BBa_K1618046
type: ComponentDefinition
persistent identity: BBa K1618046
display id: BBa_K1618046
version: 1
was generated by: iGEM to SBOL conversion
description: THL.BHBD.CRO.BCD.EFb.EFa.BUK.PTB
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T07:09:02Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: .
discontinued: false
dominant: false
group u list: _2035_
m user id: 24691
owner id: 24691
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: .
star: false
creator: Kieran Rustage
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1618021
component: BBa_K1618039
component: BBa_K1618038
sequence annotation: BBa_K1618021
sequence annotation: BBa_K1618038
sequence annotation: BBa_K1618039
sequence: https://synbiohub.org/public/igem/BBa_K1618046_sequence/1

BBa_K1618021
type: Component
persistent identity: component2454110
display id: component2454110
version: 1
top level: BBa_K1618046
definition: BBa_K1618021
access: public

BBa_K1618039
type: Component
persistent identity: component2454112
display id: component2454112
version: 1
top level: BBa_K1618046
definition: BBa_K1618039
access: public

BBa_K1618038
type: Component
persistent identity: component2454111
display id: component2454111
version: 1
top level: BBa_K1618046
definition: BBa_K1618038
access: public

BBa_K1618021
type: SequenceAnnotation
persistent identity: annotation2454110
display id: annotation2454110
version: 1
top level: BBa_K1618046
location: https://synbiohub.org/public/igem/BBa_K1618046/annotation2454110/range2454110/1
component: BBa_K1618021

https://synbiohub.org/public/igem/BBa_K1618046/annotation2454110/range2454110/1
type: Range
persistent identity: range2454110
display id: range2454110
version: 1
top level: BBa_K1618046
direction: direction/reverse
start: 1
end: 6101
orientation: inline

BBa_K1618038
type: SequenceAnnotation
persistent identity: annotation2454111
display id: annotation2454111
version: 1
top level: BBa_K1618046
location: https://synbiohub.org/public/igem/BBa_K1618046/annotation2454111/range2454111/1
component: BBa_K1618038

https://synbiohub.org/public/igem/BBa_K1618046/annotation2454111/range2454111/1
type: Range
persistent identity: range2454111
display id: range2454111
version: 1
top level: BBa_K1618046
direction: direction/reverse
start: 6110
end: 7198
orientation: inline

BBa_K1618039
type: SequenceAnnotation
persistent identity: annotation2454112
display id: annotation2454112
version: 1
top level: BBa_K1618046
location: https://synbiohub.org/public/igem/BBa_K1618046/annotation2454112/range2454112/1
component: BBa_K1618039

https://synbiohub.org/public/igem/BBa_K1618046/annotation2454112/range2454112/1
type: Range
persistent identity: range2454112
display id: range2454112
version: 1
top level: BBa_K1618046
direction: direction/reverse
start: 7207
end: 8141
orientation: inline

BBa_K1618038
type: ComponentDefinition
persistent identity: BBa K1618038
display id: BBa_K1618038
version: 1
was generated by: iGEM to SBOL conversion
description: Butyrate Kinase
created: 2015-09-13T11:00:00Z
modified: 2015-09-16T01:35:45Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: .
discontinued: false
dominant: false
group u list: _2035_
m user id: 24779
owner id: 24691
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: .
star: false
creator: Kieran Rustage
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1618038_sequence/1

BBa_K1618039
type: ComponentDefinition
persistent identity: BBa K1618039
display id: BBa_K1618039
version: 1
was generated by: iGEM to SBOL conversion
description: ptB
created: 2015-09-13T11:00:00Z
modified: 2015-09-16T03:05:28Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: .
discontinued: false
dominant: false
group u list: _2035_
m user id: 24779
owner id: 24691
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: .
star: false
creator: Kieran Rustage
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1618039_sequence/1

