BBa_K1618022
1
ProGlgB
GlgB with IPTG-inducible promoter
2015-08-29T11:00:00Z
2015-09-16T02:57:14Z
Bacterial sequence.
GlgB is a genetic sequence which codes for a glycogen branching enzyme which adds branches to glycogen strands to make the entire molecule more branched. The coding sequence is preceded by an IPTG-inducible promoter and both sequences are encoded within the pSB1C3 vector.
false
false
_2035_
24779
24731
9
false
None
false
Mark Elms Farhan Mitha
component2461960
1
BBa_K1618000
component2461959
1
BBa_J04500
annotation2461959
1
BBa_J04500
range2461959
1
1
220
annotation2461960
1
BBa_K1618000
range2461960
1
227
2416
BBa_K1618024
1
BBa_K1618024
Pro-GlgB-Rv3034c
2015-09-02T11:00:00Z
2015-09-16T02:58:02Z
Bacterial sequence
Rv3034c is a gene which codes for a putative acyltransferase which acylates glycogen/starch chains. This enzyme is encoded with GlgB which codes for a branching enzyme which will cut alpha-1,4 links and reattach the cut glycogen strand and re-anneal the strand to the main glycogen compound through an alpha-1,6 linkage, therefore creating a branch. These enzymes are promoted by an IPTG-inducible promoter and are encoded within the pSB1C3 standard vector.
false
false
_2035_
24779
24731
9
false
None
false
Mark Elms
component2461984
1
BBa_K1618003
component2461983
1
BBa_K1618022
annotation2461984
1
BBa_K1618003
range2461984
1
2423
3328
annotation2461983
1
BBa_K1618022
range2461983
1
1
2416
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_J04500
1
BBa_J04500
IPTG inducible promoter with RBS
2005-06-08T11:00:00Z
2015-08-31T04:08:14Z
Davidson Synth-Aces
Released HQ 2013
R0010.B0034
false
true
_16_
0
326
16
In stock
false
false
Kristen DeCelle
component1508149
1
BBa_R0010
component1508159
1
BBa_B0034
annotation1508149
1
BBa_R0010
range1508149
1
1
200
annotation1508159
1
BBa_B0034
range1508159
1
209
220
BBa_K1618000
1
GlgB
GlgB without inducible promoter
2015-08-09T11:00:00Z
2015-09-16T01:24:53Z
Bacterial genome
GlgB is an enzyme that increases glycogen branching levels during glycogen synthesis in bacteria.
false
false
_2035_
24779
24623
9
false
None
false
Mark Elms Farhan Mitha
BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961227
1
start
range1961227
1
173
173
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961225
1
-10
range1961225
1
161
166
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961224
1
-35
range1961224
1
137
142
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
BBa_K1618003
1
Rv3034c
Rv3034c without inducible promoter
2015-08-10T11:00:00Z
2015-09-16T11:12:25Z
Bacterial sequence
Rv3034c is a genetic sequence which codes for a possible acylation candidate protein which will acylate glycogen chains in bacteria. The gene is encoded in the pSB1C3 vector.
