LOCUS BBa_K1618027 3502 bp DNA linear 22-Nov-2024 DEFINITION GlgB-Rv3037c with IPTG-inducible promoter ACCESSION BBa_K1618027 VERSION BBa_K1618027.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 misc_feature 1..220 /label=BBa_J04500 CDS 227..2416 /label=BBa_K1618000 CDS 1..2416 /label=BBa_K1618022 CDS 2423..3502 /label=BBa_K1618004 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt ccgatcgtat 241 cgatagagat gtgattaacg cgctaattgc aggccatttt gcggatcctt tttccgtact 301 gggaatgcat aaaaccaccg cgggactgga agtccgtgcc cttttacccg acgctaccga 361 tgtgtgggtg attgaaccga aaaccgggcg caaactcgca aaactggagt gtctcgactc 421 acggggattc tttagcggcg tcattccgcg acgtaagaat tttttccgct atcagttggc 481 tgttgtctgg catggtcagc aaaacctgat tgatgatcct taccgttttg gtccgctaat 541 ccaggaaatg gatgcctggc tattatctga aggtactcac ctgcgcccgt atgaaacctt 601 aggcgcgcat gcagatacta tggatggcgt cacaggtacg cgtttctctg tctgggctcc 661 aaacgcccgt cgggtgtcgg tggttgggca attcaactac tgggacggtc gccgtcaccc 721 gatgcgcctg cgtaaagaga gcggcatctg ggaactgttt atccctgggg cgcataacgg 781 tcagctctat aaatacgaga tgattgatgc caatggcaac ttgcgtctga agtccgaccc 841 ttatgccttt gaagcgcaaa tgcgcccgga aaccgcgtca cttatttgcg ggctgccgga 901 aaaggttgta cagactgaag agcgcaaaaa agcgaatcag tttgatgcgc caatctctat 961 ttatgaagtt cacctgggtt cctggcgtcg ccacaccgac aacaatttct ggttgagcta 1021 ccgcgagctg gccgatcaac tggtgcctta tgctaaatgg atgggcttta cccacctcga 1081 actactgccc attaacgagc atcccttcga tggcagttgg ggttatcagc caaccggcct 1141 gtatgcgcca acccgccgtt ttggtactcg cgacgacttc cgttatttca ttgatgccgc 1201 acacgcagct ggtctgaacg tgattctcga ctgggtgcca ggccacttcc cgactgatga 1261 ctttgcgctt gccgaatttg atggcacgaa cttgtatgaa cacagcgatc cgcgtgaagg 1321 ctatcatcag gactggaaca cgctgatcta caactatggt cgccgtgaag tcagtaactt 1381 cctcgtcggt aacgcgcttt actggattga acgttttggt attgatgcgc tgcgcgtcga 1441 tgcggtggcg tcaatgattt atcgcgacta cagccgtaaa gagggggagt ggatcccgaa 1501 cgaatttggc gggcgcgaga atcttgaagc gattgagttc ttgcgtaata ccaaccgtat 1561 tcttggtgag caggtttccg gtgcggtgac aatggctgag gagtctaccg atttccctgg 1621 cgtttctcgt ccgcaggata tgggcggtct gggcttctgg tacaagtgga acctcggctg 1681 gatgcatgac accctggact acatgaagct cgacccggtt tatcgtcagt atcatcacga 1741 taaactgacc ttcgggattc tctacaacta cactgaaaac ttcgtcctgc cgttgtcgca 1801 tgatgaagtg gtccacggta aaaaatcgat tctcgaccgc atgccgggcg acgcatggca 1861 gaaattcgcg aacctgcgcg cctactatgg ctggatgtgg gcattcccgg gcaagaaact 1921 actgttcatg ggtaacgaat ttgcccaggg ccgcgagtgg aaccatgacg ccagcctcga 1981 ctggcatctg ttggaaggcg gcgataactg gcaccacggt gtccagcgtc tggtgcgcga 2041 tctgaacctc acctaccgcc accataaagc aatgcatgaa ctggattttg acccgtacgg 2101 ctttgaatgg ctggtggtgg atgacaaaga acgctcggtg ctgatctttg tgcgtcgcga 2161 taaagagggt aacgaaatca tcgttgccag taactttacg ccggtaccgc gtcatgatta 2221 tcgcttcggc ataaaccagc cgggcaaatg gcgtgaaatc ctcaataccg attccatgca 2281 ctatcacggc agtaatgcag gcaatggcgg cacggtacac agcgatgaga ttgccagcca 2341 cggtcgtcag cattcactaa gcctgacgct accaccgctg gccactatct ggctggttcg 2401 ggaggcagaa tgataatact agatgcgcgc acgttttggg gaccgggcac cgtggctggt 2461 ggagacgacg ctgcttcgtc gtcgcgccgc cggtaaactt ggcgagctgt gtccgaacgt 2521 aggcgtgagc cagtggttgt ttacggatga ggcgctccag caggcaaccg cggctcccgt 2581 cgcgcgtcac cgtgcgcgtc ggctggccgg gcgcgttgtt cacgacgcga cctgctccat 2641 cggcaccgaa ctggccgcgc tgcgcgagtt ggccgtgcgg gcagttggta gcgatattga 2701 tccagttcgc ctcgcgatgg cgcgccataa tttagccgcc ctcggtatgg aagcggatct 2761 ctgccgcgcg gatgtgttgc acccagtgac ccgcgacgcg gtcgtcgtga ttgatccggc 2821 gcgtcgctcc aacggtcggc gccggtttca tctggcagat taccagccag ggctgggtcc 2881 gctgctggat cgttaccgtg gccgtgatgt ggttgttaaa tgtgctcctg gtatcgactt 2941 cgaagaggtt ggccggctcg gttttgaagg cgagattgag gtgatcagct accgcggtgg 3001 tgttcgcgaa gcctgtctgt ggagcgcggg tcttgcgggc tcaggaatcc gccgccgtgc 3061 gagcattctg gattctggtg aacagatcgg cgatgatgaa ccggatgact gtggagttcg 3121 cccggccggc aaatggattg tggatcccga cggtgcggtg gtgcgtgctg gcttagtgcg 3181 caattatggt gcacgccatg gcctgtggca gcttgaccct caaattgcct atctgagtgg 3241 tgaccgtctg cctccagcgc tgcgcgggtt tgaggtactt gagcagctgg cgtttgatga 3301 acgtcgcctg cgccaggtgc tctctgcact tgattgcggt gctgcggaaa ttctggtgcg 3361 tggggtcgca atcgaccctg atgcgctgcg ccgtcgtctg cgcctccgcg gctcacgtcc 3421 gcttgcagtt gtaatcaccc gcattggtgc cggctccctg tcacatgtga ccgcatatgt 3481 ttgccgccca tcgcggtaat aa //