BBa_K1618021
type: ComponentDefinition
persistent identity: BBa K1618021
display id: BBa_K1618021
version: 1
was generated by: iGEM to SBOL conversion
description: Plac-THL-CRO-BHBD-BCD-EFTb-EFTa
created: 2015-08-27T11:00:00Z
modified: 2016-01-25T11:20:44Z
mutable provenance: Coprococcus sp. L2-50
owned by: james
owned by: myers
mutable description: Acetate to Butyrate pathway with IPTG inducible Promoter
discontinued: false
dominant: false
experience: experience/None
group u list: _2035_
m user id: 4206
owner id: 24691
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: .
star: false
creator: Kieran Rustage
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1618042
component: BBa_K1618041
component: BBa_K1618044
component: BBa_K1618043
component: BBa_K1618040
component: BBa_J04500
component: BBa_K1618045
sequence annotation: BBa_J04500
sequence annotation: BBa_K1618040
sequence annotation: BBa_K1618041
sequence annotation: BBa_K1618042
sequence annotation: BBa_K1618043
sequence annotation: BBa_K1618044
sequence annotation: BBa_K1618045
sequence: https://synbiohub.org/public/igem/BBa_K1618021_sequence/1

BBa_K1618042
type: Component
persistent identity: component2454090
display id: component2454090
version: 1
top level: BBa_K1618021
definition: BBa_K1618042
access: public

BBa_K1618041
type: Component
persistent identity: component2454089
display id: component2454089
version: 1
top level: BBa_K1618021
definition: BBa_K1618041
access: public

BBa_K1618044
type: Component
persistent identity: component2454092
display id: component2454092
version: 1
top level: BBa_K1618021
definition: BBa_K1618044
access: public

BBa_K1618043
type: Component
persistent identity: component2454091
display id: component2454091
version: 1
top level: BBa_K1618021
definition: BBa_K1618043
access: public

BBa_K1618040
type: Component
persistent identity: component2454088
display id: component2454088
version: 1
top level: BBa_K1618021
definition: BBa_K1618040
access: public

BBa_J04500
type: Component
persistent identity: component2454087
display id: component2454087
version: 1
top level: BBa_K1618021
definition: BBa_J04500
access: public

BBa_K1618045
type: Component
persistent identity: component2454093
display id: component2454093
version: 1
top level: BBa_K1618021
definition: BBa_K1618045
access: public

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2454087
display id: annotation2454087
version: 1
top level: BBa_K1618021
location: https://synbiohub.org/public/igem/BBa_K1618021/annotation2454087/range2454087/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1618021/annotation2454087/range2454087/1
type: Range
persistent identity: range2454087
display id: range2454087
version: 1
top level: BBa_K1618021
direction: direction/reverse
start: 1
end: 220
orientation: inline

BBa_K1618040
type: SequenceAnnotation
persistent identity: annotation2454088
display id: annotation2454088
version: 1
top level: BBa_K1618021
location: https://synbiohub.org/public/igem/BBa_K1618021/annotation2454088/range2454088/1
component: BBa_K1618040

https://synbiohub.org/public/igem/BBa_K1618021/annotation2454088/range2454088/1
type: Range
persistent identity: range2454088
display id: range2454088
version: 1
top level: BBa_K1618021
direction: direction/reverse
start: 229
end: 1430
orientation: inline

BBa_K1618041
type: SequenceAnnotation
persistent identity: annotation2454089
display id: annotation2454089
version: 1
top level: BBa_K1618021
location: https://synbiohub.org/public/igem/BBa_K1618021/annotation2454089/range2454089/1
component: BBa_K1618041

https://synbiohub.org/public/igem/BBa_K1618021/annotation2454089/range2454089/1
type: Range
persistent identity: range2454089
display id: range2454089
version: 1
top level: BBa_K1618021
direction: direction/reverse
start: 1437
end: 2306
orientation: inline

BBa_K1618042
type: SequenceAnnotation
persistent identity: annotation2454090
display id: annotation2454090
version: 1
top level: BBa_K1618021
location: https://synbiohub.org/public/igem/BBa_K1618021/annotation2454090/range2454090/1
component: BBa_K1618042

https://synbiohub.org/public/igem/BBa_K1618021/annotation2454090/range2454090/1
type: Range
persistent identity: range2454090
display id: range2454090
version: 1
top level: BBa_K1618021
direction: direction/reverse
start: 2313
end: 3101
orientation: inline

