc gaaatcaaca acaacttaca gcgtgtgcgt gaactgacgg tacaggccac 361 taccggcact aactctgagt ctgatctgtc ttctatccag gacgaaatta aatcccgtct 421 ggatgaaatt gaccgcgtat ctggtcagac ccagttcaac ggcgtgaacg tgctggcaaa 481 aaatggctcc gcaaaaatcc aggttggcgc aaatgataac cagactatca ctatcgatct 541 gaagcagatt gatgctaaaa ctcttggcct tgatggtttt agcgttaaaa ataacgatac 601 agttaccacc agtgctccag taactgcttt tggtgctgga tccgccggcg tcgacatggg 661 tggcagcatg ggtggcagca tgaagcttag atctaccggt tcaggcggta cacctgttca 721 gattgataat accgcaggtt ccgcaactgc caaccttggt gctgttagct tagtaaaact 781 gcaagattcc aagggtaatg ataccgatac atatgcgctt aaagatacaa atggcaatct 841 ttacgctgcg gatgtgaatg aaactactgg tgctgtttct gttaaaacta ttacctatac 901 tgactcttcc ggtgccgcca gttctccaac cgcggtcaaa ctgggcggag atgatggcaa 961 aacagaagtg gtcgatattg atggtaaaac atacgattct gccgatttaa atggcggtaa 1021 tctgcaaaca ggtttgactg ctggtggtga ggctctgact gctgttgcaa atggtaaaac 1081 cacggacccg ctgaaagcgc tggacgatgc tatcgcatct gtagacaaat tccgttcttc 1141 cctcggtgcg gtgcaaaacc gtctggattc cgcggttacc aacctgaaca acaccactac 1201 caacctgtct gaagcgcagt cccgtattca ggacgccgac tatgcgaccg aagtgtccaa 1261 tggttcgaaa gcgcagatca tccagcaggc cggtaactcc gtgttggcaa aagctaacca 1321 ggtaccgcag caggttctgt ctctgctgca aggttaa //