LOCUS BBa_K1618021 6101 bp DNA linear 22-Nov-2024 DEFINITION Plac-THL-CRO-BHBD-BCD-EFTb-EFTa ACCESSION BBa_K1618021 VERSION BBa_K1618021.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 misc_feature 1..220 /label=BBa_J04500 CDS 229..1430 /label=BBa_K1618040 CDS 1437..2306 /label=BBa_K1618041 CDS 2313..3101 /label=BBa_K1618042 CDS 3108..4271 /label=BBa_K1618043 CDS 4278..5060 /label=BBa_K1618044 CDS 5067..6101 /label=BBa_K1618045 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagga attcgcggcc 241 gcttctagat ggcgaaaaaa atcgtactgg caggtgcgtg ccgtacggca atcggcacca 301 tgggcggcag cctgtcaacc acgccagcgc cggtattagg ttccatcgta attaaggagg 361 cgctgaaccg tgccggagtg ccagctgatc aggtcgatca cgtgtatatg ggctgcgtga 421 tccaagctgg attagggcag aatgttgccc gccaggcttc gatcaaagcc ggtttgccca 481 tcgagacgac ggcggtaacg gtgaacgttg tctgcggttc tggcctgaat tgcgtaaaca 541 tggcggccca gatgattgaa gcgggcgatg cggacattgt tgttgcaggt ggaatggaaa 601 atatgtccat ggcgccgtac gccgtcatgc agggccgttt cggatatcgc atgaatgatg 661 gcaaactggt ggacaccatg gtgcatgatg ccttgtggga cgcatttaat gattatcaca 721 tgatcaaaac cgcagataac atttgtgaac agtgggggtt aacccgcgaa gaactggatg 781 agttcgccgc taaatcacag cagaaagcag tcgcggcaca ggaaagcggg gccttcgacg 841 ctgaaatcgt cccggttatg gttaaaaaaa aaaaagaaat ggtagaattt aaaaaagatg 901 aaggcccgcg tcccggtaca gtggataagc aggtcaaccc aaatggcggg gcgattgccc 961 tgggtcatcc cgttggcgca tccggctgtc gcatcttagt gacgctgctg cacgaaatgc 1021 aggccaaagg tgccaaaaca ggactggcaa ccctgtgtat cggcggcggt atgggctgta 1081 gcacggtcgt taagattgag gataccgtag aaacgttatc ccgtctgcgc actattaacc 1141 cgaatggctt tgtgaccgcg ggcaatgcta gcggaattaa tgatggtgca gccgcgatcg 1201 tcgttatgag cgaagagaaa gctaaagaac tcggcgtgaa accaatggcg acgtgggtgg 1261 caggtgcact gggcggtgtt gacccctcga ttatgggtat tggcccggtg gcatcgacta 1321 agaaagttat ggccaaaacc ggtctgaaaa tcgaggactt cgacattatt gaagcgaacg 1381 aagcatttgc ggcgcagtct gttgcggtgg ccaaagatct gggctttgat tactagatga 1441 aagtgggtgt tatcggtgct ggcaccatgg gccagggcat tgcaaaagct ttcgcccaag 1501 ttgacggtta tgaagtagcg ctgtgcgata ttaaacaaga atgggcagaa ggtggaaaag 1561 ataaaatcgc gaaaggatat gcacggttgg tcgagaaagg caaaatgacg caagagaaag 1621 tggatggcat tttggcctcg attacaccgg gcatcaaaga ggatctctgt gccgattgcg 1681 atctgattgt cgaagccgcg tttgaaaata tggaagtcaa aaagaccaca ttttcggaac 1741 tggacaagat ttgtaaaccg gaatgcgtgt tcgcgtccaa taccagttct ttgtcaatca 1801 ccgaaattgg caatggctta actcgcccaa tgatcggtat gcacttcttc aatccggccg 1861 atcgtatgaa attgatcgaa gttatcgcgg gtgttaacac cccagcagaa accgtcgata 