BBa_K1618043
type: SequenceAnnotation
persistent identity: annotation2454091
display id: annotation2454091
version: 1
top level: BBa_K1618021
location: https://synbiohub.org/public/igem/BBa_K1618021/annotation2454091/range2454091/1
component: BBa_K1618043

https://synbiohub.org/public/igem/BBa_K1618021/annotation2454091/range2454091/1
type: Range
persistent identity: range2454091
display id: range2454091
version: 1
top level: BBa_K1618021
direction: direction/reverse
start: 3108
end: 4271
orientation: inline

BBa_K1618044
type: SequenceAnnotation
persistent identity: annotation2454092
display id: annotation2454092
version: 1
top level: BBa_K1618021
location: https://synbiohub.org/public/igem/BBa_K1618021/annotation2454092/range2454092/1
component: BBa_K1618044

https://synbiohub.org/public/igem/BBa_K1618021/annotation2454092/range2454092/1
type: Range
persistent identity: range2454092
display id: range2454092
version: 1
top level: BBa_K1618021
direction: direction/reverse
start: 4278
end: 5060
orientation: inline

BBa_K1618045
type: SequenceAnnotation
persistent identity: annotation2454093
display id: annotation2454093
version: 1
top level: BBa_K1618021
location: https://synbiohub.org/public/igem/BBa_K1618021/annotation2454093/range2454093/1
component: BBa_K1618045

https://synbiohub.org/public/igem/BBa_K1618021/annotation2454093/range2454093/1
type: Range
persistent identity: range2454093
display id: range2454093
version: 1
top level: BBa_K1618021
direction: direction/reverse
start: 5067
end: 6101
orientation: inline

BBa_K1618040
type: ComponentDefinition
persistent identity: BBa K1618040
display id: BBa_K1618040
version: 1
was generated by: iGEM to SBOL conversion
description: THL
created: 2015-09-13T11:00:00Z
modified: 2015-09-16T01:53:15Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: .
discontinued: false
dominant: false
group u list: _2035_
m user id: 24779
owner id: 24691
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: .
star: false
creator: Kieran Rustage
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1618040_sequence/1

BBa_K1618041
type: ComponentDefinition
persistent identity: BBa K1618041
display id: BBa_K1618041
version: 1
was generated by: iGEM to SBOL conversion
description: BHBD
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T06:55:42Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: .
discontinued: false
dominant: false
group u list: _2035_
m user id: 24691
owner id: 24691
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: .
star: false
creator: Kieran Rustage
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1618041_sequence/1

BBa_K1618042
type: ComponentDefinition
persistent identity: BBa K1618042
display id: BBa_K1618042
version: 1
was generated by: iGEM to SBOL conversion
description: Cro
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T06:57:14Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: .
discontinued: false
dominant: false
group u list: _2035_
m user id: 24691
owner id: 24691
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: .
star: false
creator: Kieran Rustage
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1618042_sequence/1

BBa_K1618043
type: ComponentDefinition
persistent identity: BBa K1618043
display id: BBa_K1618043
version: 1
was generated by: iGEM to SBOL conversion
description: BCD
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T05:21:50Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: .
discontinued: false
dominant: false
group u list: _2035_
m user id: 24691
owner id: 24691
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: .
star: false
creator: Kieran Rustage
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1618043_sequence/1

BBa_K1618044
type: ComponentDefinition
persistent identity: BBa K1618044
display id: BBa_K1618044
version: 1
was generated by: iGEM to SBOL conversion
description: EFb
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T07:00:14Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: .
discontinued: false
dominant: false
group u list: _2035_
m user id: 24691
owner id: 24691
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: .
star: false
creator: Kieran Rustage
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1618044_sequence/1

BBa_K1618045
type: ComponentDefinition
persistent identity: BBa K1618045
display id: BBa_K1618045
version: 1
was generated by: iGEM to SBOL conversion
description: EFa
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T07:01:15Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: .
discontinued: false
dominant: false
group u list: _2035_
m user id: 24691
owner id: 24691
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: .
star: false
creator: Kieran Rustage
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1618045_sequence/1

BBa_J04500
type: ComponentDefinition
persistent identity: BBa J04500
display id: BBa_J04500
version: 1
was generated by: iGEM to SBOL conversion
description: IPTG inducible promoter with RBS
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: R0010.B0034
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 326
owning group id: 16
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Kristen DeCelle
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J04500_sequence/1

BBa_B0034
type: Component
persistent identity: component1508159
display id: component1508159
version: 1
top level: BBa_J04500
definition: BBa_B0034
access: public

BBa_R0010
type: Component
persistent identity: component1508149
display id: component1508149
version: 1
top level: BBa_J04500
definition: LacI
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1508149
display id: annotation1508149
version: 1
top level: BBa_J04500
location: https://synbiohub.org/public/igem/BBa_J04500/annotation1508149/range1508149/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04500/annotation1508149/range1508149/1
type: Range
persistent identity: range1508149
display id: range1508149
version: 1
top level: BBa_J04500
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1508159
display id: annotation1508159
version: 1
top level: BBa_J04500
location: https://synbiohub.org/public/igem/BBa_J04500/annotation1508159/range1508159/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04500/annotation1508159/range1508159/1
type: Range
persistent identity: range1508159
display id: range1508159
version: 1
top level: BBa_J04500
direction: direction/forward
start: 209
end: 220
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

https://synbiohub.org/public/igem/BBa_K1618046_sequence/1
type: Sequence
persistent identity: BBa K1618046 sequence
display id: BBa_K1618046_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagaggaattcgcggccgcttctagatggcgaaaaaaatcgtactggcaggtgcgtgccgtacggcaatcggcaccatgggcggcagcctgtcaaccacgccagcgccggtattaggttccatcgtaattaaggaggcgctgaaccgtgccggagtgccagctgatcaggtcgatcacgtgtatatgggctgcgtgatccaagctggattagggcagaatgttgcccgccaggcttcgatcaaagccggtttgcccatcgagacgacggcggtaacggtgaacgttgtctgcggttctggcctgaattgcgtaaacatggcggcccagatgattgaagcgggcgatgcggacattgttgttgcaggtggaatggaaaatatgtccatggcgccgtacgccgtcatgcagggccgtttcggatatcgcatgaatgatggcaaactggtggacaccatggtgcatgatgccttgtgggacgcatttaatgattatcacatgatcaaaaccgcagataacatttgtgaacagtgggggttaacccgcgaagaactggatgagttcgccgctaaatcacagcagaaagcagtcgcggcacaggaaagcggggccttcgacgctgaaatcgtcccggttatggttaaaaaaaaaaaagaaatggtagaatttaaaaaagatgaaggcccgcgtcccggtacagtggataagcaggtcaacccaaatggcggggcgattgccctgggtcatcccgttggcgcatccggctgtcgcatcttagtgacgctgctgcacgaaatgcaggccaaaggtgccaaaacaggactggcaaccctgtgtatcggcggcggtatgggctgtagcacggtcgttaagattgaggataccgtagaaacgttatcccgtctgcgcactattaacccgaatggctttgtgaccgcgggcaatgctagcggaattaatgatggtgcagccgcgatcgtcgttatgagcgaagagaaagctaaagaactcggcgtgaaaccaatggcgacgtgggtggcaggtgcactgggcggtgttgacccctcgattatgggtattggcccggtggcatcgactaagaaagttatggccaaaaccggtctgaaaatcgaggacttcgacattattgaagcgaacgaagcatttgcggcgcagtctgttgcggtggccaaagatctgggctttgattactagatgaaagtgggtgttatcggtgctggcaccatgggccagggcattgcaaaagctttcgcccaagttgacggttatgaagtagcgctgtgcgatattaaacaagaatgggcagaaggtggaaaagataaaatcgcgaaaggatatgcacggttggtcgagaaaggcaaaatgacgcaagagaaagtggatggcattttggcctcgattacaccgggcatcaaagaggatctctgtgccgattgcgatctgattgtcgaagccgcgtttgaaaatatggaagtcaaaaagaccacattttcggaactggacaagatttgtaaaccggaatgcgtgttcgcgtccaataccagttctttgtcaatcaccgaaattggcaatggcttaactcgcccaatgatcggtatgcacttcttcaatccggccgatcgtatgaaattgatcgaagttatcgcgggtgttaacaccccagcagaaaccgtcgataaaattgtgaaaatttctgaagaaattggtaaaactcctgtgcaagtaaatgaagccgcaggattcgtggtgaaccgcatt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1618038_sequence/1