false
false
_2035_
24779
24623
9
false
None
false
Farhan Mitha Mark Elms
BBa_K1618024_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgtccgatcgtatcgatagagatgtgattaacgcgctaattgcaggccattttgcggatcctttttccgtactgggaatgcataaaaccaccgcgggactggaagtccgtgcccttttacccgacgctaccgatgtgtgggtgattgaaccgaaaaccgggcgcaaactcgcaaaactggagtgtctcgactcacggggattctttagcggcgtcattccgcgacgtaagaattttttccgctatcagttggctgttgtctggcatggtcagcaaaacctgattgatgatccttaccgttttggtccgctaatccaggaaatggatgcctggctattatctgaaggtactcacctgcgcccgtatgaaaccttaggcgcgcatgcagatactatggatggcgtcacaggtacgcgtttctctgtctgggctccaaacgcccgtcgggtgtcggtggttgggcaattcaactactgggacggtcgccgtcacccgatgcgcctgcgtaaagagagcggcatctgggaactgtttatccctggggcgcataacggtcagctctataaatacgagatgattgatgccaatggcaacttgcgtctgaagtccgacccttatgcctttgaagcgcaaatgcgcccggaaaccgcgtcacttatttgcgggctgccggaaaaggttgtacagactgaagagcgcaaaaaagcgaatcagtttgatgcgccaatctctatttatgaagttcacctgggttcctggcgtcgccacaccgacaacaatttctggttgagctaccgcgagctggccgatcaactggtgccttatgctaaatggatgggctttacccacctcgaactactgcccattaacgagcatcccttcgatggcagttggggttatcagccaaccggcctgtatgcgccaacccgccgttttggtactcgcgacgacttccgttatttcattgatgccgcacacgcagctggtctgaacgtgattctcgactgggtgccaggccacttcccgactgatgactttgcgcttgccgaatttgatggcacgaacttgtatgaacacagcgatccgcgtgaaggctatcatcaggactggaacacgctgatctacaactatggtcgccgtgaagtcagtaacttcctcgtcggtaacgcgctttactggattgaacgttttggtattgatgcgctgcgcgtcgatgcggtggcgtcaatgatttatcgcgactacagccgtaaagagggggagtggatcccgaacgaatttggcgggcgcgagaatcttgaagcgattgagttcttgcgtaataccaaccgtattcttggtgagcaggtttccggtgcggtgacaatggctgaggagtctaccgatttccctggcgtttctcgtccgcaggatatgggcggtctgggcttctggtacaagtggaacctcggctggatgcatgacaccctggactacatgaagctcgacccggtttatcgtcagtatcatcacgataaactgaccttcgggattctctacaactacactgaaaacttcgtcctgccgttgtcgcatgatgaagtggtccacggtaaaaaatcgattctcgaccgcatgccgggcgacgcatggcagaaattcgcgaacctgcgcgcctactatggctggatgtgggcattcccgggcaagaaactactgttcatgggtaacgaatttgcccagggccgcgagtggaaccatgacgccagcctcgactggcatctgttggaaggcggcgataactggcaccacggtgtccagcgtctggtgcgcgatctgaacctcacctaccgccaccataaagcaatgcatgaactggattttgacccgtacggctttgaatggctggtggtggatgacaaagaacgctcggtgctgatctttgtgcgtcgcgataaagagggtaacgaaatcatcgttgccagtaactttacgccggtaccgcgtcatgattatcgcttcggcataaaccagccgggcaaatggcgtgaaatcctcaataccgattccatgcactatcacggcagtaatgcaggcaatggcggcacggtacacagcgatgagattgccagccacggtcgtcagcattcactaagcctgacgctaccaccgctggccactatctggctggttcgggaggcagaatgataatactagatgaatgtgttgagtttaggcagctctagtggtgttgtctggggccgtgtaccgattaccgccccggctggagctgcgaccggcgttacctcacgcgcggacgctcactcccagatgcgccgctatgcacagaccgggcctactgcgaaactgagttcagctccgatgaccacaatgtggggcgcgccgctccaccggcgttggcgcggaagtcgcttgcgggatccgcgtcaggcgaaatttttaacgctggcatcccttaagtgggtacttgcgaaccgtgcgtacactccttggtacttggtccgttactggcgtctgctgcgtttcaaactggcaaatcctcatattattactcgcggcatggtctttctcggtaaaggtgttgagatccatgcaacaccggaattagcgcagttagaaattggccgttgggtgcatattggcgataagaataccattcgcgctcatgagggttcgctccgcttcggtgacaaggtggtgttgggtcgcgacaacgttatcaatacataccttgacattgagatcggcgatagtgtactgatggcggactggtgctatatttgcgatttcgaccatcgcatggatgacatcacactgccaattaaagatcaggggatcattaaaagtccagttcgcattgggccggatacctggatcggggtcaaggtatctgttctgcgtgggacaacgattggccgtggatgtgtcttgggatcgcacgccgtggtgcgtggtgctatcccggactactcgattgctgtgggcgcaccggcaaaagtggttaagaatcgtcagctgtcgtgggaagcgtctgccgcacagcgggccgaactggcggcggcattagctgatatcgaacgcaaaaaagccgcccggtaataa
