BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961225 1 -10 range1961225 1 161 166 annotation1961227 1 start range1961227 1 173 173 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961224 1 -35 range1961224 1 137 142 BBa_J04500 1 BBa_J04500 IPTG inducible promoter with RBS 2005-06-08T11:00:00Z 2015-08-31T04:08:14Z Davidson Synth-Aces Released HQ 2013 R0010.B0034 false true _16_ 0 326 16 In stock false false Kristen DeCelle component1508149 1 BBa_R0010 component1508159 1 BBa_B0034 annotation1508159 1 BBa_B0034 range1508159 1 209 220 annotation1508149 1 BBa_R0010 range1508149 1 1 200 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1618023 1 ProGlgBRv3 GlgB-Rv3030 with IPTG-inducible promoter 2015-09-02T11:00:00Z 2015-09-16T02:57:39Z Bacterial sequence Rv3030 is a gene which codes for a putative acyltransferase which acylates glycogen/starch chains. This enzyme is encoded with GlgB which codes for a branching enzyme which will cut alpha-1,4 links and reattach the cut glycogen strand and re-anneal the strand to the main glycogen compound through an alpha-1,6 linkage, therefore creating a branch. These enzymes are promoted by an IPTG-inducible promoter and are encoded within the pSB1C3 standard vector. false false _2035_ 24779 24731 9 false None false Mark Elms Farhan Mitha component2461972 1 BBa_K1618002 component2461971 1 BBa_K1618022 annotation2461971 1 BBa_K1618022 range2461971 1 1 2416 annotation2461972 1 BBa_K1618002 range2461972 1 2423 3250 BBa_K1618000 1 GlgB GlgB without inducible promoter 2015-08-09T11:00:00Z 2015-09-16T01:24:53Z Bacterial genome GlgB is an enzyme that increases glycogen branching levels during glycogen synthesis in bacteria. false false _2035_ 24779 24623 9 false None false Mark Elms Farhan Mitha BBa_K1618002 1 Rv3030 Rv3030 without inducible promoter 2015-08-10T11:00:00Z 2015-09-16T11:10:37Z Bacterial sequence Rv3030 is a gene which encodes a possible acylation candidate which will acylate glycogen chains in bacteria. The gene is inserted within the pSB1C3 vector. false false _2035_ 24779 24623 9 false None false Farhan Mitha Mark Elms BBa_K1618022 1 ProGlgB GlgB with IPTG-inducible promoter 2015-08-29T11:00:00Z 2015-09-16T02:57:14Z Bacterial sequence. GlgB is a genetic sequence which codes for a glycogen branching enzyme which adds branches to glycogen strands to make the entire molecule more branched. The coding sequence is preceded by an IPTG-inducible promoter and both sequences are encoded within the pSB1C3 vector. false false _2035_ 24779 24731 9 false None false Mark Elms Farhan Mitha component2461959 1 BBa_J04500 component2461960 1 BBa_K1618000 annotation2461960 1 BBa_K1618000 range2461960 1 227 2416 annotation2461959 1 BBa_J04500 range2461959 1 1 220 BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_B0034_sequence 1 aaagaggagaaa