type: Sequence
persistent identity: BBa K1618038 sequence
display id: BBa_K1618038_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtatagattactaataatcaatcctggctcgacctcaactaaaattggtatttatgacgatgaaaaagagatatttgagaaaactttaagacattcagctgaagagatagaaaaatataacactatatttgatcaatttcaattcagaaagaatgtaattttagatgcgttaaaagaagcaaacatagaagtaagttctttaaatgctgtagttggaagaggcggactcttaaagccaatagtaagtggaacttatgcagtaaatcaaaaaatgcttgaagatcttaaagtaggagttcaaggtcagcatgcgtcaaatcttggtggaattattgcaaatgaaatagcaaaagaaataaatgttccagcatacatagttgatccagttgttgtggatgagcttgatgaagtttcaagaatatcaggaatggctgacattccaagaaaaagtatattccatgcattaaatcaaaaagcagttgctagaagatatgcaaaagaagttggaaaaaaatacgaagatcttaatttaatcgtagtccacatgggtggaggtacttcagtaggtactcataaagatggtagagtaatagaagttaataatacacttgatggagaaggtccattctcaccagaaagaagtggtggagttccaataggagatcttgtaagattgtgcttcagcaacaaatatacttatgaagaagtaatgaaaaagataaacggcaaaggcggagttgttagttacttaaatactatcgattttaaggctgtagttgataaagctcttgaaggagataagaaatgtgcacttatatatgaagctttcacattccaggtagcaaaagagataggaaaatgttcaaccgttttaaaaggaaatgtagatgcaataatcttaacaggcggaattgcgtacaacgagcatgtatgtaatgccatagaggatagagtaaaattcatagcacctgtagttagatatggtggagaagatgaacttcttgcacttcgagaaggtggacttagagttttaagaggagaagaaaaagctaaggaatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1618039_sequence/1
type: Sequence
persistent identity: BBa K1618039 sequence
display id: BBa_K1618039_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgattaagagttttaatgaaattatcatgaaggtaaagagcaaagaaatgaaaaaagttgctgttgctgtagcacaagacgagccagtacttgaagcagtaagagatgctaagaaaaatggtattgcagatgctattcttgttggagaccatgacgaaatcgtgtcaatcgcgcttaaaataggaatggatgtaaatgattttgaaatagtaaacgagcctaacgttaagaaagctgctttaaaggcagtagagcttgtatcaactggaaaagctgatatattaatgaatggacttgtaaatacagcaactttcttaaagatctgcatattaaacaaagaagttggacttagaacaggaaaaactatgtctcacgttgcagtatttgaaactgagacatctgatagactatcatttttaacagatgttgctttcaatacttatcctgaattaaaggaaaaaattgatatagtaaacaattcagttaaggttgcacatgcaataggaattgtaaatccaaaggttgctccaatttgtgcagttgaggttataaaccctaaaatgccatcaacacttgatgcagcaatgctttcaaaaatgagtgacagaggacaaattaaaggttgtgtagttgacggacctttagcacttgatatagctttatcagaagaagcagcacatcataagggagtaacaggagaagttgctggaaaagctgatatcttcttaatgccaaacatagaaacaggaaatgtaatgtataagactttaacatatacaactgattcaaaaaatggaggaatcttagttggaacttctgcaccagttgttttaacttcaagagctgacagccatgaaacaaaaatgaactctatagcacttgcagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1618021_sequence/1
type: Sequence
persistent identity: BBa K1618021 sequence
display id: BBa_K1618021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagaggaattcgcggccgcttctagatggcgaaaaaaatcgtactggcaggtgcgtgccgtacggcaatcggcaccatgggcggcagcctgtcaaccacgccagcgccggtattaggttccatcgtaattaaggaggcgctgaaccgtgccggagtgccagctgatcaggtcgatcacgtgtatatgggctgcgtgatccaagctggattagggcagaatgttgcccgccaggcttcgatcaaagccggtttgcccatcgagacgacggcggtaacggtgaacgttgtctgcggttctggcctgaattgcgtaaacatggcggcccagatgattgaagcgggcgatgcggacattgttgttgcaggtggaatggaaaatatgtccatggcgccgtacgccgtcatgcagggccgtttcggatatcgcatgaatgatggcaaactggtggacaccatggtgcatgatgccttgtgggacgcatttaatgattatcacatgatcaaaaccgcagataacatttgtgaacagtgggggttaacccgcgaagaactggatgagttcgccgctaaatcacagcagaaagcagtcgcggcacaggaaagcggggccttcgacgctgaaatcgtcccggttatggttaaaaaaaaaaaagaaatggtagaatttaaaaaagatgaaggcccgcgtcccggtacagtggataagcaggtcaacccaaatggcggggcgattgccctgggtcatcccgttggcgcatccggctgtcgcatcttagtgacgctgctgcacgaaatgcaggccaaaggtgccaaaacaggactggcaaccctgtgtatcggcggcggtatgggctgtagcacggtcgttaagattgaggataccgtagaaacgttatcccgtctgcgcactattaacccgaatggctttgtgaccgcgggcaatgctagcggaattaatgatggtgcagccgcgatcgtcgttatgagcgaagagaaagctaaagaactcggcgtgaaaccaatggcgacgtgggtggcaggtgcactgggcggtgttgacccctcgattatgggtattggcccggtggcatcgactaagaaagttatggccaaaaccggtctgaaaatcgaggacttcgacattattgaagcgaacgaagcatttgcggcgcagtctgttgcggtggccaaagatctgggctttgattactagatgaaagtgggtgttatcggtgctggcaccatgggccagggcattgcaaaagctttcgcccaagttgacggttatgaagtagcgctgtgcgatattaaacaagaatgggcagaaggtggaaaagataaaatcgcgaaaggatatgcacggttggtcgagaaaggcaaaatgacgcaagagaaagtggatggcattttggcctcgattacaccgggcatcaaagaggatctctgtgccgattgcgatctgattgtcgaagccgcgtttgaaaatatggaagtcaaaaagaccacattttcggaactggacaagatttgtaaaccggaatgcgtgttcgcgtccaataccagttctttgtcaatcaccgaaattggcaatggcttaactcgcccaatgatcggtatgcacttcttcaatccggccgatcgtatgaaattgatcgaagttatcgcgggtgttaacaccccagcagaaaccgtcgataaaattgtgaaaatttctgaagaaattggtaaaactcctgtgcaagtaaatgaagccgcaggattcgtggtgaaccgcatt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1618040_sequence/1
type: Sequence
persistent identity: BBa K1618040 sequence
display id: BBa_K1618040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcgaaaaaaatcgtactggcaggtgcgtgccgtacggcaatcggcaccatgggcggcagcctgtcaaccacgccagcgccggtattaggttccatcgtaattaaggaggcgctgaaccgtgccggagtgccagctgatcaggtcgatcacgtgtatatgggctgcgtgatccaagctggattagggcagaatgttgcccgccaggcttcgatcaaagccggtttgcccatcgagacgacggcggtaacggtgaacgttgtctgcggttctggcctgaattgcgtaaacatggcggcccagatgattgaagcgggcgatgcggacattgttgttgcaggtggaatggaaaatatgtccatggcgccgtacgccgtcatgcagggccgtttcggatatcgcatgaatgatggcaaactggtggacaccatggtgcatgatgccttgtgggacgcatttaatgattatcacatgatcaaaaccgcagataacatttgtgaacagtgggggttaacccgcgaagaactggatgagttcgccgctaaatcacagcagaaagcagtcgcggcacaggaaagcggggccttcgacgctgaaatcgtcccggttatggttaaaaaaaaaaaagaaatggtagaatttaaaaaagatgaaggcccgcgtcccggtacagtggataagcaggtcaacccaaatggcggggcgattgccctgggtcatcccgttggcgcatccggctgtcgcatcttagtgacgctgctgcacgaaatgcaggccaaaggtgccaaaacaggactggcaaccctgtgtatcggcggcggtatgggctgtagcacggtcgttaagattgaggataccgtagaaacgttatcccgtctgcgcactattaacccgaatggctttgtgaccgcgggcaatgctagcggaattaatgatggtgcagccgcgatcgtcgttatgagcgaagagaaagctaaagaactcggcgtgaaaccaatggcgacgtgggtggcaggtgcactgggcggtgttgacccctcgattatgggtattggcccggtggcatcgactaagaaagttatggccaaaaccggtctgaaaatcgaggacttcgacattattgaagcgaacgaagcatttgcggcgcagtctgttgcggtggccaaagatctgggctttgattaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1618041_sequence/1