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1618000_sequence
1
atgtccgatcgtatcgatagagatgtgattaacgcgctaattgcaggccattttgcggatcctttttccgtactgggaatgcataaaaccaccgcgggactggaagtccgtgcccttttacccgacgctaccgatgtgtgggtgattgaaccgaaaaccgggcgcaaactcgcaaaactggagtgtctcgactcacggggattctttagcggcgtcattccgcgacgtaagaattttttccgctatcagttggctgttgtctggcatggtcagcaaaacctgattgatgatccttaccgttttggtccgctaatccaggaaatggatgcctggctattatctgaaggtactcacctgcgcccgtatgaaaccttaggcgcgcatgcagatactatggatggcgtcacaggtacgcgtttctctgtctgggctccaaacgcccgtcgggtgtcggtggttgggcaattcaactactgggacggtcgccgtcacccgatgcgcctgcgtaaagagagcggcatctgggaactgtttatccctggggcgcataacggtcagctctataaatacgagatgattgatgccaatggcaacttgcgtctgaagtccgacccttatgcctttgaagcgcaaatgcgcccggaaaccgcgtcacttatttgcgggctgccggaaaaggttgtacagactgaagagcgcaaaaaagcgaatcagtttgatgcgccaatctctatttatgaagttcacctgggttcctggcgtcgccacaccgacaacaatttctggttgagctaccgcgagctggccgatcaactggtgccttatgctaaatggatgggctttacccacctcgaactactgcccattaacgagcatcccttcgatggcagttggggttatcagccaaccggcctgtatgcgccaacccgccgttttggtactcgcgacgacttccgttatttcattgatgccgcacacgcagctggtctgaacgtgattctcgactgggtgccaggccacttcccgactgatgactttgcgcttgccgaatttgatggcacgaacttgtatgaacacagcgatccgcgtgaaggctatcatcaggactggaacacgctgatctacaactatggtcgccgtgaagtcagtaacttcctcgtcggtaacgcgctttactggattgaacgttttggtattgatgcgctgcgcgtcgatgcggtggcgtcaatgatttatcgcgactacagccgtaaagagggggagtggatcccgaacgaatttggcgggcgcgagaatcttgaagcgattgagttcttgcgtaataccaaccgtattcttggtgagcaggtttccggtgcggtgacaatggctgaggagtctaccgatttccctggcgtttctcgtccgcaggatatgggcggtctgggcttctggtacaagtggaacctcggctggatgcatgacaccctggactacatgaagctcgacccggtttatcgtcagtatcatcacgataaactgaccttcgggattctctacaactacactgaaaacttcgtcctgccgttgtcgcatgatgaagtggtccacggtaaaaaatcgattctcgaccgcatgccgggcgacgcatggcagaaattcgcgaacctgcgcgcctactatggctggatgtgggcattcccgggcaagaaactactgttcatgggtaacgaatttgcccagggccgcgagtggaaccatgacgccagcctcgactggcatctgttggaaggcggcgataactggcaccacggtgtccagcgtctggtgcgcgatctgaacctcacctaccgccaccataaagcaatgcatgaactggattttgacccgtacggctttgaatggctggtggtggatgacaaagaacgctcggtgctgatctttgtgcgtcgcgataaagagggtaacgaaatcatcgttgccagtaactttacgccggtaccgcgtcatgattatcgcttcggcataaaccagccgggcaaatggcgtgaaatcctcaataccgattccatgcactatcacggcagtaatgcaggcaatggcggcacggtacacagcgatgagattgccagccacggtcgtcagcattcactaagcctgacgctaccaccgctggccactatctggctggttcgggaggcagaatgataa
BBa_J04500_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa
BBa_K1618003_sequence
1
