LOCUS       BBa_K1618020            2197 bp    DNA     linear       12-May-2024
DEFINITION  Plac-Buk-PTB
ACCESSION   BBa_K1618020
VERSION     BBa_K1618020.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..1297
                     /label=BBa_K1618038
     CDS             1304..2197
                     /label=BBa_K1618039
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt atagattact
      241 aataatcaat cctggctcga cctcaactaa aattggtatt tatgacgatg aaaaagagat
      301 atttgagaaa actttaagac attcagctga agagatagaa aaatataaca ctatatttga
      361 tcaatttcaa ttcagaaaga atgtaatttt agatgcgtta aaagaagcaa acatagaagt
      421 aagttcttta aatgctgtag ttggaagagg cggactctta aagccaatag taagtggaac
      481 ttatgcagta aatcaaaaaa tgcttgaaga tcttaaagta ggagttcaag gtcagcatgc
      541 gtcaaatctt ggtggaatta ttgcaaatga aatagcaaaa gaaataaatg ttccagcata
      601 catagttgat ccagttgttg tggatgagct tgatgaagtt tcaagaatat caggaatggc
      661 tgacattcca agaaaaagta tattccatgc attaaatcaa aaagcagttg ctagaagata
      721 tgcaaaagaa gttggaaaaa aatacgaaga tcttaattta atcgtagtcc acatgggtgg
      781 aggtacttca gtaggtactc ataaagatgg tagagtaata gaagttaata atacacttga
      841 tggagaaggt ccattctcac cagaaagaag tggtggagtt ccaataggag atcttgtaag
      901 attgtgcttc agcaacaaat atacttatga agaagtaatg aaaaagataa acggcaaagg
      961 cggagttgtt agttacttaa atactatcga ttttaaggct gtagttgata aagctcttga
     1021 aggagataag aaatgtgcac ttatatatga agctttcaca ttccaggtag caaaagagat
     1081 aggaaaatgt tcaaccgttt taaaaggaaa tgtagatgca ataatcttaa caggcggaat
     1141 tgcgtacaac gagcatgtat gtaatgccat agaggataga gtaaaattca tagcacctgt
     1201 agttagatat ggtggagaag atgaacttct tgcacttcga gaaggtggac ttagagtttt
     1261 aagaggagaa gaaaaagcta aggaatacaa ataataatac tagatgatta agagttttaa
     1321 tgaaattatc atgaaggtaa agagcaaaga aatgaaaaaa gttgctgttg ctgtagcaca
     1381 agacgagcca gtacttgaag cagtaagaga tgctaagaaa aatggtattg cagatgctat
     1441 tcttgttgga gaccatgacg aaatcgtgtc aatcgcgctt aaaataggaa tggatgtaaa
     1501 tgattttgaa atagtaaacg agcctaacgt taagaaagct gctttaaagg cagtagagct
     1561 tgtatcaact ggaaaagctg atatattaat gaatggactt gtaaatacag caactttctt
     1621 aaagatctgc atattaaaca aagaagttgg acttagaaca ggaaaaacta tgtctcacgt
     1681 tgcagtattt gaaactgaga catctgatag actatcattt ttaacagatg ttgctttcaa
     1741 tacttatcct gaattaaagg aaaaaattga tatagtaaac aattcagtta aggttgcaca
     1801 tgcaatagga attgtaaatc caaaggttgc tccaatttgt gcagttgagg ttataaaccc
     1861 taaaatgcca tcaacacttg atgcagcaat gctttcaaaa atgagtgaca gaggacaaat
     1921 taaaggttgt gtagttgacg gacctttagc acttgatata gctttatcag aagaagcagc
     1981 acatcataag ggagtaacag gagaagttgc tggaaaagct gatatcttct taatgccaaa
     2041 catagaaaca ggaaatgtaa tgtataagac tttaacatat acaactgatt caaaaaatgg
     2101 aggaatctta gttggaactt ctgcaccagt tgttttaact tcaagagctg acagccatga
     2161 aacaaaaatg aactctatag cacttgcagc ttaataa
//