LOCUS       BBa_K1618026            2203 bp    DNA     linear       26-Dec-2024
DEFINITION  GlgX with IPTG-inducible promoter
ACCESSION   BBa_K1618026
VERSION     BBa_K1618026.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..2203
                     /label=BBa_K1618025
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga cacaactcgc
      241 cattggcaaa cccactcccc tcggcgcgca ttacgacggt cagggcgtca acttcacact
      301 tttctccgct catgccgagc gggtagagct gtgtgtcttt gatgccaatg gccaggaaca
      361 tcgctatgac ttgccagggc acagtggcga catttggcac ggttatctgc cggatgcgcg
      421 cccgggtttg cgttatggtt atcgcgttca tggcccctgg caacctgccg aggggcatcg
      481 ctttaacccg gcgaagttgt tgattgatcc ttgcgcgcgg caaattgacg gggagtttaa
      541 agataacccg ctgctgcacg ccggtcataa tgaacctgac tatcgcgaca acgccgccat
      601 tgcgccgaaa tgcgtagtgg tggttgatca ctatgactgg gaagatgatg ccccgccgcg
      661 cacgccgtgg ggcagcacca tcatttatga agcccatgtc aaaggattaa cgtatctgca
      721 cccggagatc ccggtcgaga tccgcggcac ttataaagcc ctagggcatc cggtgatgat
      781 caactatttg aaacagttgg gcattactgc gctggagttg ctgccggtgg cacaatttgc
      841 cagcgaacca cgtctgcaac gcatggggct tagtaactac tgggggtaca acccggtggc
      901 gatgtttgcg ctgcatccgg cgtatgcctg ctcgccagaa acggcgctgg atgagtttcg
      961 cgatgcaatc aaagcactgc ataaagcggg tatcgaagtc attcttgata tcgtgctcaa
     1021 ccatagtgcg gaactggacc tcgacggccc gttattctcg ctgcgtggga tcgacaaccg
     1081 tagctattat tggataagag aggacggcga ttatcacaac tggaccggtt gtggtaacac
     1141 gctcaatttg agtcatccgg cggtggtaga ttatgccagc gcctgcctgc gttattgggt
     1201 agaaacctgc cacgtcgatg gtttccgctt tgatctggcg gcagtcatgg gccgtacgcc
     1261 agagtttcgt caggatgcgc cgttgtttac cgctatccag aactgcccgg tgctctcgca
     1321 ggtgaagtta attgctgaac cgtgggatat cgctcctggt ggttatcagg tgggaaattt
     1381 cccgccgctg tttgccgagt ggaacgatca tttccgcgat gcagcccgtc gtttctggct
     1441 gcattatgat ttacctctgg gagcgtttgc cgggcgtttt gctgcctcca gcgatgtttt
     1501 taaacgtaat gatcgtctgc cgagtgccgc gattaatctc gtcaccgcgc atgacggttt
     1561 tacgcttcgc gactgcgttt gcttcaacca taaacacaat gaagcaaacg gagaagaaaa
     1621 tcgcgacggg accaacaaca attacagtaa caatcatggt aaagaagggt taggcggtac
     1681 tcttgatctg gttgaacggc ggcgcgacag cattcacgcc ctgttaacaa cgttgttgct
     1741 ctcccagggt acgccgatgt tactggccgg tgacgaacat ggtcacagcc agcatggcaa
     1801 taacaatgcc tactgtcagg ataaccaatt aacctggttg gactggtcgc aggcaagcag
     1861 tggtttaacc gcatttaccg ccgcgttaat ccatctgcgc aagcgcattc ccgctttgat
     1921 ggagaatcgc tggtgggaag aaggcgacgg caatgtccgt tggctaaatc gatatgctca
     1981 acctttaagc acggatgagt ggcaaaacgg gccgaaacag ctgcaaattc tgctctcgga
     2041 tcgctttttg atcgcaatta atgccacgct tgaggtaaca gagattgttt tacctgctgg
     2101 ggagtggcac gccattcccc cattcgctgg agaggataac ccagtgatta cggctgtctg
     2161 gcagggacct gcacacggat tgtgtgtgtt ccagagatga taa
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