atgaatgtgttgagtttaggcagctctagtggtgttgtctggggccgtgtaccgattaccgccccggctggagctgcgaccggcgttacctcacgcgcggacgctcactcccagatgcgccgctatgcacagaccgggcctactgcgaaactgagttcagctccgatgaccacaatgtggggcgcgccgctccaccggcgttggcgcggaagtcgcttgcgggatccgcgtcaggcgaaatttttaacgctggcatcccttaagtgggtacttgcgaaccgtgcgtacactccttggtacttggtccgttactggcgtctgctgcgtttcaaactggcaaatcctcatattattactcgcggcatggtctttctcggtaaaggtgttgagatccatgcaacaccggaattagcgcagttagaaattggccgttgggtgcatattggcgataagaataccattcgcgctcatgagggttcgctccgcttcggtgacaaggtggtgttgggtcgcgacaacgttatcaatacataccttgacattgagatcggcgatagtgtactgatggcggactggtgctatatttgcgatttcgaccatcgcatggatgacatcacactgccaattaaagatcaggggatcattaaaagtccagttcgcattgggccggatacctggatcggggtcaaggtatctgttctgcgtgggacaacgattggccgtggatgtgtcttgggatcgcacgccgtggtgcgtggtgctatcccggactactcgattgctgtgggcgcaccggcaaaagtggttaagaatcgtcagctgtcgtgggaagcgtctgccgcacagcgggccgaactggcggcggcattagctgatatcgaacgcaaaaaagccgcccggtaataa
BBa_K1618022_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgtccgatcgtatcgatagagatgtgattaacgcgctaattgcaggccattttgcggatcctttttccgtactgggaatgcataaaaccaccgcgggactggaagtccgtgcccttttacccgacgctaccgatgtgtgggtgattgaaccgaaaaccgggcgcaaactcgcaaaactggagtgtctcgactcacggggattctttagcggcgtcattccgcgacgtaagaattttttccgctatcagttggctgttgtctggcatggtcagcaaaacctgattgatgatccttaccgttttggtccgctaatccaggaaatggatgcctggctattatctgaaggtactcacctgcgcccgtatgaaaccttaggcgcgcatgcagatactatggatggcgtcacaggtacgcgtttctctgtctgggctccaaacgcccgtcgggtgtcggtggttgggcaattcaactactgggacggtcgccgtcacccgatgcgcctgcgtaaagagagcggcatctgggaactgtttatccctggggcgcataacggtcagctctataaatacgagatgattgatgccaatggcaacttgcgtctgaagtccgacccttatgcctttgaagcgcaaatgcgcccggaaaccgcgtcacttatttgcgggctgccggaaaaggttgtacagactgaagagcgcaaaaaagcgaatcagtttgatgcgccaatctctatttatgaagttcacctgggttcctggcgtcgccacaccgacaacaatttctggttgagctaccgcgagctggccgatcaactggtgccttatgctaaatggatgggctttacccacctcgaactactgcccattaacgagcatcccttcgatggcagttggggttatcagccaaccggcctgtatgcgccaacccgccgttttggtactcgcgacgacttccgttatttcattgatgccgcacacgcagctggtctgaacgtgattctcgactgggtgccaggccacttcccgactgatgactttgcgcttgccgaatttgatggcacgaacttgtatgaacacagcgatccgcgtgaaggctatcatcaggactggaacacgctgatctacaactatggtcgccgtgaagtcagtaacttcctcgtcggtaacgcgctttactggattgaacgttttggtattgatgcgctgcgcgtcgatgcggtggcgtcaatgatttatcgcgactacagccgtaaagagggggagtggatcccgaacgaatttggcgggcgcgagaatcttgaagcgattgagttcttgcgtaataccaaccgtattcttggtgagcaggtttccggtgcggtgacaatggctgaggagtctaccgatttccctggcgtttctcgtccgcaggatatgggcggtctgggcttctggtacaagtggaacctcggctggatgcatgacaccctggactacatgaagctcgacccggtttatcgtcagtatcatcacgataaactgaccttcgggattctctacaactacactgaaaacttcgtcctgccgttgtcgcatgatgaagtggtccacggtaaaaaatcgattctcgaccgcatgccgggcgacgcatggcagaaattcgcgaacctgcgcgcctactatggctggatgtgggcattcccgggcaagaaactactgttcatgggtaacgaatttgcccagggccgcgagtggaaccatgacgccagcctcgactggcatctgttggaaggcggcgataactggcaccacggtgtccagcgtctggtgcgcgatctgaacctcacctaccgccaccataaagcaatgcatgaactggattttgacccgtacggctttgaatggctggtggtggatgacaaagaacgctcggtgctgatctttgtgcgtcgcgataaagagggtaacgaaatcatcgttgccagtaactttacgccggtaccgcgtcatgattatcgcttcggcataaaccagccgggcaaatggcgtgaaatcctcaataccgattccatgcactatcacggcagtaatgcaggcaatggcggcacggtacacagcgatgagattgccagccacggtcgtcagcattcactaagcctgacgctaccaccgctggccactatctggctggttcgggaggcagaatgataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z