type: Sequence
persistent identity: BBa K1618041 sequence
display id: BBa_K1618041_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaagtgggtgttatcggtgctggcaccatgggccagggcattgcaaaagctttcgcccaagttgacggttatgaagtagcgctgtgcgatattaaacaagaatgggcagaaggtggaaaagataaaatcgcgaaaggatatgcacggttggtcgagaaaggcaaaatgacgcaagagaaagtggatggcattttggcctcgattacaccgggcatcaaagaggatctctgtgccgattgcgatctgattgtcgaagccgcgtttgaaaatatggaagtcaaaaagaccacattttcggaactggacaagatttgtaaaccggaatgcgtgttcgcgtccaataccagttctttgtcaatcaccgaaattggcaatggcttaactcgcccaatgatcggtatgcacttcttcaatccggccgatcgtatgaaattgatcgaagttatcgcgggtgttaacaccccagcagaaaccgtcgataaaattgtgaaaatttctgaagaaattggtaaaactcctgtgcaagtaaatgaagccgcaggattcgtggtgaaccgcattctgattccgatgatcaatgaggcggcgtttatcaaaatggaaggcgtcagtgacattgcgggtatcgatgcagctatgaaactgggtgccaaccatccgatgggaccgctggaactgggagattttgtggggctggatatctgtctggcaattatggatgtgctttataacgaaacgggtgattcgaaataccgcgcatgcccgcttctccgcaaaatggtacgtggtggtaatctgggcgttaaatctggtaaaggcttttacgtctacaacgccgaccgtactaaaactccagtggacgcgcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1618042_sequence/1
type: Sequence
persistent identity: BBa K1618042 sequence
display id: BBa_K1618042_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggaatttgttacctatgagcaagatggctttgttggagttattacgatcaatcgcccaaaagcgctgaacgcgctgaatagcaccgtgctcgaagaactcgatgcgaccttcaaagctgtggacctgaatacaactcgctgtctcgtcttgactggtgcaggcgaaaaaagcttcgtcgccggtgccgacatcggggaaatgagtacgctgacgaaagcagaaggggaagcatttggtaaaaaaggtaatgatgtgttccgcatgatcgagacttttccgattccagtcatcgccgcggttaacggcttcgctctgggcggcggttgcgagatttcaatgagttgtgatatccgtatttgttccgaaaacgccgtgtttgggcagccagaagtgggtctgggcatcacacctggttttggcgggacgcagcgcctggcgcgcttagtcggggcgggcatggcaaaacaacttatttataccgcccgcaatatcaaggccgatgaggcataccgtattgggcttgtcaacgctgtctatccgctggaagagttgcttcctgccgcgaaaaaaatggccagtattattgctgccaatgcaccgattgctgtgcgcaattgtaaaaaagcgattaacgatggacttcaagtggatatggatcaagcgatcgtggtagaggaaaagttatttggcgattgttttgaaaccgaggaccaaaaagcgggtatgggtaactttctggagaaagacaaggaaaaaaaactgaaagttgtcccctttcagaacaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1618043_sequence/1
type: Sequence
persistent identity: BBa K1618043 sequence
display id: BBa_K1618043_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggacttcgccttagataaaaagtacgaaatggctcagaatttattccgcgaattcgcacagaatgaagtgaaaccgctggctcaggaaatcgatgaaaaccatcgctttccgcgtgaaaccgttgagaaaatggcaaaatacggctttcttgggattcccgtttcaaaagaggacggcggccaaggctgcgacccccttacctatgtcatgtgtgttgaggaacttagcaaggtttgcggtacgaccggtgtaatcgtgtcagcacatactagcctttgtgtagatccgattcaaacctacggcacgccggaccagaaagctaagtacctgccggatctggcttcaggccgtaaattgggtgcctttggtctgactgaaccaggagccgggacggacgcccagggccagcaaaccaaagccgttctggacggggacgagtgggtcctgaatggttctaaatgttttatcacgaacggcaaagaagccgacgtctatattattattgcggttacggggactatcgaaaaacgcggccgtaaacaaaaagaaatctccgctttcatcgttgaaaaggggactcccggttttacctttggtactaaagaaaataagatgggcatttgcggcagcagcacatatgagctgatcttcaccgactgccgtattccgaaggacgcgctgctgggtcaaaaaggcaaaggctttggtattgccatgcatacactggacggcggccgcatcggcattgccgcgcaggcactcggtattgctgaaggtgcactggacatcaccatcgattacgttaaagaacgtaagcaattcggacgctcaatcgctcagttccaaaacacgcagtttgtgctggcagacttggcaacaaaagtagaagcagccaaactgctggtgtataaggccgcaaaaaagaaagaacaatatcaagcggatcataaaacaagctatagcgttgaagccgctatggccaaactgtatgcagcggaagtggcaatggaagttacaacgaaatgcgtgcaactgcacggcggatacggttatatcaaggagtatgatgtagaacgcatgatgcgcgacgctaaaattactgaaatttacgagggcactagcgaagttcaacgtatggttatttccgctaatctgctgaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1618044_sequence/1
type: Sequence
persistent identity: BBa K1618044 sequence
display id: BBa_K1618044_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaagtgattgtttgtattaaacaagtgcctgacaccaagggcggcgttcagtttaatcctgatggtacgttgaatcggggggcgatgctgacaatcatgaacccggacgacaaagcaggcctggaagcggccctgcggctgaaggatcaatatggggcagaagtcacggtgttaacgatgggcctgccgaaagcggcagacgtacttcaggaagcgattgcaatgggtgcagataaaggcgtgttggttaccgaccgggttctcggcggagctgatacctgggccacatcgacaaccatcgccggtgctttacgcaaacttgactacgatctcattatcacgggtcgccaggctattgatggagatactgcacaagtgggtccgcagatcgcggagcatctcggcattccggtgatttcgtacgcacaggaaattaaagtggatggtgactcggtgattgttaaacgccagtatgatgacggctatcatatgctgaaagctaaaatgccctgcctggtgacggcgctgtcagagctgaatgaaccgcgttacatgaccccaggtgggatctttgatgctgtgaatgccgaaattactaccctgggccgcgccgatcttgtcgatgtggatgatagtaatttgggtttgaaaggttccccgaccaaaatcgcgaaagcttcagataaagtgcggaagggcgccggtgaaaaagtcgttccagattctccagacgaagccgttagttacatcgtagggaaactcaaagacaaacatgttatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1618045_sequence/1
type: Sequence
persistent identity: BBa K1618045 sequence
display id: BBa_K1618045_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaactataaaggtgtatttgtgttcgcacaacaggtggataacaagcttagcggtatctcgttcgaactgattggcgaaggtaaacgtttagctgataaattaaacgaaaaggttaccgcggtactgatcggcagcgatgttaaaggccttgtcgatgaactggccgaatatggggcagaccgtgtgatcgtggttgatgatccggaattaaaggaatatcgtaccgaaccatatgctcatgccctggcaagcgttattaatgagtataagcccgaaatcgttctcgtcggcgctacagcgattggccgtgacctggggccgacggtgagtgcgcgcgttgccacgggactgaccgcggactgcacccttctggaaattggtgacttcccattagtcgccttgcccaatcaagaacagaaacacaaccaattacttatgactcggcctgcctttggtggaaacacgattgcgacaattgcttgtcctgacaatcgcccgcaaatggccaccgttcgtccgggcgttatgcaaaaacttgatcgtgtggcaggcaaaaaagcagaggtcattgagtataatccgggttttactcctaacaacaaatacgtcgaaatcttagaaatttcgaaagcagtttctgacattaaagacatcatggacgcgaaaattctggtgtcggggggacggggcgtaggtagcgcagaaaattttaagctgctggatgatctggccgaagttctgggtggccaggtgtcctgctcgcgtgccgtagttgattcgggttggaagccgaaagaattgcaggttgggcagaccgggaaaaccgtgcgccctcaggtctatttcgccatcggcatctcaggtgctatccagcacgtggccggtatggaagaaagtgatattatcatcgccattaataaagatgaagatgcaccgattttcgacgttgctgattacggtgtggtaggcgatttaaataaaatcgtacccgcgttaacagaagccattaaggctgagctggcgaacaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J04500_sequence/1
type: Sequence
persistent identity: BBa J04500 sequence
display id: BBa_J04500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z