BBa_K1618002_sequence 1 atgtgcgcctttgtgccgcacgtgcctcgccattcgcgcggagataatccacctagtgcgagtacggcttcgcctgcggttctgactttgactggagaacgtactattcccgatctggacattgaaaactactggtttcggcgccaccaagtagtgtaccagcgtctggctccgcgttgcaccgcacgtgatgttctggaagccggttgcggtgaaggttacggagcggatctcatcgcttgcgttgcccggcaagtgattgcggtcgactatgatgaaactgccgtggcacatgttcgtagccgctatccacgcgtcgaggtgatgcaggcgaacttagcagaactgccgctgcctgatgcgtctgtagacgtcgttgtgaactttcaagtcattgaacacctgtgggaccaggcgcgtttcgtccgcgagtgcgcacgggtactgcgtggctcgggcctgctcatggtgagtaccccgaatcgcattacgttttctccggggcgcgataccccgattaatccatttcatacccgcgaactgaatgcagatgaattaaccagcctgctgattgatgccggttttgtggatgtggccatgtgtggtctgtttcatggcccgcgccttcgggatatggatgcccggcatggaggcagcatcattgacgcgcagattatgcgtgctgtagcgggcgcgccttggccgccggagttggccgcggatgtagccgcagtcaccacggcggatttcgagatggtagcggcgggccatgaccgcgatattgatgacagtttagacttaattgccattgcggttcgtccataataa BBa_K1618023_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgtccgatcgtatcgatagagatgtgattaacgcgctaattgcaggccattttgcggatcctttttccgtactgggaatgcataaaaccaccgcgggactggaagtccgtgcccttttacccgacgctaccgatgtgtgggtgattgaaccgaaaaccgggcgcaaactcgcaaaactggagtgtctcgactcacggggattctttagcggcgtcattccgcgacgtaagaattttttccgctatcagttggctgttgtctggcatggtcagcaaaacctgattgatgatccttaccgttttggtccgctaatccaggaaatggatgcctggctattatctgaaggtactcacctgcgcccgtatgaaaccttaggcgcgcatgcagatactatggatggcgtcacaggtacgcgtttctctgtctgggctccaaacgcccgtcgggtgtcggtggttgggcaattcaactactgggacggtcgccgtcacccgatgcgcctgcgtaaagagagcggcatctgggaactgtttatccctggggcgcataacggtcagctctataaatacgagatgattgatgccaatggcaacttgcgtctgaagtccgacccttatgcctttgaagcgcaaatgcgcccggaaaccgcgtcacttatttgcgggctgccggaaaaggttgtacagactgaagagcgcaaaaaagcgaatcagtttgatgcgccaatctctatttatgaagttcacctgggttcctggcgtcgccacaccgacaacaatttctggttgagctaccgcgagctggccgatcaactggtgccttatgctaaatggatgggctttacccacctcgaactactgcccattaacgagcatcccttcgatggcagttggggttatcagccaaccggcctgtatgcgccaacccgccgttttggtactcgcgacgacttccgttatttcattgatgccgcacacgcagctggtctgaacgtgattctcgactgggtgccaggccacttcccgactgatgactttgcgcttgccgaatttgatggcacgaacttgtatgaacacagcgatccgcgtgaaggctatcatcaggactggaacacgctgatctacaactatggtcgccgtgaagtcagtaacttcctcgtcggtaacgcgctttactggattgaacgttttggtattgatgcgctgcgcgtcgatgcggtggcgtcaatgatttatcgcgactacagccgtaaagagggggagtggatcccgaacgaatttggcgggcgcgagaatcttgaagcgattgagttcttgcgtaataccaaccgtattcttggtgagcaggtttccggtgcggtgacaatggctgaggagtctaccgatttccctggcgtttctcgtccgcaggatatgggcggtctgggcttctggtacaagtggaacctcggctggatgcatgacaccctggactacatgaagctcgacccggtttatcgtcagtatcatcacgataaactgaccttcgggattctctacaactacactgaaaacttcgtcctgccgttgtcgcatgatgaagtggtccacggtaaaaaatcgattctcgaccgcatgccgggcgacgcatggcagaaattcgcgaacctgcgcgcctactatggctggatgtgggcattcccgggcaagaaactactgttcatgggtaacgaatttgcccagggccgcgagtggaaccatgacgccagcctcgactggcatctgttggaaggcggcgataactggcaccacggtgtccagcgtctggtgcgcgatctgaacctcacctaccgccaccataaagcaatgcatgaactggattttgacccgtacggctttgaatggctggtggtggatgacaaagaacgctcggtgctgatctttgtgcgtcgcgataaagagggtaacgaaatcatcgttgccagtaactttacgccggtaccgcgtcatgattatcgcttcggcataaaccagccgggcaaatggcgtgaaatcctcaataccgattccatgcactatcacggcagtaatgcaggcaatggcggcacggtacacagcgatgagattgccagccacggtcgtcagcattcactaagcctgacgctaccaccgctggccactatctggctggttcgggaggcagaatgataatactagatgtgcgcctttgtgccgcacgtgcctcgccattcgcgcggagataatccacctagtgcgagtacggcttcgcctgcggttctgactttgactggagaacgtactattcccgatctggacattgaaaactactggtttcggcgccaccaagtagtgtaccagcgtctggctccgcgttgcaccgcacgtgatgttctggaagccggttgcggtgaaggttacggagcggatctcatcgcttgcgttgcccggcaagtgattgcggtcgactatgatgaaactgccgtggcacatgttcgtagccgctatccacgcgtcgaggtgatgcaggcgaacttagcagaactgccgctgcctgatgcgtctgtagacgtcgttgtgaactttcaagtcattgaacacctgtgggaccaggcgcgtttcgtccgcgagtgcgcacgggtactgcgtggctcgggcctgctcatggtgagtaccccgaatcgcattacgttttctccggggcgcgataccccgattaatccatttcatacccgcgaactgaatgcagatgaattaaccagcctgctgattgatgccggttttgtggatgtggccatgtgtggtctgtttcatggcccgcgccttcgggatatggatgcccggcatggaggcagcatcattgacgcgcagattatgcgtgctgtagcgggcgcgccttggccgccggagttggccgcggatgtagccgcagtcaccacggcggatttcgagatggtagcggcgggccatgaccgcgatattgatgacagtttagacttaattgccattgcggttcgtccataataa BBa_K1618000_sequence 1 atgtccgatcgtatcgatagagatgtgattaacgcgctaattgcaggccattttgcggatcctttttccgtactgggaatgcataaaaccaccgcgggactggaagtccgtgcccttttacccgacgctaccgatgtgtgggtgattgaaccgaaaaccgggcgcaaactcgcaaaactggagtgtctcgactcacggggattctttagcggcgtcattccgcgacgtaagaattttttccgctatcagttggctgttgtctggcatggtcagcaaaacctgattgatgatccttaccgttttggtccgctaatccaggaaatggatgcctggctattatctgaaggtactcacctgcgcccgtatgaaaccttaggcgcgcatgcagatactatggatggcgtcacaggtacgcgtttctctgtctgggctccaaacgcccgtcgggtgtcggtggttgggcaattcaactactgggacggtcgccgtcacccgatgcgcctgcgtaaagagagcggcatctgggaactgtttatccctggggcgcataacggtcagctctataaatacgagatgattgatgccaatggcaacttgcgtctgaagtccgacccttatgcctttgaagcgcaaatgcgcccggaaaccgcgtcacttatttgcgggctgccggaaaaggttgtacagactgaagagcgcaaaaaagcgaatcagtttgatgcgccaatctctatttatgaagttcacctgggttcctggcgtcgccacaccgacaacaatttctggttgagctaccgcgagctggccgatcaactggtgccttatgctaaatggatgggctttacccacctcgaactactgcccattaacgagcatcccttcgatggcagttggggttatcagccaaccggcctgtatgcgccaacccgccgttttggtactcgcgacgacttccgttatttcattgatgccgcacacgcagctggtctgaacgtgattctcgactgggtgccaggccacttcccgactgatgactttgcgcttgccgaatttgatggcacgaacttgtatgaacacagcgatccgcgtgaaggctatcatcaggactggaacacgctgatctacaactatggtcgccgtgaagtcagtaacttcctcgtcggtaacgcgctttactggattgaacgttttggtattgatgcgctgcgcgtcgatgcggtggcgtcaatgatttatcgcgactacagccgtaaagagggggagtggatcccgaacgaatttggcgggcgcgagaatcttgaagcgattgagttcttgcgtaataccaaccgtattcttggtgagcaggtttccggtgcggtgacaatggctgaggagtctaccgatttccctggcgtttctcgtccgcaggatatgggcggtctgggcttctggtacaagtggaacctcggctggatgcatgacaccctggactacatgaagctcgacccggtttatcgtcagtatcatcacgataaactgaccttcgggattctctacaactacactgaaaacttcgtcctgccgttgtcgcatgatgaagtggtccacggtaaaaaatcgattctcgaccgcatgccgggcgacgcatggcagaaattcgcgaacctgcgcgcctactatggctggatgtgggcattcccgggcaagaaactactgttcatgggtaacgaatttgcccagggccgcgagtggaaccatgacgccagcctcgactggcatctgttggaaggcggcgataactggcaccacggtgtccagcgtctggtgcgcgatctgaacctcacctaccgccaccataaagcaatgcatgaactggattttgacccgtacggctttgaatggctggtggtggatgacaaagaacgctcggtgctgatctttgtgcgtcgcgataaagagggtaacgaaatcatcgttgccagtaactttacgccggtaccgcgtcatgattatcgcttcggcataaaccagccgggcaaatggcgtgaaatcctcaataccgattccatgcactatcacggcagtaatgcaggcaatggcggcacggtacacagcgatgagattgccagccacggtcgtcagcattcactaagcctgacgctaccaccgctggccactatctggctggttcgggaggcagaatgataa BBa_J04500_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa BBa_K1618022_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgtccgatcgtatcgatagagatgtgattaacgcgctaattgcaggccattttgcggatcctttttccgtactgggaatgcataaaaccaccgcgggactggaagtccgtgcccttttacccgacgctaccgatgtgtgggtgattgaaccgaaaaccgggcgcaaactcgcaaaactggagtgtctcgactcacggggattctttagcggcgtcattccgcgacgtaagaattttttccgctatcagttggctgttgtctggcatggtcagcaaaacctgattgatgatccttaccgttttggtccgctaatccaggaaatggatgcctggctattatctgaaggtactcacctgcgcccgtatgaaaccttaggcgcgcatgcagatactatggatggcgtcacaggtacgcgtttctctgtctgggctccaaacgcccgtcgggtgtcggtggttgggcaattcaactactgggacggtcgccgtcacccgatgcgcctgcgtaaagagagcggcatctgggaactgtttatccctggggcgcataacggtcagctctataaatacgagatgattgatgccaatggcaacttgcgtctgaagtccgacccttatgcctttgaagcgcaaatgcgcccggaaaccgcgtcacttatttgcgggctgccggaaaaggttgtacagactgaagagcgcaaaaaagcgaatcagtttgatgcgccaatctctatttatgaagttcacctgggttcctggcgtcgccacaccgacaacaatttctggttgagctaccgcgagctggccgatcaactggtgccttatgctaaatggatgggctttacccacctcgaactactgcccattaacgagcatcccttcgatggcagttggggttatcagccaaccggcctgtatgcgccaacccgccgttttggtactcgcgacgacttccgttatttcattgatgccgcacacgcagctggtctgaacgtgattctcgactgggtgccaggccacttcccgactgatgactttgcgcttgccgaatttgatggcacgaacttgtatgaacacagcgatccgcgtgaaggctatcatcaggactggaacacgctgatctacaactatggtcgccgtgaagtcagtaacttcctcgtcggtaacgcgctttactggattgaacgttttggtattgatgcgctgcgcgtcgatgcggtggcgtcaatgatttatcgcgactacagccgtaaagagggggagtggatcccgaacgaatttggcgggcgcgagaatcttgaagcgattgagttcttgcgtaataccaaccgtattcttggtgagcaggtttccggtgcggtgacaatggctgaggagtctaccgatttccctggcgtttctcgtccgcaggatatgggcggtctgggcttctggtacaagtggaacctcggctggatgcatgacaccctggactacatgaagctcgacccggtttatcgtcagtatcatcacgataaactgaccttcgggattctctacaactacactgaaaacttcgtcctgccgttgtcgcatgatgaagtggtccacggtaaaaaatcgattctcgaccgcatgccgggcgacgcatggcagaaattcgcgaacctgcgcgcctactatggctggatgtgggcattcccgggcaagaaactactgttcatgggtaacgaatttgcccagggccgcgagtggaaccatgacgccagcctcgactggcatctgttggaaggcggcgataactggcaccacggtgtccagcgtctggtgcgcgatctgaacctcacctaccgccaccataaagcaatgcatgaactggattttgacccgtacggctttgaatggctggtggtggatgacaaagaacgctcggtgctgatctttgtgcgtcgcgataaagagggtaacgaaatcatcgttgccagtaactttacgccggtaccgcgtcatgattatcgcttcggcataaaccagccgggcaaatggcgtgaaatcctcaataccgattccatgcactatcacggcagtaatgcaggcaatggcggcacggtacacagcgatgagattgccagccacggtcgtcagcattcactaagcctgacgctaccaccgctggccactatctggctggttcgggaggcagaatgataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z