1921 aaattgtgaa aatttctgaa gaaattggta aaactcctgt gcaagtaaat gaagccgcag 1981 gattcgtggt gaaccgcatt ctgattccga tgatcaatga ggcggcgttt atcaaaatgg 2041 aaggcgtcag tgacattgcg ggtatcgatg cagctatgaa actgggtgcc aaccatccga 2101 tgggaccgct ggaactggga gattttgtgg ggctggatat ctgtctggca attatggatg 2161 tgctttataa cgaaacgggt gattcgaaat accgcgcatg cccgcttctc cgcaaaatgg 2221 tacgtggtgg taatctgggc gttaaatctg gtaaaggctt ttacgtctac aacgccgacc 2281 gtactaaaac tccagtggac gcgcagtact agatggaatt tgttacctat gagcaagatg 2341 gctttgttgg agttattacg atcaatcgcc caaaagcgct gaacgcgctg aatagcaccg 2401 tgctcgaaga actcgatgcg accttcaaag ctgtggacct gaatacaact cgctgtctcg 2461 tcttgactgg tgcaggcgaa aaaagcttcg tcgccggtgc cgacatcggg gaaatgagta 2521 cgctgacgaa agcagaaggg gaagcatttg gtaaaaaagg taatgatgtg ttccgcatga 2581 tcgagacttt tccgattcca gtcatcgccg cggttaacgg cttcgctctg ggcggcggtt 2641 gcgagatttc aatgagttgt gatatccgta tttgttccga aaacgccgtg tttgggcagc 2701 cagaagtggg tctgggcatc acacctggtt ttggcgggac gcagcgcctg gcgcgcttag 2761 tcggggcggg catggcaaaa caacttattt ataccgcccg caatatcaag gccgatgagg 2821 cataccgtat tgggcttgtc aacgctgtct atccgctgga agagttgctt cctgccgcga 2881 aaaaaatggc cagtattatt gctgccaatg caccgattgc tgtgcgcaat tgtaaaaaag 2941 cgattaacga tggacttcaa gtggatatgg atcaagcgat cgtggtagag gaaaagttat 3001 ttggcgattg ttttgaaacc gaggaccaaa aagcgggtat gggtaacttt ctggagaaag 3061 acaaggaaaa aaaactgaaa gttgtcccct ttcagaacaa atactagatg gacttcgcct 3121 tagataaaaa gtacgaaatg gctcagaatt tattccgcga attcgcacag aatgaagtga 3181 aaccgctggc tcaggaaatc gatgaaaacc atcgctttcc gcgtgaaacc gttgagaaaa 3241 tggcaaaata cggctttctt gggattcccg tttcaaaaga ggacggcggc caaggctgcg 3301 acccccttac ctatgtcatg tgtgttgagg aacttagcaa ggtttgcggt acgaccggtg 3361 taatcgtgtc agcacatact agcctttgtg tagatccgat tcaaacctac ggcacgccgg 3421 accagaaagc taagtacctg ccggatctgg cttcaggccg taaattgggt gcctttggtc 3481 tgactgaacc aggagccggg acggacgccc agggccagca aaccaaagcc gttctggacg 3541 gggacgagtg ggtcctgaat ggttctaaat gttttatcac gaacggcaaa gaagccgacg 3601 tctatattat tattgcggtt acggggacta tcgaaaaacg cggccgtaaa caaaaagaaa 3661 tctccgcttt catcgttgaa aaggggactc ccggttttac ctttggtact aaagaaaata 3721 agatgggcat ttgcggcagc agcacatatg agctgatctt caccgactgc cgtattccga 3781 aggacgcgct gctgggtcaa aaaggcaaag gctttggtat tgccatgcat acactggacg 3841 gcggccgcat cggcattgcc gcgcaggcac tcggtattgc tgaaggtgca ctggacatca 3901 ccatcgatta cgttaaagaa cgtaagcaat tcggacgctc aatcgctcag ttccaaaaca 3961 cgcagtttgt gctggcagac ttggcaacaa aagtagaagc agccaaactg