LOCUS       BBa_K1618022            2416 bp    DNA     linear       17-May-2024
DEFINITION  GlgB with IPTG-inducible promoter
ACCESSION   BBa_K1618022
VERSION     BBa_K1618022.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..2416
                     /label=BBa_K1618000
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt ccgatcgtat
      241 cgatagagat gtgattaacg cgctaattgc aggccatttt gcggatcctt tttccgtact
      301 gggaatgcat aaaaccaccg cgggactgga agtccgtgcc cttttacccg acgctaccga
      361 tgtgtgggtg attgaaccga aaaccgggcg caaactcgca aaactggagt gtctcgactc
      421 acggggattc tttagcggcg tcattccgcg acgtaagaat tttttccgct atcagttggc
      481 tgttgtctgg catggtcagc aaaacctgat tgatgatcct taccgttttg gtccgctaat
      541 ccaggaaatg gatgcctggc tattatctga aggtactcac ctgcgcccgt atgaaacctt
      601 aggcgcgcat gcagatacta tggatggcgt cacaggtacg cgtttctctg tctgggctcc
      661 aaacgcccgt cgggtgtcgg tggttgggca attcaactac tgggacggtc gccgtcaccc
      721 gatgcgcctg cgtaaagaga gcggcatctg ggaactgttt atccctgggg cgcataacgg
      781 tcagctctat aaatacgaga tgattgatgc caatggcaac ttgcgtctga agtccgaccc
      841 ttatgccttt gaagcgcaaa tgcgcccgga aaccgcgtca cttatttgcg ggctgccgga
      901 aaaggttgta cagactgaag agcgcaaaaa agcgaatcag tttgatgcgc caatctctat
      961 ttatgaagtt cacctgggtt cctggcgtcg ccacaccgac aacaatttct ggttgagcta
     1021 ccgcgagctg gccgatcaac tggtgcctta tgctaaatgg atgggcttta cccacctcga
     1081 actactgccc attaacgagc atcccttcga tggcagttgg ggttatcagc caaccggcct
     1141 gtatgcgcca acccgccgtt ttggtactcg cgacgacttc cgttatttca ttgatgccgc
     1201 acacgcagct ggtctgaacg tgattctcga ctgggtgcca ggccacttcc cgactgatga
     1261 ctttgcgctt gccgaatttg atggcacgaa cttgtatgaa cacagcgatc cgcgtgaagg
     1321 ctatcatcag gactggaaca cgctgatcta caactatggt cgccgtgaag tcagtaactt
     1381 cctcgtcggt aacgcgcttt actggattga acgttttggt attgatgcgc tgcgcgtcga
     1441 tgcggtggcg tcaatgattt atcgcgacta cagccgtaaa gagggggagt ggatcccgaa
     1501 cgaatttggc gggcgcgaga atcttgaagc gattgagttc ttgcgtaata ccaaccgtat
     1561 tcttggtgag caggtttccg gtgcggtgac aatggctgag gagtctaccg atttccctgg
     1621 cgtttctcgt ccgcaggata tgggcggtct gggcttctgg tacaagtgga acctcggctg
     1681 gatgcatgac accctggact acatgaagct cgacccggtt tatcgtcagt atcatcacga
     1741 taaactgacc ttcgggattc tctacaacta cactgaaaac ttcgtcctgc cgttgtcgca
     1801 tgatgaagtg gtccacggta aaaaatcgat tctcgaccgc atgccgggcg acgcatggca
     1861 gaaattcgcg aacctgcgcg cctactatgg ctggatgtgg gcattcccgg gcaagaaact
     1921 actgttcatg ggtaacgaat ttgcccaggg ccgcgagtgg aaccatgacg ccagcctcga
     1981 ctggcatctg ttggaaggcg gcgataactg gcaccacggt gtccagcgtc tggtgcgcga
     2041 tctgaacctc acctaccgcc accataaagc aatgcatgaa ctggattttg acccgtacgg
     2101 ctttgaatgg ctggtggtgg atgacaaaga acgctcggtg ctgatctttg tgcgtcgcga
     2161 taaagagggt aacgaaatca tcgttgccag taactttacg ccggtaccgc gtcatgatta
     2221 tcgcttcggc ataaaccagc cgggcaaatg gcgtgaaatc ctcaataccg attccatgca
     2281 ctatcacggc agtaatgcag gcaatggcgg cacggtacac agcgatgaga ttgccagcca
     2341 cggtcgtcag cattcactaa gcctgacgct accaccgctg gccactatct ggctggttcg
     2401 ggaggcagaa tgataa
//