##gff-version 3
##sequence-region BBa_K1617006 1 1357
BBa_K1617006	.	promoter	1	35	.	+	0	ID=BBa_J23106;Name=BBa_J23106
BBa_K1617006	.	CDS	62	1357	.	+	0	ID=BBa_K1617000;Name=BBa_K1617000
BBa_K1617006	.	point_mutation	612	612	.	+	0	ID=annotation2457251;Name=Delete BamHI site;Parent=BBa_K1617000
BBa_K1617006	.	point_mutation	723	723	.	+	0	ID=annotation2457280;Name=s_mutation;Parent=BBa_K1617000
BBa_K1617006	.	point_mutation	774	774	.	+	0	ID=annotation2457282;Name=Delete Pst1 site;Parent=BBa_K1617000
BBa_K1617006	.	engineered_region	638	720	.	+	0	ID=annotation2457075;Name=BBa_K1617010;Parent=BBa_K1617000
BBa_K1617006	.	sequence_feature	491	493	.	+	0	ID=annotation2457073;Name=M144A;Parent=BBa_K1617000
BBa_K1617006	.	point_mutation	1077	1077	.	+	0	ID=annotation2457283;Name=Delete Spe1 site;Parent=BBa_K1617000
BBa_K1617006	.	point_mutation	366	366	.	+	0	ID=annotation2457241;Name=Delete Pst1 site;Parent=BBa_K1617000
BBa_K1617006	.	sequence_feature	1264	1266	.	+	0	ID=annotation2457232;Name=M401G;Parent=BBa_K1617000
BBa_K1617006	.	sequence_feature	638	720	.	+	0	ID=annotation2457215;Name=MCS;Parent=BBa_K1617000
BBa_K1617006	.	point_mutation	1351	1351	.	+	0	ID=annotation2457286;Name=Delete Pst1 site;Parent=BBa_K1617000
BBa_K1617006	.	start_codon	62	64	.	+	0	ID=annotation2457065;Name=Start ATG;Parent=BBa_K1617000
BBa_K1617006	.	point_mutation	288	288	.	+	0	ID=annotation2457231;Name=Delete Xba1 site;Parent=BBa_K1617000
BBa_K1617006	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1617006	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K1617006
tttacggctagctcagtcctaggtatagtgctagctactagagaaagaggagaaatactagatggcacaagtcattaat
accaacagcctctcgctgatcactcaaaataatatcaacaagaaccagtctgcgctgtcgagttctatcgagcgtctgt
cttctggcttgcgtattaacagcgcgaaggatgacgcagcgggtcaggcgattgctaaccgtttcacctctaacattaa
aggcctgactcaggcggcccgtaacgccaacgacggtatctccgttgcccagaccaccgaaggcgcgctgtccgaaatc
aacaacaacttacagcgtgtgcgtgaactgacggtacaggccactaccggcactaactctgagtctgatctgtcttcta
tccaggacgaaattaaatcccgtctggatgaaattgaccgcgtatctggtcagacccagttcaacggcgtgaacgtgct
ggcaaaaaatggctccgcaaaaatccaggttggcgcaaatgataaccagactatcactatcgatctgaagcagattgat
gctaaaactcttggccttgatggttttagcgttaaaaataacgatacagttaccaccagtgctccagtaactgcttttg
gtgctggatccgccggcgtcgacatgggtggcagcatgggtggcagcatgaagcttagatctaccggttcaggcggtac
acctgttcagattgataataccgcaggttccgcaactgccaaccttggtgctgttagcttagtaaaactgcaagattcc
aagggtaatgataccgatacatatgcgcttaaagatacaaatggcaatctttacgctgcggatgtgaatgaaactactg
gtgctgtttctgttaaaactattacctatactgactcttccggtgccgccagttctccaaccgcggtcaaactgggcgg
agatgatggcaaaacagaagtggtcgatattgatggtaaaacatacgattctgccgatttaaatggcggtaatctgcaa
acaggtttgactgctggtggtgaggctctgactgctgttgcaaatggtaaaaccacggacccgctgaaagcgctggacg
atgctatcgcatctgtagacaaattccgttcttccctcggtgcggtgcaaaaccgtctggattccgcggttaccaacct
gaacaacaccactaccaacctgtctgaagcgcagtcccgtattcaggacgccgactatgcgaccgaagtgtccaatggt
tcgaaagcgcagatcatccagcaggccggtaactccgtgttggcaaaagctaaccaggtaccgcagcaggttctgtctc
tgctgcaaggttaa