ctggtgtata 4021 aggccgcaaa aaagaaagaa caatatcaag cggatcataa aacaagctat agcgttgaag 4081 ccgctatggc caaactgtat gcagcggaag tggcaatgga agttacaacg aaatgcgtgc 4141 aactgcacgg cggatacggt tatatcaagg agtatgatgt agaacgcatg atgcgcgacg 4201 ctaaaattac tgaaatttac gagggcacta gcgaagttca acgtatggtt atttccgcta 4261 atctgctgaa atactagatg aaagtgattg tttgtattaa acaagtgcct gacaccaagg 4321 gcggcgttca gtttaatcct gatggtacgt tgaatcgggg ggcgatgctg acaatcatga 4381 acccggacga caaagcaggc ctggaagcgg ccctgcggct gaaggatcaa tatggggcag 4441 aagtcacggt gttaacgatg ggcctgccga aagcggcaga cgtacttcag gaagcgattg 4501 caatgggtgc agataaaggc gtgttggtta ccgaccgggt tctcggcgga gctgatacct 4561 gggccacatc gacaaccatc gccggtgctt tacgcaaact tgactacgat ctcattatca 4621 cgggtcgcca ggctattgat ggagatactg cacaagtggg tccgcagatc gcggagcatc 4681 tcggcattcc ggtgatttcg tacgcacagg aaattaaagt ggatggtgac tcggtgattg 4741 ttaaacgcca gtatgatgac ggctatcata tgctgaaagc taaaatgccc tgcctggtga 4801 cggcgctgtc agagctgaat gaaccgcgtt acatgacccc aggtgggatc tttgatgctg 4861 tgaatgccga aattactacc ctgggccgcg ccgatcttgt cgatgtggat gatagtaatt 4921 tgggtttgaa aggttccccg accaaaatcg cgaaagcttc agataaagtg cggaagggcg 4981 ccggtgaaaa agtcgttcca gattctccag acgaagccgt tagttacatc gtagggaaac 5041 tcaaagacaa acatgttatt tactagatga aaaactataa aggtgtattt gtgttcgcac 5101 aacaggtgga taacaagctt agcggtatct cgttcgaact gattggcgaa ggtaaacgtt 5161 tagctgataa attaaacgaa aaggttaccg cggtactgat cggcagcgat gttaaaggcc 5221 ttgtcgatga actggccgaa tatggggcag accgtgtgat cgtggttgat gatccggaat 5281 taaaggaata tcgtaccgaa ccatatgctc atgccctggc aagcgttatt aatgagtata 5341 agcccgaaat cgttctcgtc ggcgctacag cgattggccg tgacctgggg ccgacggtga 5401 gtgcgcgcgt tgccacggga ctgaccgcgg actgcaccct tctggaaatt ggtgacttcc 5461 cattagtcgc cttgcccaat caagaacaga aacacaacca attacttatg actcggcctg 5521 cctttggtgg aaacacgatt gcgacaattg cttgtcctga caatcgcccg caaatggcca 5581 ccgttcgtcc gggcgttatg caaaaacttg atcgtgtggc aggcaaaaaa gcagaggtca 5641 ttgagtataa tccgggtttt actcctaaca acaaatacgt cgaaatctta gaaatttcga 5701 aagcagtttc tgacattaaa gacatcatgg acgcgaaaat tctggtgtcg gggggacggg 5761 gcgtaggtag cgcagaaaat tttaagctgc tggatgatct ggccgaagtt ctgggtggcc 5821 aggtgtcctg ctcgcgtgcc gtagttgatt cgggttggaa gccgaaagaa ttgcaggttg 5881 ggcagaccgg gaaaaccgtg cgccctcagg tctatttcgc catcggcatc tcaggtgcta 5941 tccagcacgt ggccggtatg gaagaaagtg atattatcat cgccattaat aaagatgaag 6001 atgcaccgat tttcgacgtt gctgattacg gtgtggtagg cgatttaaat aaaatcgtac 6061 ccgcgttaac agaagccatt aaggctgagc tggcgaacaa a //