BBa_K1617005 1 BBa_K1617005 Prom110_FliC D3 3xMet 2015-09-17T11:00:00Z 2015-09-18T09:13:52Z sss sss false false _2034_ 19714 19714 9 false ss false Johann Bauerfeind component2472595 1 BBa_J23110 component2472610 1 BBa_K1617000 component2472597 1 BBa_B0034 annotation2472610 1 BBa_K1617000 range2472610 1 62 1357 annotation2472595 1 BBa_J23110 range2472595 1 1 35 annotation2472597 1 BBa_B0034 range2472597 1 44 55 BBa_J23110 1 BBa_J23110 constitutive promoter family member 2006-08-16T11:00:00Z 2015-08-31T04:08:40Z Later Later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K1617000 1 fliC_MCS Modular Flagellin - FliC_MCS 2015-09-14T11:00:00Z 2015-09-23T06:07:18Z Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655) Gene ID: 949101 Synonyms: ECK1922; flaF; H; hag; JW1908 = Contructing a multimodular Flagellin = == 1 Theory == === 1.1 Aim === Design of a functional flaggelin variant which does not contain the variable d3 flagellin domain anymore but instead carries a polylinker region (MCS). This polylinker region is the product of an inserted multi cloning side in place of the endogeneous d3 coding sequence. false false _2034_ 19714 19714 9 true = 1. Modularity of D3 Domain = = 2. Substitution of Methionins = = 3. Removing restriction sites = = 4. Compatibility of Multi-Cloning-Site = false Johann Bauerfeind annotation2457075 1 BBa_K1617010 range2457075 1 577 659 annotation2457283 1 Delete Spe1 site range2457283 1 1016 1016 annotation2457282 1 Delete Pst1 site range2457282 1 713 713 annotation2457073 1 M144A range2457073 1 430 432 annotation2457215 1 MCS range2457215 1 577 659 annotation2457280 1 s_mutation range2457280 1 662 662 annotation2457231 1 Delete Xba1 site range2457231 1 227 227 annotation2457251 1 Delete BamHI site range2457251 1 551 551 annotation2457232 1 M401G range2457232 1 1203 1205 annotation2457241 1 Delete Pst1 site range2457241 1 305 305 annotation2457065 1 Start ATG range2457065 1 1 3 annotation2457286 1 Delete Pst1 site range2457286 1 1290 1290 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0034_sequence 1 aaagaggagaaa BBa_K1617000_sequence 1 atggcacaagtcattaataccaacagcctctcgctgatcactcaaaataatatcaacaagaaccagtctgcgctgtcgagttctatcgagcgtctgtcttctggcttgcgtattaacagcgcgaaggatgacgcagcgggtcaggcgattgctaaccgtttcacctctaacattaaaggcctgactcaggcggcccgtaacgccaacgacggtatctccgttgcccagaccaccgaaggcgcgctgtccgaaatcaacaacaacttacagcgtgtgcgtgaactgacggtacaggccactaccggcactaactctgagtctgatctgtcttctatccaggacgaaattaaatcccgtctggatgaaattgaccgcgtatctggtcagacccagttcaacggcgtgaacgtgctggcaaaaaatggctccgcaaaaatccaggttggcgcaaatgataaccagactatcactatcgatctgaagcagattgatgctaaaactcttggccttgatggttttagcgttaaaaataacgatacagttaccaccagtgctccagtaactgcttttggtgctggatccgccggcgtcgacatgggtggcagcatgggtggcagcatgaagcttagatctaccggttcaggcggtacacctgttcagattgataataccgcaggttccgcaactgccaaccttggtgctgttagcttagtaaaactgcaagattccaagggtaatgataccgatacatatgcgcttaaagatacaaatggcaatctttacgctgcggatgtgaatgaaactactggtgctgtttctgttaaaactattacctatactgactcttccggtgccgccagttctccaaccgcggtcaaactgggcggagatgatggcaaaacagaagtggtcgatattgatggtaaaacatacgattctgccgatttaaatggcggtaatctgcaaacaggtttgactgctggtggtgaggctctgactgctgttgcaaatggtaaaaccacggacccgctgaaagcgctggacgatgctatcgcatctgtagacaaattccgttcttccctcggtgcggtgcaaaaccgtctggattccgcggttaccaacctgaacaacaccactaccaacctgtctgaagcgcagtcccgtattcaggacgccgactatgcgaccgaagtgtccaatggttcgaaagcgcagatcatccagcaggccggtaactccgtgttggcaaaagctaaccaggtaccgcagcaggttctgtctctgctgcaaggttaa BBa_J23110_sequence 1 tttacggctagctcagtcctaggtacaatgctagc BBa_K1617005_sequence 1 tttacggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatggcacaagtcattaataccaacagcctctcgctgatcactcaaaataatatcaacaagaaccagtctgcgctgtcgagttctatcgagcgtctgtcttctggcttgcgtattaacagcgcgaaggatgacgcagcgggtcaggcgattgctaaccgtttcacctctaacattaaaggcctgactcaggcggcccgtaacgccaacgacggtatctccgttgcccagaccaccgaaggcgcgctgtccgaaatcaacaacaacttacagcgtgtgcgtgaactgacggtacaggccactaccggcactaactctgagtctgatctgtcttctatccaggacgaaattaaatcccgtctggatgaaattgaccgcgtatctggtcagacccagttcaacggcgtgaacgtgctggcaaaaaatggctccgcaaaaatccaggttggcgcaaatgataaccagactatcactatcgatctgaagcagattgatgctaaaactcttggccttgatggttttagcgttaaaaataacgatacagttaccaccagtgctccagtaactgcttttggtgctggatccgccggcgtcgacatgggtggcagcatgggtggcagcatgaagcttagatctaccggttcaggcggtacacctgttcagattgataataccgcaggttccgcaactgccaaccttggtgctgttagcttagtaaaactgcaagattccaagggtaatgataccgatacatatgcgcttaaagatacaaatggcaatctttacgctgcggatgtgaatgaaactactggtgctgtttctgttaaaactattacctatactgactcttccggtgccgccagttctccaaccgcggtcaaactgggcggagatgatggcaaaacagaagtggtcgatattgatggtaaaacatacgattctgccgatttaaatggcggtaatctgcaaacaggtttgactgctggtggtgaggctctgactgctgttgcaaatggtaaaaccacggacccgctgaaagcgctggacgatgctatcgcatctgtagacaaattccgttcttccctcggtgcggtgcaaaaccgtctggattccgcggttaccaacctgaacaacaccactaccaacctgtctgaagcgcagtcccgtattcaggacgccgactatgcgaccgaagtgtccaatggttcgaaagcgcagatcatccagcaggccggtaactccgtgttggcaaaagctaaccaggtaccgcagcaggttctgtctctgctgcaaggttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z